DNA, RNA and Protein Synthesis

The syntheses of RNA (transcription), DNA and proteins (translation) are fundamental processes necessary for all life. Transcription begins by uncoiling a section of DNA that will be used as the template and is initiated by RNA polymerase binding to a promoter sequence.

Products
Background
Literature

Inhibitors

Cat No Product Name / Activity
4215 4E1RCat
Protein translation inhibitor; blocks eIF4F subunit interaction
4800 4EGI-1
Inhibitor of eIF4E:eIF4G interaction
1229 Actinomycin D
RNA polymerase inhibitor
2513 Acyclovir
Viral DNA polymerase inhibitor
5211 AM-TS23
DNA polymerase λ and β inhibitor
4025 α-Amanitin
Inhibitor of RNA polymerase II
1290 Anisomycin
Protein synthesis inhibitor
5736 Aphidicolin
DNA polymerase α, δ and ε inhibitor
2981 BIBR 1532
Selective telomerase inhibitor
5417 BMH 21
RNA polymerase 1 inhibitor; also p53 pathway activator
5312 BRACO 19 trihydrochloride
Telomerase inhibitor
4799 Capecitabine
Prodrug of 5-Fluorouracil (Cat. No. 3257). Inhibits DNA synthesis
2626 Carboplatin
Inhibitor of DNA synthesis
4436 8-Chloroadenosine
Cytotoxic nucleoside analog; inhibits RNA synthesis
2294 Cordycepin
RNA synthesis inhibitor
2483 Costunolide
Inhibitor of human telomerase activity
0970 Cycloheximide
Inhibitor of protein synthesis
4520 Cytarabine
Nucleoside analog; inhibits DNA replication
3917 Daptomycin
Antibiotic; inhibits protein, DNA and RNA synthesis in gram-positive bacteria
1467 Daunorubicin hydrochloride
RNA synthesis inhibitor
3857 Dexrazoxane hydrochloride
Topoisomerase II inhibitor
3260 Epirubicin hydrochloride
Inhibits DNA synthesis and function. Inhibits DNA topoisomerase II
4659 Floxuridine
Disrupts DNA replication; inhibits thymidylate synthetase
3495 Fludarabine
Purine analog; inhibits DNA synthesis
3257 5-Fluorouracil
Inhibits RNA and DNA synthesis
4131 G418 disulfate salt
Aminoglycoside antibiotic; used in cell culture
3849 Gatifloxacin
Antibiotic; inhibits bacterial type II topoisomerase
3259 Gemcitabine hydrochloride
DNA synthesis inhibitor
1416 Homoharringtonine
Inhibitor of protein synthesis. Antileukemic agent
5740 6-Hydroxy-DL-DOPA
Allosteric inhibitor of RAD52; also APE1 inhibitor
6068 Lin28 1632
RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor
3765 Linezolid
Antibiotic; inhibits protein synthesis in gram-positive bacteria
4103 6-Mercaptopurine
Purine analog; inhibits DNA and RNA synthesis
1489 Mithramycin A
Inhibitor of DNA and RNA polymerase
3258 Mitomycin C
Inhibitor of DNA synthesis
5340 NSC 617145
Werner syndrome helicase (WRN) inhibitor
2623 Oxaliplatin
Inhibitor of DNA synthesis
5419 PCNA I1
PCNA inhibitor
6070 Pladienolide B
mRNA splicing inhibitor; antitumor
4089 Puromycin dihydrochloride
Protein synthesis inhibitor
6168 RI 1
RAD51 recombinase inhibitor
4501 Ribavirin
Antiviral guanosine analog; blocks eIF4E activity
4121 Rifampicin
Antibiotic; inhibits bacterial RNA polymerase
4723 T2AA
PCNA inhibitor
3267 Thiostrepton
Antibiotic, inhibits protein synthesis
3253 Triptolide
Inhibits RNAPII-mediated transcription; antitumor, anti-inflammatory and immunosuppressive
3863 Trovafloxacin mesylate
Antibiotic; inhibits bacterial DNA synthesis
3787 Viomycin
Antibiotic; inhibits bacterial DNA synthesis
4067 YK 4-279
Inhibitor of RNA helicase A (RHA)

Other

Cat No Product Name / Activity
5962 3-AP
Ribonucleotide reductase inhibitor; also an iron chelator
4099 Azathioprine
Purine analog; prodrug of 6-mercaptopurine (Cat. No. 4103)
3771 Azithromycin
Antibiotic; inhibits 50S ribosomal subunit formation and elongation at transpeptidation
3681 Bendamustine hydrochloride
Cytostatic agent; exhibits DNA alkylating and purine analog properties
5015 5-BrdU
Synthetic thymidine analog; used for cell proliferation assays
4091 Cyclophosphamide
Alkylating agent; chemotherapeutic
3357 Ellipticine
Antitumor and intercalating agent; inhibits DNA topoisomerase II
5284 trans-ISRIB
Integrated stress response (ISR) inhibitor
5761 RG 102240
Gene switch ligand for use in induceable gene expression systems
5810 RS 1
High affinity RAD51 activator; also CRISPR enhancer
4990 Stavudine
Nucleoside analog; antiviral
4460 Trifluorothymidine
Thymidylate synthase inhibitor; induces DNA fragmentation

Related Targets

    RNA, DNA and protein synthesis are fundamental processes necessary for all life.

    RNA synthesis (transcription) begins by uncoiling a section of DNA that will be used as the template. RNA polymerase binds to a promoter sequence and initiates separation of the DNA double helix. Complementary ribonucleotides align and RNA polymerase catalyzes their polymerization. The newly synthesized RNA strand undergoes post-transcriptional processing before it leaves the nucleus.

    Protein synthesis (translation) is the process of synthesizing a polypeptide chain from a mRNA template. Initiation factors trigger the small ribosomal subunit to bind to mRNA, allowing a tRNA molecule (which contains an amino acid) with an anticodon complementary to the mRNA initiation codon to bind. The large ribosomal subunit now binds and translation occurs. tRNA molecules sequentially bind to the complementary mRNA sequences and ribosomal enzymes catalyze the formation of peptide (amide) bonds.

    DNA synthesis is a semiconservative process; each strand serves as a template for the synthesis of a new one. Helicase enzymes 'unwind' the DNA double helix, allowing DNA polymerase access to the polynucleotide chain. DNA polymerase cannot initiate de novo DNA synthesis - an RNA primer is required - and synthesis occurs in a 5' to 3' direction. DNA polymerases have 'proofreading' ability, which is essential to prevent mutations in the genome.

    Literature for DNA, RNA and Protein Synthesis

    Cancer

    Cancer Research Product Guide

    A collection of over 750 products for cancer research, the guide includes research tools for the study of:

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