Home / Cell Biology / Cell Metabolism / DNA, RNA and Protein Synthesis / DNA, RNA and Protein Synthesis Inhibitors / Fludarabine

Fludarabine

Cat. No. 3495 2 Citations 1 Review Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 3495 - Fludarabine | C10H12FN5O4 | CAS No. 21679-14-1
Description: Purine analog; inhibits DNA synthesis
Chemical Name: 9-β-D-Arabinofuranosyl-2-fluoro-9H-purin-6-amine
Purity: ≥99% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Purine analog that inhibits DNA synthesis. Exhibits antiproliferative activity (IC50 = 1.54 μM in RPMI cells) and triggers apoptosis through increasing Bax and decreasing Bid, XIAP and survivin expression. Inhibits cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in lymphocytes. Also displays anticancer activity against hematological malignancies in vivo.

Compound Libraries

Fludarabine is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 285.23
Formula C10H12FN5O4
Storage Desiccate at +4°C
Purity ≥99% (HPLC)
CAS Number 21679-14-1
PubChem ID 657237
InChI Key HBUBKKRHXORPQB-FJFJXFQQSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N3C2=NC(F)=NC(N)=C2N=C3)[C@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.52 100

Preparing Stock Solutions

The following data is based on the product molecular weight 285.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.51 mL 17.53 mL 35.06 mL
5 mM 0.7 mL 3.51 mL 7.01 mL
10 mM 0.35 mL 1.75 mL 3.51 mL
50 mM 0.07 mL 0.35 mL 0.7 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

Bellosillo et al (1999) In vitro evaluation of fludar. in combination with cyclophosph. and/or mitoxan. in B-cell chronic lymphocytic leukemia. Blood 94 2836 PMID: 10515887

Meng et al (2007) Antitumor activity of fludar. against human multiple myeloma in vitro and in vivo. Eur.J.Haematol. 79 486 PMID: 17976186

Torella et al (2007) Fludarabine prevents smooth muscle proliferation in vitro and neointimal hyperplasia in vivo through specific inhibition of STAT-1 activation. Am.J.Physiol.Heart Circ.Physiol. 292 H2935 PMID: 17293493

Frank et al (1999) Fludarabine-induced immunosuppression is associated with inhibition of STAT1 signaling. Nat.Med. 5 444 PMID: 10202937

If you know of a relevant reference for Fludarabine, please let us know.

View Related Products by Target

View Related Products by Product Action

View all DNA, RNA and Protein Synthesis Inhibitors

Keywords: Fludarabine, Fludarabine supplier, Purine, analog, inhibits, inhibitors, DNA, synthesis, Proapoptotic, increases, Bax, decreases, Bid, XIAP, survivin, expression, Apoptosis, Inducer, STAT1, chemotherapeutics, DNA,, RNA, and, Protein, Synthesis, Inducers, STAT, 3495, Tocris Bioscience

2 Citations for Fludarabine

Citations are publications that use Tocris products. Selected citations for Fludarabine include:

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703

Odendall et al (2014) Diverse intracellular pathogens activate type III IF. expression from peroxisomes. Nat Immunol 15 717 PMID: 24952503

Do you know of a great paper that uses Fludarabine from Tocris? Please let us know.

Reviews for Fludarabine

Average Rating: 5 (Based on 1 Review.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Fludarabine?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


STAT1 inhibitor in IFN beta signaling.
By Richa Rani on 01/04/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Hepatic stellate cells and hepatocytes

I used it to check its effect on drug stimulated hepatic stellate cell and its signaling.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis