Fludarabine

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Cat.No. 3495 - Fludarabine | C10H12FN5O4 | CAS No. 21679-14-1
Description: Purine analog; inhibits DNA synthesis
Chemical Name: 9-β-D-Arabinofuranosyl-2-fluoro-9H-purin-6-amine
Purity: ≥99% (HPLC)
Datasheet
Citations (2)
Reviews (1)
Literature

Biological Activity

Purine analog that inhibits DNA synthesis. Exhibits antiproliferative activity (IC50 = 1.54 μM in RPMI cells) and triggers apoptosis through increasing Bax and decreasing Bid, XIAP and survivin expression. Inhibits cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in lymphocytes. Also displays anticancer activity against hematological malignancies in vivo.

Compound Libraries

Fludarabine is also offered as part of the Tocriscreen Plus and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 285.23
Formula C10H12FN5O4
Storage Desiccate at +4°C
Purity ≥99% (HPLC)
CAS Number 21679-14-1
PubChem ID 657237
InChI Key HBUBKKRHXORPQB-FJFJXFQQSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N3C2=NC(F)=NC(N)=C2N=C3)[C@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.52 100

Preparing Stock Solutions

The following data is based on the product molecular weight 285.23. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.51 mL 17.53 mL 35.06 mL
5 mM 0.7 mL 3.51 mL 7.01 mL
10 mM 0.35 mL 1.75 mL 3.51 mL
50 mM 0.07 mL 0.35 mL 0.7 mL

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References

References are publications that support the biological activity of the product.

Bellosillo et al (1999) In vitro evaluation of fludarabine in combination with cyclophosphamide and/or mitoxantrone in B-cell chronic lymphocytic leukemia. Blood 94 2836 PMID: 10515887

Meng et al (2007) Antitumor activity of fludarabine against human multiple myeloma in vitro and in vivo. Eur.J.Haematol. 79 486 PMID: 17976186

Torella et al (2007) Fludarabine prevents smooth muscle proliferation in vitro and neointimal hyperplasia in vivo through specific inhibition of STAT-1 activation. Am.J.Physiol.Heart Circ.Physiol. 292 H2935 PMID: 17293493

Frank et al (1999) Fludarabine-induced immunosuppression is associated with inhibition of STAT1 signaling. Nat.Med. 5 444 PMID: 10202937


If you know of a relevant reference for Fludarabine, please let us know.

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View all DNA, RNA and Protein Synthesis Inhibitors

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2 Citations for Fludarabine

Citations are publications that use Tocris products. Selected citations for Fludarabine include:

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703

Odendall et al (2014) Diverse intracellular pathogens activate type III interferon expression from peroxisomes. Nat Immunol 15 717 PMID: 24952503


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Reviews for Fludarabine

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STAT1 inhibitor in IFN beta signaling
By Richa Rani on 01/04/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Hepatic stellate cells and hepatocytes

I used it to check its effect on drug stimulated hepatic stellate cell and its signaling.

review image

Literature in this Area

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Cancer

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