Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewFludarabine
Description: Purine analog; inhibits DNA synthesis
Chemical Name: 9-β-D-Arabinofuranosyl-2-fluoro-9H-purin-6-amine
Purity: ≥99% (HPLC)
Biological Activity for Fludarabine
Fludarabine is a purine analog that inhibits DNA synthesis. Exhibits antiproliferative activity (IC50 = 1.54 μM in RPMI cells) and triggers apoptosis through increasing Bax and decreasing Bid, XIAP and survivin expression. Inhibits cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in lymphocytes. Also displays anticancer activity against hematological malignancies in vivo.
Compound Libraries for Fludarabine
Fludarabine is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.
Technical Data for Fludarabine
| M. Wt | 285.23 |
| Formula | C10H12FN5O4 |
| Storage | Desiccate at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 21679-14-1 |
| PubChem ID | 657237 |
| InChI Key | HBUBKKRHXORPQB-FJFJXFQQSA-N |
| Smiles | O[C@@H]1[C@@H](CO)O[C@@H](N3C2=NC(F)=NC(N)=C2N=C3)[C@H]1O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Fludarabine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.52 | 100 |
Preparing Stock Solutions for Fludarabine
The following data is based on the product molecular weight 285.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.51 mL | 17.53 mL | 35.06 mL |
| 5 mM | 0.7 mL | 3.51 mL | 7.01 mL |
| 10 mM | 0.35 mL | 1.75 mL | 3.51 mL |
| 50 mM | 0.07 mL | 0.35 mL | 0.7 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Fludarabine
References for Fludarabine
References are publications that support the biological activity of the product.
Bellosillo et al (1999) In vitro evaluation of fludar. in combination with cyclophosph. and/or mitoxan. in B-cell chronic lymphocytic leukemia. Blood 94 2836 PMID: 10515887
Meng et al (2007) Antitumor activity of fludar. against human multiple myeloma in vitro and in vivo. Eur.J.Haematol. 79 486 PMID: 17976186
Torella et al (2007) Fludarabine prevents smooth muscle proliferation in vitro and neointimal hyperplasia in vivo through specific inhibition of STAT-1 activation. Am.J.Physiol.Heart Circ.Physiol. 292 H2935 PMID: 17293493
Frank et al (1999) Fludarabine-induced immunosuppression is associated with inhibition of STAT1 signaling. Nat.Med. 5 444 PMID: 10202937
If you know of a relevant reference for Fludarabine, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Fludarabine, Fludarabine supplier, Purine, analog, inhibits, inhibitors, DNA, synthesis, Proapoptotic, increases, Bax, decreases, Bid, XIAP, survivin, expression, Apoptosis, Inducer, STAT1, chemotherapeutics, DNA,, RNA, and, Protein, Synthesis, Inducers, STAT, 3495, Tocris Bioscience
2 Citations for Fludarabine
Citations are publications that use Tocris products. Selected citations for Fludarabine include:
Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703
Odendall et al (2014) Diverse intracellular pathogens activate type III IF. expression from peroxisomes. Nat Immunol 15 717 PMID: 24952503
Do you know of a great paper that uses Fludarabine from Tocris? Please let us know.
Reviews for Fludarabine
Average Rating: 5 (Based on 1 Review.)
Have you used Fludarabine?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
STAT1 inhibitor in IFN beta signaling.
By
Richa Rani on 01/04/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Hepatic stellate cells and hepatocytes
I used it to check its effect on drug stimulated hepatic stellate cell and its signaling.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cancer Research Product Guide
A collection of over 750 products for cancer research, the guide includes research tools for the study of:
- Cancer Metabolism
- Epigenetics in Cancer
- Receptor Signaling
- Cell Cycle and DNA Damage Repair
- Angiogenesis
- Invasion and Metastasis
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 170 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product GuideNew
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Excitotoxicity
- Stem Cells