Fluorescence Imaging

Tocris offers a broad range of fluorescent and bioluminescent reagents, to facilitate the visualization of sub-cellular components in live and fixed cells with high sensitivity and selectivity. The product range includes fluorescent probes, dyes and indicators.

Literature (1)

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    Spectra Viewer

    Use this spectra viewer to interactively view the excitation and emission spectra for our fluorescent dyes (including Janelia Fluor®, TFAX, Cyanine and BDY dyes). Spectra for other dyes are also included for reference purposes. This is a useful tool for planning and designing experiments, particularly for assessing multiplexing options. Note: absorption spectra may be displayed instead of excitation spectra.

    Characteristics of Fluorescent Compounds

    Fluorescent compounds are generally small organic molecules (although they may also be peptides or proteins) that absorb light radiation of a specific wavelength and emit radiation at a longer wavelength (lower energy). The compounds cease to emit radiation immediately, when the incident radiation stops. Fluorescent compounds have a maximum excitation and emission wavelength, measured in nanometers, and the difference between the two is known as the Stokes shift. The ratio of the number of photons emitted to the number absorbed is an indicator of the efficiency of the fluorescence process and is known as the quantum yield. How strongly a fluorescent compound absorbs radiation is known as the extinction coefficient, and links the amount of radiation of a given wavelength absorbed to the molar concentration of the compound. Another useful parameter to take into account is the lifetime, which corresponds to the average time (measured in picoseconds) that the compound spends in an excited state before emitting a photon and returning to the ground state.

    Featured Product

    New and Exclusive Fluorescent Microtubule Probe

    Released in October 2019, Taxol Janelia Fluor® 646 Cat. No. 6266 is a fluorogenic microtubule probe that 'switches on' only once bound to microtubules, enabling hassle-free, no-wash experiments. Simply apply to live or fixed cells and leave to incubate for an hour before viewing on a standard confocal microscope.

    Labeling cells with the Taxol Janelia Fluor® 646 probe avoids the disruptive and time-consuming washing step required with most microtubule probes. This product is particularly beneficial for those working on cells with adhesion sensitivity, providing a convenient way to visualize cells with minimal disruption. Taxol Janelia Fluor® 646 is also ideal for tracking microtubules in live cell experiments.

    Application of Taxol Janelia Fluor® 646 dye on COS7 cells
    Taxol Janelia Fluor® 646 Dye-labeled COS7 cells

    Application of Taxol Janelia Fluor® 646 on COS7 cells; 3 µM Taxol Janelia Fluor® 646 was used to label COS7 cells for one hour at 37°C. Images were taken on the Leica TCS SP8 Confocal Laser Scanning Microscope.

    All images very kindly provided by Prof. Christian Soeller and his team, from the University of Exeter; acquired by Evelina Lucinskaite, Anna Meletiou and Alexander Clowsley.

    Literature for Fluorescence Imaging

    Tocris offers the following scientific literature for Fluorescence Imaging to showcase our products. We invite you to request* your copy today!

    *Please note that Tocris will only send literature to established scientific business / institute addresses.

    Fluorescent Probes and Dyes

    Fluorescent Probes and Dyes Research Product Guide

    This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

    • Fluorescent Dyes
    • Dyes for Flow Cytometry
    • Fluorescent Probes
    • Anti-fade Reagents
    • Bioluminescent Substrates
    • Fluorogenic Dyes for Light-Up Aptamers
    • Fluorescent Probes for Imaging Bacteria
    • TSA Reagents for Enhancing IHC, ICC & FISH Signals