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NAI

Cat. No. 7004 Submit a Review Datasheet / COA / SDS

Discontinued Product

7004 has been discontinued.

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Description: Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Datasheets References

Biological Activity for NAI

NAI is a reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data for NAI

M. Wt 187.2
Formula C10H9N3O
Storage Store at -20°C
CAS Number 1055970-47-2
PubChem ID 66563654
InChI Key OWQPEDNXDCVXJO-UHFFFAOYSA-N
Smiles CC1=C(C(N2C=CN=C2)=O)C=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for NAI

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References for NAI

References are publications that support the biological activity of the product.

Lee et al (2017) Comparison of SHAPE reagents for mapping RNA structures inside living cells. RNA 23 169 PMID: 27879433

Kwok et al (2013) Determination of in vivo RNA structure in low-abundance transcripts. Nat.Commun. 4 2971 PMID: 24336128

Spitale et al (2013) RNA SHAPE analysis in living cells. Nat.Chem.Biol. 9 18 PMID: 23178934

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Keywords: NAI, NAI supplier, Reagent, RNA, SHAPE, MaP, chemistry, experiments, 1methyl7nitroisatoic, mapping, secondary, tertiary, structures, cell, permeable, in, vivo, DNA,, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 7004, Tocris Bioscience

Citations for NAI

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Currently there are no citations for NAI.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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