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Description: Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone
Literature (2)

Biological Activity for NAI

NAI is a reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data for NAI

M. Wt 187.2
Formula C10H9N3O
Storage Store at -20°C
CAS Number 1055970-47-2
PubChem ID 66563654
Smiles CC1=C(C(N2C=CN=C2)=O)C=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NAI

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 374.4 2000

Preparing Stock Solutions for NAI

The following data is based on the product molecular weight 187.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
20 mM 0.27 mL 1.34 mL 2.67 mL
100 mM 0.05 mL 0.27 mL 0.53 mL
200 mM 0.03 mL 0.13 mL 0.27 mL
1000 mM 0.01 mL 0.03 mL 0.05 mL

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Product Datasheets for NAI

Certificate of Analysis / Product Datasheet
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References for NAI

References are publications that support the biological activity of the product.

Lee et al (2017) Comparison of SHAPE reagents for mapping RNA structures inside living cells. RNA 23 169 PMID: 27879433

Kwok et al (2013) Determination of in vivo RNA structure in low-abundance transcripts. Nat.Commun. 4 2971 PMID: 24336128

Spitale et al (2013) RNA SHAPE analysis in living cells. Nat.Chem.Biol. 9 18 PMID: 23178934

If you know of a relevant reference for NAI, please let us know.

Keywords: NAI, NAI supplier, Reagent, RNA, SHAPE, MaP, chemistry, experiments, 1methyl7nitroisatoic, mapping, secondary, tertiary, structures, cell, permeable, in, vivo, DNA,, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 7004, Tocris Bioscience

Citations for NAI

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