NAI

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Cat.No. 7004 - NAI | C10H9N3O | CAS No. 1055970-47-2
Description: Reagent for RNA SHAPE-MaP experiments in vivo; cell permeable
Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Reagent for RNA SHAPE-MaP experiments in live cells. Acylates the 2'-OH in RNA, labels single stranded or flexible regions more than folded regions (SHAPE methodology). Enables analysis of RNA secondary structures at single nucleotide resolution. Exhibits greater signals with lower background than 1M7 for in vivo measurement of the RNA structurome. Also more stable than 1M7 in water. β-mercaptoethanol terminates reaction. Cell permeable.

Technical Data

M. Wt 187.2
Formula C10H9N3O
Storage Store at -20°C
CAS Number 1055970-47-2
PubChem ID 66563654
InChI Key OWQPEDNXDCVXJO-UHFFFAOYSA-N
Smiles CC1=C(C(N2C=CN=C2)=O)C=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.72 100

Preparing Stock Solutions

The following data is based on the product molecular weight 187.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.34 mL 26.71 mL 53.42 mL
5 mM 1.07 mL 5.34 mL 10.68 mL
10 mM 0.53 mL 2.67 mL 5.34 mL
50 mM 0.11 mL 0.53 mL 1.07 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Lee et al (2017) Comparison of SHAPE reagents for mapping RNA structures inside living cells. RNA 23 169 PMID: 27879433

Kwok et al (2013) Determination of in vivo RNA structure in low-abundance transcripts. Nat.Commun. 4 2971 PMID: 24336128

Spitale et al (2013) RNA SHAPE analysis in living cells. Nat.Chem.Biol. 9 18 PMID: 23178934


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Keywords: NAI, NAI supplier, Reagent, RNA, SHAPE, MaP, chemistry, experiments, 1methyl7nitroisatoic, mapping, secondary, tertiary, structures, cell, permeable, in, vivo, DNA,, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 7004, Tocris Bioscience

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