Puromycin dihydrochloride

Pricing Availability   Qty
Cat.No. 4089 - Puromycin dihydrochloride | C22H29N7O5.2HCl | CAS No. 58-58-2
Description: Protein synthesis inhibitor
Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Purity: ≥98% (HPLC)
Citations (8)
Reviews (1)
Literature (2)

Biological Activity for Puromycin dihydrochloride

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Compound Libraries for Puromycin dihydrochloride

Puromycin dihydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Puromycin dihydrochloride

M. Wt 544.43
Formula C22H29N7O5.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 58-58-2
PubChem ID 443311
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Puromycin dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 54.44 100
DMSO 54.44 100

Preparing Stock Solutions for Puromycin dihydrochloride

The following data is based on the product molecular weight 544.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.18 mL 18.37 mL
5 mM 0.37 mL 1.84 mL 3.67 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Puromycin dihydrochloride

Certificate of Analysis / Product Datasheet
Select another batch:

References for Puromycin dihydrochloride

References are publications that support the biological activity of the product.

Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141

Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866

If you know of a relevant reference for Puromycin dihydrochloride, please let us know.

View Related Products by Product Action

View all DNA, RNA and Protein Synthesis Inhibitors

Keywords: Puromycin dihydrochloride, Puromycin dihydrochloride supplier, Puromycin, antibiotics, protein, synthesis, inhibitors, inhibits, translation, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4089, Tocris Bioscience

8 Citations for Puromycin dihydrochloride

Citations are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:

Dobrikov et al (2014) Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol 34 439 PMID: 24248602

Dasgupta et al (2014) Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells. PLoS One 9 e103604 PMID: 25079948

Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407

Yu et al (2019) Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. PLoS One 14 e0210608 PMID: 30625226

Do you know of a great paper that uses Puromycin dihydrochloride from Tocris? Please let us know.

Reviews for Puromycin dihydrochloride

Average Rating: 4 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Puromycin dihydrochloride?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells..
By Niroj Shrestha on 01/20/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.

Puromycin should be stored in aliquots at -20 degree C.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

A collection of over 170 products for cell cycle and DNA damage research, the guide includes research tools for :

  • Cell Cycle and Mitosis
  • DNA Damage Repair
  • Targeted Protein Degradation
  • Ubiquitin Proteasome Pathway
  • Chemotherapy Targets