Puromycin dihydrochloride

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Cat.No. 4089 - Puromycin dihydrochloride | C22H29N7O5.2HCl | CAS No. 58-58-2
Description: Protein synthesis inhibitor
Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Technical Data

M. Wt 544.43
Formula C22H29N7O5.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 58-58-2
PubChem ID 443311
InChI Key MKSVFGKWZLUTTO-FZFAUISWSA-N
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 54.44 100
water 54.44 100

Preparing Stock Solutions

The following data is based on the product molecular weight 544.43. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.18 mL 18.37 mL
5 mM 0.37 mL 1.84 mL 3.67 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141

Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866 PMID:


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View all DNA, RNA and Protein Synthesis Inhibitors

Keywords: Puromycin antibiotics protein synthesis inhibitors inhibits translation DNA, RNA and Protein Synthesis

1 Citation for Puromycin dihydrochloride

Citations are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:

Zhu et al (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. J Neurosci 10 e0143255 PMID: 26571119


Do you know of a great paper that uses Puromycin dihydrochloride from Tocris? If so please let us know.

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

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