Puromycin dihydrochloride

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Description: Protein synthesis inhibitor
Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (16)
Reviews (1)
Literature (2)

Biological Activity for Puromycin dihydrochloride

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Technical Data for Puromycin dihydrochloride

M. Wt 544.43
Formula C22H29N7O5.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 58-58-2
PubChem ID 443311
InChI Key MKSVFGKWZLUTTO-FZFAUISWSA-N
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Puromycin dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 54.44 100
DMSO 54.44 100

Preparing Stock Solutions for Puromycin dihydrochloride

The following data is based on the product molecular weight 544.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.18 mL 18.37 mL
5 mM 0.37 mL 1.84 mL 3.67 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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Product Datasheets for Puromycin dihydrochloride

Certificate of Analysis / Product Datasheet
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References for Puromycin dihydrochloride

References are publications that support the biological activity of the product.

Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141

Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866 PMID: 4590173


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View all DNA, RNA and Protein Synthesis Inhibitors

Keywords: Puromycin dihydrochloride, Puromycin dihydrochloride supplier, Puromycin, antibiotics, protein, synthesis, inhibitors, inhibits, translation, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4089, Tocris Bioscience

16 Citations for Puromycin dihydrochloride

Citations are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:

Yu et al (2019) Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. PLoS One 14 e0210608 PMID: 30625226

Zhu et al (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. J Neurosci 10 e0143255 PMID: 26571119

Dobrikov et al (2014) Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol 34 439 PMID: 24248602

Dasgupta et al (2014) Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells. PLoS One 9 e103604 PMID: 25079948

Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407

Luke A et al (2016) Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Elife 5 PMID: 27661255

Dasgupta et al (2015) Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease. PLoS One 10 e0145333 PMID: 26691307

Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33453847

Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33082139

Mark L et al (2020) Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation. Cell Rep 31 107785 PMID: 32579917

Philipp et al (2020) An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells. Cancers (Basel) 12 PMID: 32580291

Leung et al (2016) WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143 398 PMID: 26839343

Tao et al (2021) CT45A1 promotes the metastasis of osteosarcoma cells in vitro and in vivo through β-catenin. Cell Death Dis 12 650 PMID: 34172717

Balaji M et al (2021) Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J Biol Chem 296 100386 PMID: 33556374

Nabil G et al (2021) PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs. Stem Cell Reports 16 2958-2972 PMID: 34739847

Ma et al (2017) AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma. Am J Transl Res. 9 507 PMID: 28337279


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Reviews for Puromycin dihydrochloride

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Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells..
By Niroj Shrestha on 01/20/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.

Puromycin should be stored in aliquots at -20 degree C.

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Literature in this Area

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