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Biological Activity for Puromycin dihydrochloride
Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.
Compound Libraries for Puromycin dihydrochloride
Technical Data for Puromycin dihydrochloride
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Puromycin dihydrochloride
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Puromycin dihydrochloride
The following data is based on the product molecular weight 544.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.84 mL||9.18 mL||18.37 mL|
|5 mM||0.37 mL||1.84 mL||3.67 mL|
|10 mM||0.18 mL||0.92 mL||1.84 mL|
|50 mM||0.04 mL||0.18 mL||0.37 mL|
Product Datasheets for Puromycin dihydrochloride
References for Puromycin dihydrochloride
References are publications that support the biological activity of the product.
Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141
Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866
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8 Citations for Puromycin dihydrochloride
Citations are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:
Dobrikov et al (2014) Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol 34 439 PMID: 24248602
Dasgupta et al (2014) Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells. PLoS One 9 e103604 PMID: 25079948
Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407
Yu et al (2019) Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. PLoS One 14 e0210608 PMID: 30625226
Leung et al (2016) WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143 398 PMID: 26839343
Dasgupta et al (2015) Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease. PLoS One 10 e0145333 PMID: 26691307
Zhu et al (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. J Neurosci 10 e0143255 PMID: 26571119
Ma et al (2017) AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma. Am J Transl Res. 9 507 PMID: 28337279
Do you know of a great paper that uses Puromycin dihydrochloride from Tocris? Please let us know.
Reviews for Puromycin dihydrochloride
Average Rating: 4 (Based on 1 Review.)
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Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.
Puromycin should be stored in aliquots at -20 degree C.
Literature in this Area
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