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Description: Reagent for RNA SHAPE-MaP chemistry
Alternative Names: 1-Methyl-7-nitroisatoic anhydride
Chemical Name: 1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione
Purity: ≥95% (HPLC)
Literature (2)

Biological Activity for 1M7

1M7 is a reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Technical Data for 1M7

M. Wt 222.15
Formula C9H6N2O5
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 73043-80-8
PubChem ID 12535373
Smiles CN1C(OC(C2=CC=C([N+]([O-])=O)C=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 1M7

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 22.21 100

Preparing Stock Solutions for 1M7

The following data is based on the product molecular weight 222.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.5 mL 22.51 mL 45.01 mL
5 mM 0.9 mL 4.5 mL 9 mL
10 mM 0.45 mL 2.25 mL 4.5 mL
50 mM 0.09 mL 0.45 mL 0.9 mL

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Product Datasheets for 1M7

Certificate of Analysis / Product Datasheet
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References for 1M7

References are publications that support the biological activity of the product.

Mortimer et al (2007) A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 129 4144 PMID: 17367143

Turner et al (2013) Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 19 1857 PMID: 24141619

Siegfried et al (2014) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 11 959 PMID: 25028896

Smola et al (2015) Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 54 6867 PMID: 26544910

If you know of a relevant reference for 1M7, please let us know.

Keywords: 1M7, 1M7 supplier, Reagent, RNA, SHAPE, MaP, chemistry, experiments, 1methyl7nitroisatoic, mapping, secondary, tertiary, structures, 1-methyl-7-nitroisatoic, anhydride, DNA,, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 6602, Tocris Bioscience

Citations for 1M7

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Currently there are no citations for 1M7. Do you know of a great paper that uses 1M7 from Tocris? Please let us know.

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Literature in this Area

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