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Description: Reagent for RNA SHAPE-MaP chemistry
Alternative Names: 1-Methyl-7-nitroisatoic anhydride
Chemical Name: 1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione
Purity: ≥95% (HPLC)
Biological Activity
Reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.
Technical Data
| M. Wt | 222.15 |
| Formula | C9H6N2O5 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 73043-80-8 |
| PubChem ID | 12535373 |
| InChI Key | MULNCJWAVSDEKJ-UHFFFAOYSA-N |
| Smiles | CN1C(OC(C2=CC=C([N+]([O-])=O)C=C12)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.21 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 222.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.5 mL | 22.51 mL | 45.01 mL |
| 5 mM | 0.9 mL | 4.5 mL | 9 mL |
| 10 mM | 0.45 mL | 2.25 mL | 4.5 mL |
| 50 mM | 0.09 mL | 0.45 mL | 0.9 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Mortimer et al (2007) A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 129 4144 PMID: 17367143
Turner et al (2013) Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 19 1857 PMID: 24141619
Siegfried et al (2014) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 11 959 PMID: 25028896
Smola et al (2015) Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 54 6867 PMID: 26544910
If you know of a relevant reference for 1M7, please let us know.
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