1M7

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Cat.No. 6602 - 1M7 | C9H6N2O5 | CAS No. 73043-80-8
Description: Reagent for RNA SHAPE-MaP chemistry
Alternative Names: 1-Methyl-7-nitroisatoic anhydride
Chemical Name: 1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Reagent for RNA SHAPE-MaP experiments. Enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Technical Data

M. Wt 222.15
Formula C9H6N2O5
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 73043-80-8
PubChem ID 12535373
InChI Key MULNCJWAVSDEKJ-UHFFFAOYSA-N
Smiles CN1C(OC(C2=CC=C([N+]([O-])=O)C=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.21 100

Preparing Stock Solutions

The following data is based on the product molecular weight 222.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.5 mL 22.51 mL 45.01 mL
5 mM 0.9 mL 4.5 mL 9 mL
10 mM 0.45 mL 2.25 mL 4.5 mL
50 mM 0.09 mL 0.45 mL 0.9 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Mortimer et al (2007) A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 129 4144 PMID: 17367143

Turner et al (2013) Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 19 1857 PMID: 24141619

Siegfried et al (2014) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 11 959 PMID: 25028896

Smola et al (2015) Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 54 6867 PMID: 26544910


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Citations for 1M7

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