Viral Transduction Enhancers

Viral transduction is the process of introducing foreign genetic material into a cell with a virus or viral vector. This method is a common tool used by researchers to enable stable introduction of a gene into the genome of a target cell or cells. Small molecules can be used to enhance the viral transduction process and increase target gene expression.

Products
Background
Literature (1)
Cat. No. Product Name / Activity
1101 Cyclosporin A
Calcineurin inhibitor; also inhibits MPTP
6982 Cyclosporin H
Viral transduction enhancer
1467 Daunorubicin hydrochloride
RNA synthesis inhibitor
4027 16,16-Dimethyl Prostaglandin E2
Synthetic prostaglandin E2 (Cat. No. 2296) derivative
1226 Etoposide
Topoisomerase II inhibitor
4821 PF 03814735
Aurora kinase A and B inhibitor
2296 Prostaglandin E2
Endogenous prostanoid
1292 Rapamycin
mTOR inhibitor; immunosuppressant
4652 SAHA
Class I and II HDAC inhibitor
1285 Staurosporine
Non-selective protein kinase inhibitor

Viral transduction is a common process used by researchers for a range of processes, including but not limited to transgene expression, gene silencing, site-directed gene editing, generation of transgenic animals, expression of fluorescent proteins for imaging, induction of pluripotent stem cells, and stem cell reprogramming and differentiation.

Viral vectors, or viral particles, used to deliver genetic material into a cell are first made in a packaging cell line, commonly HEK293 cells. A plasmid containing the genetic material to be transferred is inserted into the packaging cell line, along with plasmids that code viral genes for reverse transcriptase, integrase and the viral capsid. The packaging cell line produces viral vectors that replicate within cells, which are then harvested and used to transduce target cells.

There are three main types of virus used for viral transduction; retroviruses, lentiviruses and adeno-associated viruses. Retroviruses are stable and replication-defective, meaning they don't contain sequences required by the virus to make proteins for viral replication and infection. However, they can only transfect cells that are actively dividing and so are unsuitable for use with cells that are post-mitotic, such as neurons. Lentiviruses are a subtype of retroviruses that can transfect non-dividing cells. Genetic material transfected in lentiviruses is integrated into the host cell genome and the viral vector remains in the cell, being passed to its progeny when the cell divides. Adeno-associated viruses also incorporate into the host cell genome and can infect both dividing and non-dividing cells.

Small molecules can be used to enhance viral transduction. For example, prostaglandin E2 (Cat. No. 2296) improves lentiviral transduction and increases viral vector copy numbers resulting in increased transgene expression in CD34+ hematopoietic stem and progenitor cells.

Literature for Viral Transduction Enhancers

Tocris offers the following scientific literature for Viral Transduction Enhancers to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


New Product Guide

New Product Guide [Spring/Summer 2019]

Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • PROTACs
  • Signal Transduction
  • Stem Cells