Doxycycline hyclate

Pricing Availability   Qty
Description: Broad-spectrum MMP inhibitor; tetracycline derivative
Chemical Name: (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate
Purity: ≥92% (HPLC)
Citations (9)
Reviews (1)
Protocols (2)
Literature (2)

Biological Activity for Doxycycline hyclate

Doxycycline hyclate is a broad-spectrum antibiotic and MMP inhibitor; tetracycline derivative. Doxycycline hyclate increases and promotes smooth muscle cell adhesion; inhibits proliferation and migration. Protects the microvasculature by inhibiting plasminogen systems.

For more information about how Doxycycline hyclate may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Reprogramming MEFs into Skeletal Muscle Progenitors.

Technical Data for Doxycycline hyclate

M. Wt 512.94
Formula C22H24N2O8.HCl.0.5C2H6O.0.5H2O
Storage Store at +4°C
Purity ≥92% (HPLC)
CAS Number 24390-14-5
PubChem ID 54686183
Smiles OC1=C(C(C2=C(O)[C@@](C(C(C(N)=O)=C(O)[C@H]4N(C)C)=O)(O)[C@@]4([H])[C@@H](O)[C@@]([H])2[C@H]3C)=O)C3=CC=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Doxycycline hyclate

Solvent Max Conc. mg/mL Max Conc. mM
water 48.09 100
DMSO 48.09 100

Preparing Stock Solutions for Doxycycline hyclate

The following data is based on the product molecular weight 512.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.95 mL 9.75 mL 19.5 mL
5 mM 0.39 mL 1.95 mL 3.9 mL
10 mM 0.19 mL 0.97 mL 1.95 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

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Product Datasheets for Doxycycline hyclate

Certificate of Analysis / Product Datasheet
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References for Doxycycline hyclate

References are publications that support the biological activity of the product.

Franco et al (2006) Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices. Am.J.Pathol. 168 1697 PMID: 16651635

Burggraf et al (2007) Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia. Neurobiol.Dis. 25 506 PMID: 17166729

Wernig et al (2008) A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nat.Biotechnol. 26 916 PMID: 18594521

If you know of a relevant reference for Doxycycline hyclate, please let us know.

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Keywords: Doxycycline hyclate, Doxycycline hyclate supplier, tetracycline, antibiotics, inhibits, MMPs, matrix, metalloprotease, metalloproteinase, inhibitors, Matrix, Metalloprotease, Antibiotics, 4090, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Doxycycline hyclate (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to

9 Citations for Doxycycline hyclate

Citations are publications that use Tocris products. Selected citations for Doxycycline hyclate include:

Ruchira et al (2021) 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell 28 846-862.e8 PMID: 33784497

Enrique et al (2021) Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Nat Commun 12 3499 PMID: 34108480

Balaji M et al (2021) Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J Biol Chem 296 100386 PMID: 33556374

Ling et al (2021) Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell 28 1090-1104.e6 PMID: 33915081

Do you know of a great paper that uses Doxycycline hyclate from Tocris? Please let us know.

Reviews for Doxycycline hyclate

Average Rating: 4 (Based on 1 Review.)

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Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa.
By Anonymous on 02/09/2019
Species: Human

Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa. E coli was used as a standard to compare.

review image

Protocols for Doxycycline hyclate

The following protocols feature additional information for the use of Doxycycline hyclate (Cat. No. 4090).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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