Doxycycline hyclate

Pricing Availability   Qty
Description: Broad-spectrum MMP inhibitor; tetracycline derivative
Chemical Name: (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate
Purity: ≥92% (HPLC)
Citations (9)
Reviews (1)
Protocols (2)
Literature (1)

Biological Activity for Doxycycline hyclate

Doxycycline hyclate is a broad-spectrum antibiotic and MMP inhibitor; tetracycline derivative. Doxycycline hyclate increases and promotes smooth muscle cell adhesion; inhibits proliferation and migration. Protects the microvasculature by inhibiting plasminogen systems.

For more information about how Doxycycline hyclate may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Reprogramming MEFs into Skeletal Muscle Progenitors.

Technical Data for Doxycycline hyclate

M. Wt 512.94
Formula C22H24N2O8.HCl.0.5C2H6O.0.5H2O
Storage Store at +4°C
Purity ≥92% (HPLC)
CAS Number 24390-14-5
PubChem ID 54686183
Smiles OC1=C(C(C2=C(O)[C@@](C(C(C(N)=O)=C(O)[C@H]4N(C)C)=O)(O)[C@@]4([H])[C@@H](O)[C@@]([H])2[C@H]3C)=O)C3=CC=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Doxycycline hyclate

Solvent Max Conc. mg/mL Max Conc. mM
water 48.09 100
DMSO 48.09 100

Preparing Stock Solutions for Doxycycline hyclate

The following data is based on the product molecular weight 512.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.95 mL 9.75 mL 19.5 mL
5 mM 0.39 mL 1.95 mL 3.9 mL
10 mM 0.19 mL 0.97 mL 1.95 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

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Product Datasheets for Doxycycline hyclate

Certificate of Analysis / Product Datasheet
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References for Doxycycline hyclate

References are publications that support the biological activity of the product.

Franco et al (2006) Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices. Am.J.Pathol. 168 1697 PMID: 16651635

Burggraf et al (2007) Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia. Neurobiol.Dis. 25 506 PMID: 17166729

Wernig et al (2008) A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nat.Biotechnol. 26 916 PMID: 18594521

If you know of a relevant reference for Doxycycline hyclate, please let us know.

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Keywords: Doxycycline hyclate, Doxycycline hyclate supplier, tetracycline, antibiotics, inhibits, MMPs, matrix, metalloprotease, metalloproteinase, inhibitors, Matrix, Metalloprotease, Antibiotics, 4090, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Doxycycline hyclate (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to

9 Citations for Doxycycline hyclate

Citations are publications that use Tocris products. Selected citations for Doxycycline hyclate include:

Connor H et al (2022) Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. Elife 11 PMID: 35678392

Mitinori et al (2022) Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells. EMBO J 41 e109457 PMID: 35603814

Sato et al (2019) Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells. Stem Cell Reports 13 352 PMID: 31353225

Rukhsana et al (2020) An Update of the Virion Proteome of Kaposi Sarcoma-Associated Herpesvirus. Viruses 12 PMID: 33276600

Do you know of a great paper that uses Doxycycline hyclate from Tocris? Please let us know.

Reviews for Doxycycline hyclate

Average Rating: 4 (Based on 1 Review.)

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Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa.
By Anonymous on 02/09/2019
Species: Human

Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa. E coli was used as a standard to compare.

review image

Protocols for Doxycycline hyclate

The following protocols feature additional information for the use of Doxycycline hyclate (Cat. No. 4090).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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Angiogenesis in Cancer Poster

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