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Description: Reagent for RNA SHAPE-MaP experiments in live cells
Chemical Name: 1H-Imidazol-1-yl(2-methyl-3-furanyl)methanone
Literature (2)

Biological Activity for FAI

FAI is a reagent for RNA SHAPE-MaP experiments in live cells. Can be used to map RNA structures in vivo by forming stable 2'-OH adducts with RNA, blocking reverse transcriptase elongation. In vitro, FAI activity can be quenched with Dithiothreitol (Cat. No. 3154) and can be used to probe the secondary structure of mouse embryonic stem cell 5S rRNA. At elevated temperatures, FAI forms more stable adducts and exhibits less hydrolysis than NAI (Cat. No 7004).

Technical Data for FAI

M. Wt 176.17
Formula C9H8N2O2
Storage Store at -20°C
CAS Number 1415238-77-5
PubChem ID 66563653
Smiles O=C(C=1C=COC1C)N2C=NC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FAI

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 17.62 100

Preparing Stock Solutions for FAI

The following data is based on the product molecular weight 176.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.68 mL 28.38 mL 56.76 mL
5 mM 1.14 mL 5.68 mL 11.35 mL
10 mM 0.57 mL 2.84 mL 5.68 mL
50 mM 0.11 mL 0.57 mL 1.14 mL

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References for FAI

References are publications that support the biological activity of the product.

Chan et al (2017) Comparative analysis reveals furoyl in vivo selective hydroxyl acylation analyzed by primer extension reagents form stable ribosyl ester adducts. Biochemistry 56 18111 PMID: 28319368

Spitale et al (2013) RNA SHAPE analysis in living cells. Nat.Chem.Biol. 9 18 PMID: 23178934

If you know of a relevant reference for FAI, please let us know.

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Citations for FAI

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Literature in this Area

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