Scientific Meeting Posters
A range of scientific meeting posters authored by in-house experts
Written by our in-house experts and external collaborators, the following range of posters have been presented at scientific meetings, and provide an informative summary of key areas of research. We invite you to request your copy today.
Principles and Perspectives on Design and Chemical Space
Maple, H et al.This poster describes the generation of a database of Degraders (PROTACs®) from the literature. The Degraders were profiled according to the constituent ligands, linker type, linker length and physicochemical properties and this information was used to establish a set of guidelines for the design and synthesis of cell-permeable Degrader molecules. Presented at the 20th SCI/RSC Medicinal Chemistry Symposium 2019, Cambridge, UK.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
Enabling Research By Provision of Chemical Tools
Developing New Chemical Tools for Biological Research
Maple, H et al.The Tocris chemistry team has considerable chemistry knowledge and skill which it has recently been applying to the generation of new chemical probes from known tools and compounds to help answer biological questions. This poster presents some of the work carried out by Tocris scientists and focuses on two areas: the generation of libraries of chemical building blocks to support Degrader (PROTAC) research and the development of a fluorescent probe to study integrin biology. Presented at Chemical Tools for Complex Biological Systems II, 2019, Janelia Research Campus, USA.
Novel Live-cell and Fixable Mitochondrial Probes
MitoBrilliant™ Dyes For Flow Cytometry and Imaging
Wood, P et al.Fluorescent probes that accumulate in the mitochondria are useful tools to explore mitochondrial function and cell health. This poster reports the development inhouse and characterization of novel fluorescent probes, known as MitoBrilliant that specifically localize to mitochondria due to mitochondrial membrane potential. The probes are suitable for live or fixed cell staining. The data demonstrate the specificity and enhanced properties of our MitoBrilliant dyes and their use in a range of applications. This poster was presented at CYTO 2022, June 2022, Philadelphia, USA.
Targeted Protein Degradation
Generating a Chemical Toolbox to Support PROTAC R&D
Maple, H et al.
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia
Validating Targets for TPD Using dTAG
A Comprehensive Workflow Solution
Amrollahi, P et al.The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple WesternTM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.