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Submit Reviewtrans-ISRIB
Description: Integrated stress response (ISR) inhibitor
Chemical Name: N,N'-trans-1,4-cyclohexanediylbis[2-(4-chlorophenoxy)acetamide]
Purity: ≥99% (HPLC)
Biological Activity for trans-ISRIB
trans-ISRIB is an integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC50 = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.
Technical Data for trans-ISRIB
| M. Wt | 451.34 |
| Formula | C22H24Cl2N2O4 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 1597403-47-8 |
| PubChem ID | 1011240 |
| InChI Key | HJGMCDHQPXTGAV-UHFFFAOYSA-N |
| Smiles | O=C(COC1=CC=C(Cl)C=C1)N[C@@H]2CC[C@@H](NC(COC3=CC=C(Cl)C=C3)=O)CC2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for trans-ISRIB
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.51 | 10 with gentle warming |
Preparing Stock Solutions for trans-ISRIB
The following data is based on the product molecular weight 451.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 22.16 mL | 110.78 mL | 221.56 mL |
| 0.5 mM | 4.43 mL | 22.16 mL | 44.31 mL |
| 1 mM | 2.22 mL | 11.08 mL | 22.16 mL |
| 5 mM | 0.44 mL | 2.22 mL | 4.43 mL |
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Product Datasheets for trans-ISRIB
References for trans-ISRIB
References are publications that support the biological activity of the product.
Sidrauski et al (2013) Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2 e00498 PMID: 23741617
Sekine et al (2015) Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound. Science 348 1027 PMID: 25858979
Hinnebusch (2015) Cell biology. Blocking stress response for better memory? Science 348 967 PMID: 26023119
Chen et al (2021) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
If you know of a relevant reference for trans-ISRIB, please let us know.
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Keywords: trans-ISRIB, trans-ISRIB supplier, Potent, inhibitors, inhibits, of, PERK, signaling, UPR, unfolded, protein, response, endoplasmic, reticulum, ER, stress, memory, consolidation, eIF2, alpha, kinases, ISR, integrated, ISRIB, stem, cell, maintenance, self, renewal, pluripotent, CEPT, differentiated, cryo, DNA,, RNA, and, Protein, Synthesis, Miscellaneous, Compounds, Other, stress/UPR, Stem, Cell, Proliferation, Cocktail, 5284, Tocris Bioscience
3 Citations for trans-ISRIB
Citations are publications that use Tocris products. Selected citations for trans-ISRIB include:
Tristan et al (2021) Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Rep. 16 3076 PMID: 34861164
Chen et al (2019) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep. 21 2895 PMID: 29212034
Do you know of a great paper that uses trans-ISRIB from Tocris? Please let us know.
Reviews for trans-ISRIB
Average Rating: 5 (Based on 2 Reviews.)
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Inhibits integrated stress response in HEK293 cells.
By
mandi gandelman on 05/01/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
Used trans-ISRIB to inhibit the integrated stress response (ISR) in HEK293 cells. Induced the ISR and added ISRIB (100 and 500nM) overnight. Evaluated p-eif2a (unchanged) and cleaved caspase 3 (increased in a dose-dependent manner with ISRIB.
High specific inhibitor.
By
Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: cortical neurons
Toxicity assay. 500 nM concentration was used. Highly effective Integrated stress response (ISR) inhibitor. Works very well.
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