Indisulam

Pricing Availability   Qty
Cat.No. 6782 - Indisulam | C14H12ClN3O4S2 | CAS No. 165668-41-7
Description: Molecular glue; pre-mRNA splicing modulator
Chemical Name: N1-(3-Chloro-1H-indol-7-yl)-1,4-benzenedisulfonamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (3)

Biological Activity

Acts as a molecular glue to induce proteosomal degradation of mRNA splicing factor RBM39 (also designated CAPERα, HCC1, FSAP59, and RNPC2), via binding to DCAF15. Acts as a pre-mRNA splicing modulator (SPLAMs; splicing inhibitor sulfonamides), causes aberrant pre-mRNA splicing. Suppresses proliferation of cancer cell lines. Reduces viability of HCT-116 cells (IC50 = 0.56 μM). Induces cell cycle arrest in the G1 phase in cancer cell lines. Also a high affinity carbonic anhydrase isozyme XII (hCA XII) inhibitor (Ki = 3.0-5.7 nM).

Technical Data

M. Wt 385.84
Formula C14H12ClN3O4S2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 165668-41-7
PubChem ID 216468
InChI Key SETFNECMODOHTO-UHFFFAOYSA-N
Smiles NS(=O)(C1=CC=C(S(=O)(NC2=CC=CC3=C2NC=C3Cl)=O)C=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.58 100

Preparing Stock Solutions

The following data is based on the product molecular weight 385.84. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.59 mL 12.96 mL 25.92 mL
5 mM 0.52 mL 2.59 mL 5.18 mL
10 mM 0.26 mL 1.3 mL 2.59 mL
50 mM 0.05 mL 0.26 mL 0.52 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

Han et al (2017) Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science 28 356 PMID: 28302793

Uehara et al (2017) Selective degradation of splicing factor CAPERa by anticancer sulfonamides. Nat.Chem.Biol. 13 675 PMID: 28437394

Ozawa et al (2001) E7070, a novel sulphonamide agent with potent antitumour activity in vitro and in vivo. Eur.J.Cancer. 37 2275 PMID: 11677118

Owa et al (1999) Discovery of novel antitumor sulfonamides targeting G1 phase of the cell cycle. J.Med.Chem. 42 3789 PMID: 10508428

Di et al (2018) Function, clinical application, and strategies of Pre-mRNA splicing in cancer. Cell Death Differ. doi: 10.1038/s41418- PMID: 30464224

Vullo et al (2005) Carbonic anhydrase inhibitors. Inhibition of the transmembrane isozyme XII with sulfonamides-a new target for the design of antitumor and antiglaucoma drugs? Bioorg.Med.Chem.Lett. 15 963 PMID: 15686894


If you know of a relevant reference for Indisulam, please let us know.

Keywords: Indisulam, Indisulam supplier, High, affinity, carbonic, anhydrase, isozyme, XII, (hCA, XII), inhibitors, inhibits, SPLAMs, splicing, inhibitor, sulfonamides, RNAbinding, protein, 39, RBM39, antiglaucoma, Pre-mRNA, modulator, molecular, glue, Carbonic, anhydrases, DNA,, RNA, and, Protein, Synthesis, Ubiquitin, E3, Ligases, Biochemicals, Molecular, Biology, Cell, Cycle, Inhibitors, 6782, Tocris Bioscience

Citations for Indisulam

Citations are publications that use Tocris products.

Currently there are no citations for Indisulam. Do you know of a great paper that uses Indisulam from Tocris? Please let us know.

Reviews for Indisulam

There are currently no reviews for this product. Be the first to review Indisulam and earn rewards!

Have you used Indisulam?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Targeted Protein Degradation

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders (e.g. PROTACs)
  • Degrader Building Blocks
  • Custom Degrader Services
  • UPS Proteins and Assays
  • Assays for Protein Degradation
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Targeted Protein Degradation

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia