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Description: Modulator of alternative polyadenylation; inhibits rod photoreceptor gene expression via Nr2e3
Chemical Name: 6,7-Dimethoxy-2-[((4-phenyl-3,6-dihydropyridin-1(2H)-yl)methyl)]quinazolin-4(3H)-one
Purity: ≥98% (HPLC)
Literature (2)

Biological Activity for T4

T4 is a modulator of alternative polyadenylation (APA) (IC50 values are 2.1 μM and 8.5 μM in U2OS and 293T cells, respectively). T4 promotes distal-to-proximal APA usage in multiple transcripts, using distinctive A-rich motifs through autoregulated PABPN1 signaling. T4 exhibits no inhibitory activity against a panel of 414 kinases. T4 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa. T4 inhibits rod photoreceptor gene expression via Nr2e3 in a luciferase-based assay (IC50 = 0.07 μM).

Technical Data for T4

M. Wt 377.44
Formula C22H23N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 785708-33-0
PubChem ID 135415476
Smiles COC1=C(OC)C=C2C(C(NC(CN3CCC(C4=CC=CC=C4)=CC3)=N2)=O)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for T4

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 0.75 2

Preparing Stock Solutions for T4

The following data is based on the product molecular weight 377.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 132.47 mL 662.36 mL 1324.71 mL
0.1 mM 26.49 mL 132.47 mL 264.94 mL
0.2 mM 13.25 mL 66.24 mL 132.47 mL
1 mM 2.65 mL 13.25 mL 26.49 mL

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References for T4

References are publications that support the biological activity of the product.

Nakamura et al (2017) Small molecule photoregulin3 prevents retinal degeneration in the Rho P23H mouse model of retinitis pigmentosa. ELife 6 PMID: 29148976

Araki et al (2018) Decoding transcriptome dynamics of genome-encoded polyadenylation and autoregulation with small-molecule modulators of alternative polyadenylation. Cell.Chem.Biol. 25 1470 PMID: 30293940

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Keywords: T4, T4 supplier, T4, photoregulin3, alternative, polyadenylation, polyadenylators, polyadenylate, RNA, APA, modulators, modulates, DNA,, and, Protein, Synthesis, 7054, Tocris Bioscience

Citations for T4

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