5-BrdU

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Cat.No. 5015 - 5-BrdU | C9H11BrN2O5 | CAS No. 59-14-3
Description: Synthetic thymidine analog; used for cell proliferation assays
Chemical Name: 5-Bromo-2-deoxyuridine
Purity: ≥99% (HPLC)
Datasheet
Citations
Reviews (1)
Literature

Biological Activity

Synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation.

Technical Data

M. Wt 307.1
Formula C9H11BrN2O5
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 59-14-3
PubChem ID 6035
InChI Key WOVKYSAHUYNSMH-RRKCRQDMSA-N
Smiles O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 15.36 50
DMSO 30.71 100

Preparing Stock Solutions

The following data is based on the product molecular weight 307.1. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.28 mL 32.56 mL
5 mM 0.65 mL 3.26 mL 6.51 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Stacey and Hitomi (2008) Cell cycle studies basd upon quantitative image analysis. Cytometry A 73A 270 PMID: 18163464

Barker et al (2013) A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary. PLoS One 8 e63692 PMID: 23691086


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Keywords: 5-BrdU, 5-BrdU supplier, 5-BrdU, DNA, synthesis, replication, cell, proliferation, assays, label, thymidine, analog, analogue, DNA,, RNA, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 5015, Tocris Bioscience

Citations for 5-BrdU

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Reviews for 5-BrdU

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Proliferation marker for studying neurogenesis
By Luiz Alexandre Magno on 07/17/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.

PMID: 17020783
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