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Description: Synthetic thymidine analog; used for cell proliferation assays
Chemical Name: 5-Bromo-2-deoxyuridine
Purity: ≥99% (HPLC)
Citations (1)
Reviews (1)

Biological Activity for 5-BrdU

5-BrdU is a synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.

Compound Libraries for 5-BrdU

5-BrdU is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 5-BrdU

M. Wt 307.1
Formula C9H11BrN2O5
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 59-14-3
PubChem ID 6035
Smiles O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 5-BrdU

Solvent Max Conc. mg/mL Max Conc. mM
water 15.36 50
DMSO 30.71 100

Preparing Stock Solutions for 5-BrdU

The following data is based on the product molecular weight 307.1. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.28 mL 32.56 mL
5 mM 0.65 mL 3.26 mL 6.51 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

Molarity Calculator

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References for 5-BrdU

References are publications that support the biological activity of the product.

Stacey and Hitomi (2008) Cell cycle studies basd upon quantitative image analysis. Cytometry A 73A 270 PMID: 18163464

Barker et al (2013) A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary. PLoS One 8 e63692 PMID: 23691086

Long et al (2015) Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells. Cell Res. 25 1171 PMID: 26251165

If you know of a relevant reference for 5-BrdU, please let us know.

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1 Citation for 5-BrdU

Citations are publications that use Tocris products. Selected citations for 5-BrdU include:

Muhammad Abid et al (2022) Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features. Cell Death Dis 13 670 PMID: 35915082

Do you know of a great paper that uses 5-BrdU from Tocris? Please let us know.

Reviews for 5-BrdU

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Proliferation marker for studying neurogenesis.
By Luiz Alexandre Magno on 07/17/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.

PMID: 17020783
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