You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Biological Activity for Pladienolide B
Pladienolide B is a mRNA splicing inhibitor that decreases splicing capacity up to 75% in vitro. Pladienolide B directly targets splicesome-associated 130 (SAP130), inhibits splicing factor 3B subunit (SF3B1) and impairs U2 small nuclear ribonucleoprotein (U2 snRNP) interaction with pre-mRNA. Pladienolide B arrests the cell cycle in G1 and G2/M phases and displays antitumor activity against gastric cancer cells (IC50 values are 1.6-4.9 nM). Cell permeable and active in vivo.
Technical Data for Pladienolide B
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Pladienolide B
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Pladienolide B
The following data is based on the product molecular weight 536.7. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.02 mM||93.16 mL||465.81 mL||931.62 mL|
|0.1 mM||18.63 mL||93.16 mL||186.32 mL|
|0.2 mM||9.32 mL||46.58 mL||93.16 mL|
|1 mM||1.86 mL||9.32 mL||18.63 mL|
References for Pladienolide B
References are publications that support the biological activity of the product.
Lee et al (2016) Therapeutic targeting of splicing in cancer. Nature Medicine 22 976
Pederiva et al (2016) Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. 23 1648 PMID: 27315300
Sato et al (2014) High antitumor activity of pladienolide B and its derivative in gastric cancer. Cancer Sci. 105 110 PMID: 24635824
If you know of a relevant reference for Pladienolide B, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Pladienolide B, Pladienolide B supplier, mRNA, splicing, inhibitors, inhibits, splicesome-associated, 130, SAP130, factor, 3B, subunit, SF3B1, U2, small, nuclear, ribonucleoprotein, snRNP, cell, cycle, antitumor, anticancer, permeable, active, in, vivo, DNA,, RNA, and, Protein, Synthesis, Cell, Cycle, Inhibitors, 6070, Tocris Bioscience
9 Citations for Pladienolide B
Citations are publications that use Tocris products. Selected citations for Pladienolide B include:
Kotake et al (2007) Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat.Chem.Biol. 3 570 PMID: 17643112
Frank et al (2021) Modulation of SF3B1 in the pre-mRNA spliceosome induces a RIG-I-dependent type I IFN response. J Biol Chem 297 101277 PMID: 34619148
Jing et al (2021) Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Cell 184 4032-4047.e31 PMID: 34171309
Disney et al (2008) Short-circuiting RNA splicing. Nat.Chem.Biol. 4 723 PMID: 19008883
Wolf et al (2022) 8C-like cells capture the human zygotic genome activation program in vitro. Cell Stem Cell 29 449-459.e6 PMID: 35216671
Lee et al (2020) ATR Protects the Genome against R Loops through a MUS81-Triggered Feedback Loop. Mol Cell 77 514-527.e4 PMID: 31708417
Wenjing et al (2023) Highly efficient generation of blastocyst-like structures from spliceosomes-repressed mouse totipotent blastomere-like cells. Sci China Life Sci 66 423-435 PMID: 36633710
Crews et al (2016) RNA splicing modulation selectively impairs leukemia stem cell maintenance in secondary human AML. Cell stem cell 19 599 PMID: 27570067
Salton et al (2016) Small molecule modulators of pre-mRNA splicing in cancer therapy. Trends Mol.Med. 22 28 PMID: 26700537
Do you know of a great paper that uses Pladienolide B from Tocris? Please let us know.
Reviews for Pladienolide B
There are currently no reviews for this product. Be the first to review Pladienolide B and earn rewards!
Have you used Pladienolide B?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 170 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product Guide
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Stem Cells