Stem Cells

Stem cells are unspecialized cells that are capable of self-renewal through mitotic cell division, even after long periods of inactivity. Stem cells may be induced to form more specialized cells of a tissue or organ by a process termed cellular differentiation, which is defined by the potency of a cell.

Targets
Literature (4)
Pathways (3)

Owing to their regenerative potential, stem cells in some tissues and organs are utilized to repair or replace old or damaged cells and this property may be useful in regenerative medicine. In other tissues such as the heart, this does not occur and stem cells only divide under strict conditions.

The four main types of stem cells are: embryonic stem cells (ESCs) found in the inner cell mass of blastocysts; adult (or somatic) stem cells, found in adult tissues and responsible for maintenance and repopulation of specific cell types; induced pluripotent stem cells (iPSCs), produced by the reprogramming of somatic cells; and cancer stem cells (CSCs).

Stem cells offer the potential for treating many diseases including cancer and neurodegenerative conditions, such as Parkinson's disease or Huntington's disease, or nerve damage due to trauma.


Resources for Stem Cell Research

Stem Cell Protocols

Our protocol snapshots highlight how small molecules may be used in the stem cell workflow for maintenance, reprogramming and differentiation.

Browse Protocols
Transdifferentiating Fibroblasts into Neurons Protocol

Blog Post: Cryopreservation of Stem Cells with the ROCK Inhibitor

Cryopreservation for storage of cells is a key step in stem cell protocols. The ROCK inhibitor Y-27632 improves post-thaw survival rates. This blog post outlines how this compound protects stem cells from dissociated-associated apoptosis.

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ROCK inhibitor for cryopreservation of stem cells

Blog Post: GMP Ancillary Materials for Stem Cell Therapy Manufacture

Stem cell therapies have the potential to treat several diseases, and these therapies are now entering clinical trials and approaching the clinic. This blog discusses small molecules as an ancillary material (AMs) for stem cell therapy manufacture, including their advantages over other types of AMs.

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Blog Post - GMP ancillary meterials for stem cell therapy manufacture

Blog Post: Small Molecules in Stem Cell Research

Small molecules are increasingly being used alone, or in combination with growth factors, to modulate stem cells. They can be used at all stages of the stem cell workflow, i.e. in reprogramming, maintenance, storage and differentiation protocols. This blog post outlines the advantages of using small molecules.

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Five reasons to use small molecules in stem cell research

Literature for Stem Cells

Tocris offers the following scientific literature for Stem Cells to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Stem Cell

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • Regenerative Medicine
Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cell Workflow

Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.

Pathways for Stem Cells

Notch

Notch Signaling Pathway

The Notch pathway is involved in determination of cell fate, regulation of pattern formation and other developmental settings. Disrupted signaling can cause developmental defects and a range of adult pathologies.
TGF-β

TGF-β Signaling Pathway

The TGF-β signaling pathway is involved in the regulation of growth and proliferation of cells along with migration, differentiation and apoptosis.
Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.