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Description: Thymidylate synthetase inhibitor
Alternative Names: 5-FU
Chemical Name: 5-Fluoro-2,4-(1H,3H)-pyrimidinedione
Purity: ≥99% (HPLC)
Citations (2)
Literature (4)
Pathways (1)

Biological Activity for 5-Fluorouracil

5-Fluorouracil is an anticancer agent. Metabolized to form fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine (FUTP). FdUMP inhibits thymidylate synthase, causing a reduction in dTMP synthesis. FUTP and FdUTP are misincorporated into RNA and DNA respectively.

Technical Data for 5-Fluorouracil

M. Wt 130.08
Formula C4H3FN2O2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 51-21-8
PubChem ID 3385
Smiles O=C1C(F)=CNC(N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 5-Fluorouracil

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 13.01 100
ethanol 1.3 10

Preparing Stock Solutions for 5-Fluorouracil

The following data is based on the product molecular weight 130.08. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 7.69 mL 38.44 mL 76.88 mL
5 mM 1.54 mL 7.69 mL 15.38 mL
10 mM 0.77 mL 3.84 mL 7.69 mL
50 mM 0.15 mL 0.77 mL 1.54 mL

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Product Datasheets for 5-Fluorouracil

Certificate of Analysis / Product Datasheet
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References for 5-Fluorouracil

References are publications that support the biological activity of the product.

Ghoshal and Jacob (1997) An alternative molecular mechanism of action of 5-fluorouracil, a potent anticancer drug. Biochem.Pharmacol. 53 1569 PMID: 9264308

Peters et al (2002) Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim.Biophys.Acta. 1587 194 PMID: 12084461

Longley et al (2003) 5-Fluorouracil: mechanisms of action and clinical strategies. Nat.Rev.Cancer 3 330 PMID: 12724731

If you know of a relevant reference for 5-Fluorouracil, please let us know.

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Keywords: 5-Fluorouracil, 5-Fluorouracil supplier, inhibitors, inhibits, RNA, DNA, synthesis, thymidylate, synthetases, Synthases, antimetabolites, 5-FU, chemotherapeutics, DNA,, and, Protein, Synthesis, Thymidylate, Synthetase, 3257, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Fluorouracil, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

2 Citations for 5-Fluorouracil

Citations are publications that use Tocris products. Selected citations for 5-Fluorouracil include:

Fukushima et al (2015) Atonal homolog 1 protein stabilized by tumor necrosis factor α induces high malignant potential in colon cancer cell line. Cancer Sci 106 1000 PMID: 26017781

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703

Do you know of a great paper that uses 5-Fluorouracil from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Metabolism Research Product Guide

Cancer Metabolism Research Product Guide

This product guide reviews some of the main areas in cancer metabolism research and lists around 150 products that can be used to investigate metabolic pathways in cancer including:

Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:

  • Cell Cycle and Mitosis
  • DNA Damage Repair
  • Targeted Protein Degradation
  • Ubiquitin Proteasome Pathway
  • Chemotherapy Targets
Cancer Metabolism Poster

Cancer Metabolism Poster

This poster summarizes the main metabolic pathways in cancer cells and highlights potential targets for cancer therapeutics. Genetic changes and epigenetic modifications in cancer cells alter the regulation of cellular metabolic pathways providing potential cancer therapeutic targets.

Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for 5-Fluorouracil

Apoptosis Signaling Pathway

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.
p53 Signaling Pathway

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.