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Description: Bivalent SHAPE cross-linking reagent
Alternative Names: trans-bis-isatoic anhydride
Chemical Name: 1,6-Dihydrobenzo[1,2-d:4,5-d']bis([1,3]oxazine)-2,4,7,9-tetraone
Purity: ≥95% (HPLC)
Literature (2)

Biological Activity for TBIA

TBIA (trans-bis-isatoic anhydride) is a bifunctional SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) chemical cross-linking reagent. TBIA has two electrophilic isatoic anhydride moieties, both of which can react with the 2'-OH group in nucleotides adjacent to and distant from the primary RNA sequence. TBIA is used in SHAPE-JuMP (-juxtaposed merged pairs) a technique for exploring RNA tertiary structure proximity on large RNAs, and higher-order RNA-RNA interactions at nanometer resolution.

Technical Data for TBIA

M. Wt 248.15
Formula C10H4N2O6
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C1OC(C2=C(N1)C=C3C(NC(OC3=O)=O)=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TBIA

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.96 20

Preparing Stock Solutions for TBIA

The following data is based on the product molecular weight 248.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 20.15 mL 100.75 mL 201.49 mL
1 mM 4.03 mL 20.15 mL 40.3 mL
2 mM 2.01 mL 10.07 mL 20.15 mL
10 mM 0.4 mL 2.01 mL 4.03 mL

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References for TBIA

References are publications that support the biological activity of the product.

Christy et al (2021) Direct mapping of higher-order RNA interactions by SHAPE-JuMP. Biochemistry 60 1971 PMID: 34121404

If you know of a relevant reference for TBIA, please let us know.

Keywords: TBIA, TBIA supplier, TBIA, trans-bis-isatoic, anhydride, SHAPE-JuMP, bivalent, cross-linker, RNA-RNA, interactions, RNA, tertiary, structure, selective, 2'-hydroxyl, acylation, analyzed, by, primer, extension, and, mutational, profiling, DNA,, Protein, Synthesis, Biochemicals, Molecular, Biology, 7788, Tocris Bioscience

Citations for TBIA

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