Mitomycin C

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Description: DNA cross-linking antitumor agent
Alternative Names: Ametycine
Chemical Name: [1aS-(1aα,8β,8aα,8bα)]-6-Amino-8-[[(aminocarbonyl)oxy]methyl]-1,1a,2,8,8a,8b-hexahydro-8a-methoxy-5-methylazirino[2',3':3,4]pyrrolo[1,2-a]indole-4,7-dione
Purity: ≥98% (HPLC)
Citations (5)
Reviews (1)
Literature (1)
Pathways (1)

Biological Activity for Mitomycin C

Mitomycin C is an antibiotic and antitumor agent. Covalently binds DNA forming intra- and interstrand crosslinks. Inhibits DNA synthesis. Also used for MEF/STO feeder layer preparation in stem cell culture.

Technical Data for Mitomycin C

M. Wt 334.33
Formula C15H18N4O5
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 50-07-7
PubChem ID 5746
Smiles O=C(C(N)=C1C)C2=C(N(C[C@H]4[C@@H]3N4)[C@@]3(OC)[C@@H]2COC(N)=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Mitomycin C

Solvent Max Conc. mg/mL Max Conc. mM
water 0.33 1
DMSO 33.43 100

Preparing Stock Solutions for Mitomycin C

The following data is based on the product molecular weight 334.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.99 mL 14.96 mL 29.91 mL
5 mM 0.6 mL 2.99 mL 5.98 mL
10 mM 0.3 mL 1.5 mL 2.99 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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Product Datasheets for Mitomycin C

Certificate of Analysis / Product Datasheet
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References for Mitomycin C

References are publications that support the biological activity of the product.

Tomasz et al (1987) Isolation and structure of a covalent cross-link adduct between mito. C and DNA. Science 235 1204 PMID: 3103215

Bizanek et al (1992) Isolation and structure of an intrastrand cross-link adduct of mito. C and DNA. Biochemistry 31 3084 PMID: 1554696

Bryja et al (2006) Derivation of mouse embryonic stem cells. Nat.Protoc. 1 2082 PMID: 17487198

Park et al (2016) Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9. Nat.Protoc. 11 2154 PMID: 27711053

If you know of a relevant reference for Mitomycin C, please let us know.

Keywords: Mitomycin C, Mitomycin C supplier, inhibitors, inhibits, DNA, synthesis, cross-linking, antitumor, agents, Apoptosis, Inducer, MitomycinC, antibiotics, chemotherapeutics, stem, cells, feeder, layers, preparation, culture, Ametycine, Inducers, DNA,, RNA, and, Protein, Synthesis, Antibiotics, Stem, Cell, Signaling, 3258, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Mitomycin C, which is known to the State of California to cause cancer. For more information, go to

5 Citations for Mitomycin C

Citations are publications that use Tocris products. Selected citations for Mitomycin C include:

Stepp et al (2018) Molecular basis of MMC enhanced corneal sensory nerve repair after debridement wounding. Sci Rep 8 16960 PMID: 30446696

Kishinevsky et al (2018) HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain DA neurons. Nat Commun 9 4345 PMID: 30341316

Woo et al (2015) Elucidating Compound Mechanism of Action by Network Perturbation Analysis. Cell 162 441 PMID: 26186195

Reinhardt et al (2013) Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. PLoS One 8 e59252 PMID: 23533608

Do you know of a great paper that uses Mitomycin C from Tocris? Please let us know.

Reviews for Mitomycin C

Average Rating: 4 (Based on 1 Review.)

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itotically inactivates mouse embryonic fibroblasts (MEFs) inactivated by Mitomycin treatment.
By Harry Clarke on 02/03/2019
Species: Human

For studying cell cycle arrest Mitomycin has been used. MEFs have been inactivated with Mitomycin treatment.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Programmed Cell Death Poster

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Mitomycin C

Apoptosis Signaling Pathway

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.