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Description: Cell-permeable Zn2+ chelator; also inhibits RNA binding protein Lin28
Chemical Name: N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine
Purity: ≥97% (HPLC)
Citations (3)

Biological Activity for TPEN

TPEN is a heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK1 cells; acts as an intracellular chelator of proteomic Zn2+. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Also inhibits RNA binding protein Lin28 (IC50 = 2.5 μM). Cell permeable.

Technical Data for TPEN

M. Wt 424.54
Formula C26H28N6
Storage Store at +4°C
Purity ≥97% (HPLC)
CAS Number 16858-02-9
PubChem ID 5519

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TPEN

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 10.61 25
ethanol 42.45 100

Preparing Stock Solutions for TPEN

The following data is based on the product molecular weight 424.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.36 mL 11.78 mL 23.55 mL
5 mM 0.47 mL 2.36 mL 4.71 mL
10 mM 0.24 mL 1.18 mL 2.36 mL
50 mM 0.05 mL 0.24 mL 0.47 mL

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Product Datasheets for TPEN

Certificate of Analysis / Product Datasheet
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References for TPEN

References are publications that support the biological activity of the product.

Chimienti et al (2001) Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors. Biochem.Pharmacol. 62 51 PMID: 11377396

Rana et al (2008) Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. J.Inorg.Biochem. 102 489 PMID: 18171589

Matias et al (2010) Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2. J.Fluoresc. 20 377 PMID: 19821015

Hyun et al (2001) Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells. Invest.Opthalmol.Vis.Sci. 42 460

Wang et al (2018) Small-molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28. Cell Rep. 23 3091 PMID: 29874593

If you know of a relevant reference for TPEN, please let us know.

Keywords: TPEN, TPEN supplier, zinc, zn2+, chelating, chelators, cell, permeable, permeant, heavy, metals, Biochemicals, and, Molecular, Biology, DNA,, RNA, Protein, Synthesis, 4309, Tocris Bioscience

3 Citations for TPEN

Citations are publications that use Tocris products. Selected citations for TPEN include:

Tong et al (2022) The Role of Transient Receptor Potential A1 and G Protein-Coupled Receptor 39 in Zinc-Mediated Acute and Chronic Itch in Mice. Front Mol Neurosci 14 768731 PMID: 35095413

Mustafa et al (2023) Curcumin attenuates hydroxychloroquine-mediated apoptosis and oxidative stress via the inhibition of TRPM2 channel signalling pathways in a retinal pigment epithelium cell line. Graefes Arch Clin Exp Ophthalmol PMID: 37099129

Kerns (2018) Zinc ion flux during mammalian sperm capacitation. Nat Commun 9 2061 PMID: 29802294

Do you know of a great paper that uses TPEN from Tocris? Please let us know.

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