TPEN

Pricing Availability Delivery Time Qty
Cat.No. 4309 - TPEN | C26H28N6 | CAS No. 16858-02-9
Description: Cell-permeable Zn2+ chelator
Chemical Name: N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK1 cells; acts as an intracellular chelator of proteomic Zn2+. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Cell permeable.

Technical Data

M. Wt 424.54
Formula C26H28N6
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 16858-02-9
PubChem ID 5519
InChI Key CVRXLMUYFMERMJ-UHFFFAOYSA-N
Smiles N(CC3=CC=CC=N3)(CC4=CC=CC=N4)CCN(CC2=CC=CC=N2)CC1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.61 25
ethanol 42.45 100

Preparing Stock Solutions

The following data is based on the product molecular weight 424.54. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.36 mL 11.78 mL 23.55 mL
5 mM 0.47 mL 2.36 mL 4.71 mL
10 mM 0.24 mL 1.18 mL 2.36 mL
50 mM 0.05 mL 0.24 mL 0.47 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the products' biological activity.

Chimienti et al (2001) Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors. Biochem.Pharmacol. 62 51 PMID: 11377396

Rana et al (2008) Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. J.Inorg.Biochem. 102 489 PMID: 18171589

Matias et al (2010) Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2. J.Fluoresc. 20 377 PMID: 19821015

Hyun et al (2001) Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells. Invest.Opthalmol.Vis.Sci. 42 460 PMID:


If you know of a relevant reference for TPEN, please let us know.

View Related Products by Target

Keywords: TPEN, supplier, zinc, zn2+, chelating, chelators, cell, permeable, permeant, heavy, metals, Biochemicals, and, Molecular, Biology, Tocris Bioscience

Citations for TPEN

Citations are publications that use Tocris products.

Currently there are no citations for TPEN. Do you know of a great paper that uses TPEN from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for TPEN

Protocols

TODO: Add Protocols