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Description: Topoisomerase II inhibitor
Chemical Name: [5R-[5α,5aβ,8aα,9β(R*)]]-9-[(4,6-Ο-Ethylidene-β-D-glucopyranosyl)oxy]-5,8,8a,9-tetrahydro-5-(4-hydroxy-3,5-dimethoxyphenyl)furo[3',4':6,7]naphtho[2,3-d]-1,3-dioxol-6-(5aH)-one
Purity: ≥99% (HPLC)
Citations (4)
Reviews (2)
Literature (2)

Biological Activity for Etoposide

Etoposide is a DNA topoisomerase II inhibitor (IC50 = 59.2 μM). Anticancer. Also enhances adeno-associated virus (AAV) transduction of HeLa cells.

Compound Libraries for Etoposide

Etoposide is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library, Tocriscreen Epigenetics Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Etoposide

M. Wt 588.56
Formula C29H32O13
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 33419-42-0
PubChem ID 36462
Smiles OC(C(OC)=C3)=C(OC)C=[C@@]3[C@@H]2C1=CC6=C(OCO6)C=C1[C@@H](O[C@]4([H])O[C@@](CO[C@@H](C)O7)([H])[C@]7([H])[C@H](O)[C@H]4O)[C@@H]5[C@@H]2[C@](OC5)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Etoposide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 58.86 100

Preparing Stock Solutions for Etoposide

The following data is based on the product molecular weight 588.56. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.7 mL 8.5 mL 16.99 mL
5 mM 0.34 mL 1.7 mL 3.4 mL
10 mM 0.17 mL 0.85 mL 1.7 mL
50 mM 0.03 mL 0.17 mL 0.34 mL

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Product Datasheets for Etoposide

Certificate of Analysis / Product Datasheet
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References for Etoposide

References are publications that support the biological activity of the product.

Burden et al (1996) Toposiomerase II.etoposide interactions direct the formation of drug-induced enzyme-DNA cleavage complexes. J.Biol.Chem. 271 29238 PMID: 8910583

Hande et al (1998) Etoposide: four decades of development of a topoisomerase II inhibitor. Eur.J.Cancer 34 1514 PMID: 9893622

Terada et al (1993) Antitumor agents.3.Synthesis and biological activity of 4β-alkyl derivatives containing hydroxy, amino, and amido groups of 4'-O-demethyl-4-desoxypodophyllotoxin as antitumor agents. J.Med.Chem. 36 1689 PMID: 8389875

Nicolson et al (2016) Identification and validation of small molecules that enhance recombinant adeno-associated virus transduction following high-throughput screens. J.Virol. 90 7019 PMID: 27147738

If you know of a relevant reference for Etoposide, please let us know.

View Related Products by Product Action

View all DNA Topoisomerase Inhibitors

Keywords: Etoposide, Etoposide supplier, Topoisomerase, II, inhibitors, inhibits, DNA, Isomerases, VP16, chemotherapeutics, viral, transduction, inhibitor, Viral, Transduction, Enhancers, 1226, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Etoposide, which is known to the State of California to cause cancer. For more information, go to

4 Citations for Etoposide

Citations are publications that use Tocris products. Selected citations for Etoposide include:

Long et al (2019) Novel peptide dermaseptin-PS1 exhibits anticancer activity via induction of intrinsic apoptosis signalling. J Cell Mol Med 23 1300 PMID: 30461197

Li et al (2013) Transmembrane Protein 214 (TMEM214) mediates endoplasmic reticulum stress-induced caspase 4 enzyme activation and apoptosis. J Biol Chem 288 17908 PMID: 23661706

Nicolson (2014) Recombinant adeno-associated virus utilizes host cell nuclear import machinery to enter the nucleus. J Virol 88 4132 PMID: 24478436

Maswadeh et al (2015) Etoposide incorporated into camel milk phospholipids liposomes shows increased activity against fibrosarcoma in a mouse model. Eur J Cancer 2015 743051 PMID: 25821817

Do you know of a great paper that uses Etoposide from Tocris? Please let us know.

Reviews for Etoposide

Average Rating: 5 (Based on 2 Reviews.)

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Etoposide for inducing DSBs.
By Xiaoyu Zhang on 06/23/2023
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: neuronal cells

Neurons were treated with 1 uM Etoposide to induce DNA double-strand breaks (DSBs), followed by gH2ax staining to detect DSBs.

review image

Etoposide inhibits growth of melanoma cell line at micromolar concentrations.
By Anonymous on 02/07/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: SK-MEL-28

Used for killing assay for in vitro cell culture. Cells were cultured for 24 hours before the addition of the compound into the media at low micromolar concentrations (indicated by the arrow).

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Huntington's Disease Research Product Guide

Huntington's Disease Research Product Guide

This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:

  • Somatic Instability
  • Proteolysis and Inclusion Bodies
  • Transcriptional Dysregulation
  • Mitochondrial Dysfunction
  • Nuclear-Cytoplasmic Transport Interference
  • Excitotoxicity
  • Stem Cells
Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.