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Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols.
Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).
For further information view the product protocol.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble to 100 mg/ml in water|
References are publications that support the biological activity of the product.
Davis et al (2002) Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 97 159 PMID: 12050007
Abe et al (1998) Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 5 708 PMID: 9797877
Shayakhmetov et al (2000) Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 74 10274 PMID: 11044071
Bolis et al (2021) Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 12 7033 PMID: 34857732
Zhao et al (2014) Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 21 e92908 PMID: 24658746
Navarro-Guerrero et al (2021) Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 11 4245 PMID: 33608581
Strack (2022) Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 14 92 PMID: 35062296
Kumar et al (2021) Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2 100874 PMID: 34746857
Joung et al (2022) CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 13 1606 PMID: 35338135
Elegheert et al (2018) Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 13 2991 PMID: 30455477
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The following protocol features additional information for the use of Polybrene (Cat. No. 7711).
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