Potent inhibitor of endoplasmic reticulum associated protein degradation (ERAD). Specifically targets the p97-associated deubiquinating process (PAD) and inhibits ataxin-3 (atx3)-dependent deubiquitination. Also inhibits Sec61-mediated protein translocation at the ER. Displays cytotoxic activity preferentially against cancer cells; induces cell death via the proapoptotic protein NOXA.
|Storage||Desiccate at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|ethanol||3.15||5mM with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 630.44. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.59 mL||7.93 mL||15.86 mL|
|5 mM||0.32 mL||1.59 mL||3.17 mL|
|10 mM||0.16 mL||0.79 mL||1.59 mL|
|50 mM||0.03 mL||0.16 mL||0.32 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Wang et al (2008) Inhibition of p97-dependent protein degradation by eeyarestatin I. J.Biol.Chem. 283 7445 PMID: 18199748
Aletrari et al (2011) Eeyarestatin 1 interferes with both retrograde and anterograde intracellular trafficking pathways. PLoS One 6 e22713 PMID: 21799938
If you know of a relevant reference for Eeyarestatin I, please let us know.
View Related Products by Target
Keywords: Eeyarestatin I, supplier, endoplasmic, reticulum, ER, associated, protein, degradation, ERAD, translocation, inhibitors, inhibits, ER-stress, Translocation,, Exocytosis, &, Endocytosis, Tocris Bioscience
2 Citations for Eeyarestatin I
Citations are publications that use Tocris products. Selected citations for Eeyarestatin I include:
Han et al (2015) Combining valosin-containing protein (VCP) inhibition and suberanilohydroxamic acid (SAHA) treatment additively enhances the folding, trafficking, and function of epilepsy-associated γ-aminobutyric acid, type A (GABAA) receptors. Cell Rep 290 325 PMID: 25406314
Davis et al (2015) Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Biochem Biophys Res Commun 11 1727 PMID: 26074080
Do you know of a great paper that uses Eeyarestatin I from Tocris? If so please let us know.