Y-27632 dihydrochloride

GMP Version Available: Y-27632 dihydrochloride
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Cat.No. 1254 - Y-27632 dihydrochloride | C14H21N3O.2HCl | CAS No. 129830-38-2
Description: Selective p160ROCK inhibitor
Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
Purity: ≥98%
Datasheet
Citations (220)
Reviews (6)
Protocols (5)
Literature (3)

Biological Activity

Selective p160 ROCK inhibitor (Ki values are 0.14, 26, 25 and > 250 μM for p160ROCK, PKC, PKA and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Smooth muscle relaxant and orally active in vivo. Increases survival rate of human embryonic stem (hES) cells and iPSC-BMECs undergoing cryopreservation. Also optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).

Y-27632 synthesized to cGMP guidelines also available.

Compound Libraries

Y-27632 dihydrochloride is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 320.26
Formula C14H21N3O.2HCl
Storage Desiccate at RT
Purity ≥98%
CAS Number 129830-38-2
PubChem ID 14801906
InChI Key VJGZNBYDSDEOED-FJSYBICCSA-N
Smiles N[C@H](C)[C@@]1([H])CC[C@H]([C@](NC2=CC=NC=C2)=O)CC1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 32.03 100

Preparing Stock Solutions

The following data is based on the product molecular weight 320.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Narumiya et al (2000) Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325 273 PMID: 11036610

Uehata et al (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase. Nature 389 990 PMID: 9353125

Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters. 32 516 PMID: 22227712

Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282 PMID: 24172903

Wilson et al (2016) Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition. Tissue.Eng.Part C.Methods 22 1085 PMID: 27846787

Sato et al (2015) SnapShot: Growing Organoids from Stem Cells. Cell 161 1700 PMID: 26091044

Mahe et al (2014) Establishment of gastrointestinal epithelial organoids Curr.Protoc.Mouse.Biol 3 217 PMID: 25105065

Bartfeld et al (2015) In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 148 126 PMID: 25307862

Lancaster et al (2015) Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat. Protoc. 9 2329 PMID: 25188634

Jung et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 17 1225 PMID: 21892181

Wimmer et al (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565 505 PMID: 30651639


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Keywords: Y-27632 dihydrochloride, Y-27632 dihydrochloride supplier, Selective, p160ROCK, inhibitors, inhibits, Rho-Kinase, Rho-Associated, Coiled-Coil, Kinases, ROCK, ROK, stem, cells, organoids, Rho-kinases, Stem, Cell, Proliferation, Organoids, 1254, Tocris Bioscience

220 Citations for Y-27632 dihydrochloride

Citations are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:

Yin et al (2019) Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways. Stem Cell Reports 12 488 PMID: 30745031

Delpiano et al (2019) Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells. Front Pharmacol 9 1462 PMID: 30618754

Wang et al (2019) Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale. PeerJ 6 e6143 PMID: 30643676

Lang et al (2019) Single-Cell Sequencing of iPSC-Da Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes. Cell Stem Cell 24 93 PMID: 30503143

Georgouli et al (2019) Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell 176 757 PMID: 30712866

Larraufie et al (2019) Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery. Cell Rep 26 1399 PMID: 30726726

Vila et al (2019) Quantification of human neuromuscular function through optogenetics. Theranostics 9 1232 PMID: 30867827

Zhuang et al (2019) The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and MetF. cytotoxicity in breast cancer. Cancer Metab 7 1 PMID: 30697423

Martin-Gallausiaux et al (2019) Butyrate Produced by Commensal Bacteria Down-Regulates Indolamine 2,3-Dioxygenase 1 (IDO-1) Expression via a Dual Mechanism in Human Intestinal Epithelial Cells. Front Immunol 9 2838 PMID: 30619249

Ervin et al (2019) Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14. Stem Cell Res Ther 10 43 PMID: 30678718

Park et al (2019) Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction. Nat Commun 10 3123 PMID: 31311935

Benedicto (2017) Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors. Nat Commun 8 15374 PMID: 28524846

Xi (2017) In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Cell Rep 18 1573 PMID: 28178531

Hall (2017) Mesoderm-specific Stat3 deletion affects expression of Sox9 yielding Sox9-dependent phenotypes. PLoS Genet 13 e1006610 PMID: 28166224

Kotton et al (2017) Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells. Cell Stem Cell. 21 472 PMID: 28965766

Tchieu et al (2017) A modular platform for differentiation of human PSCs into all major ectodermal lineages. Cell Stem Cell. 31 399 PMID:  28886367

Shi (2017) The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress. Mol Brain 10 38 PMID: 28800762

Shao (2017) A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nat Commun 8 208 PMID: 28785084

Shelton et al (2014) Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Stem Cell Reports 3 516 PMID: 25241748

Kameoka et al (2014) A high-throughput screen for teratogens using human pluripotent stem cells. J Biomol Screen 137 76 PMID: 24154490

Wrighton et al (2014) Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins. Circ Res 111 18126 PMID: 25422477

Aubdool et al (2014) TRPA1 is essential for the vascular response to environmental cold exposure. Korean J Physiol Pharmacol 5 5732 PMID: 25501034

Kirkeby et al (2013) Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci 6 64 PMID: 23316134

Smith et al (2013) Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues. Mol Cancer Res 11 1159 PMID: 23867565

Calvo et al (2013) Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts. Nat Cell Biol 15 637 PMID: 23708000

Zeller et al (2013) The role of mechanical force and ROS in integrin-dependent signals. PLoS One 8 e64897 PMID: 23738008

Krajcovic et al (2013) mTOR regulates phagosome and entotic vacuole fission. Mol Biol Cell 24 3736 PMID: 24088573

Barthwal et al (2013) Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation. PLoS One 8 e58054 PMID: 23536783

Silva-Santos et al (2009) The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries. J Biol Chem 37 1716 PMID: 19325475

Desai et al (2009) Cell polarity triggered by cell-cell adhesion via E-cadherin. Br J Pharmacol 122 905 PMID: 19258396

Giampieri et al (2009) Localized and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility. Nat Commun 11 1287 PMID: 19838175

Altmann et al (2003) Comparison of signalling mechanisms involved in rat mesenteric microvessel contraction by noradrenaline and sphingosylphosphorylcholine. Br J Pharmacol 138 261 PMID: 12522098

Wibberley et al (2003) Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle. Br J Pharmacol 138 757 PMID: 12642376

Roovers and Assoian (2003) Effects of rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G(1) phase cyclin-dependent kinases. Mol Cell Biol 23 4283 PMID: 12773570

Surks et al (2003) Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. J Biol Chem 278 51484 PMID: 14506264

Hippenstiel et al (2002) Rho protein inactivation induced apoptosis of cultured human endothelial cells. BMC Cell Biol 283 L830 PMID: 12225960

Huang et al (2002) Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate. Crit Care Med 277 27265 PMID: 12011056

Jung et al (2002) Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway. Br J Pharmacol 277 48931 PMID: 12359716

An et al (2002) Stiffness changes in cultured airway smooth muscle cells. Gastroenterology 283 C792 PMID: 12176736

Vinson et al (2001) Myelin-associated glycoprotein interacts with ganglioside GT1b. A mechanism for neurite outgrowth inhibition. J Urol 276 20280 PMID: 11279053

Genga et al (2019) Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell Rep 27 708 PMID: 30995470

Rhee et al (2019) An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons. Cell Rep 27 2212 PMID: 31091457

Meijer et al (2019) A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons. Cell Rep 27 2199 PMID: 31091456

Hackland et al (2019) FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision. Stem Cell Reports 12 920 PMID: 31091435

Celano et al (2019) Identification of Kinases Responsible for p53-Dependent Autophagy. iScience 15 109 PMID: 31048145

Regier et al (2019) User-defined morphogen patterning for directing human cell fate stratification. Sci Rep 9 6433 PMID: 31015521

Ng-Blichfeldt et al (2019) TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation. Am J Physiol Lung Cell Mol Physiol PMID: 30969812

Mair et al (2019) Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell Rep 27 599 PMID: 30970261

Nguyen et al (2019) Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells. Stem Cells Int 2019 3298432 PMID: 31191670

Ranganathan et al (2019) Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model. Infect Immun 87 PMID: 30642900

Bertero et al (2019) Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory. Nat Commun 10 1538 PMID: 30948719

Gao et al (2019) Establishment of porcine and human expanded potential stem cells. Nat Cell Biol 21 687 PMID: 31160711

Davies et al (2015) The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures. PLoS One 10 e0138531 PMID: 26414184

Madsen et al (2015) Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis. EMBO Rep 16 1394 PMID: 26323721

Chen et al (2015) Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. PLoS One 10 e0142554 PMID: 26565809

Romero et al (2015) A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. Elife 4 e07103 PMID: 26102527

Edri et al (2015) Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6 6500 PMID: 25799239

Serra et al (2015) SimV. Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition. PLoS One 10 e0133875 PMID: 26207907

Gjorevski et al (2015) Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Sci Rep 5 11458 PMID: 26165921

Piao et al (2015) Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation. Cell Stem Cell 16 198 PMID: 25658373

Lee et al (2015) Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs. Sci Rep 5 11817 PMID: 26139150

Keimpema et al (2014) GABAergic terminals are a source of galanin to modulate cholinergic neuron development in the neonatal forebrain. Cereb Cortex 24 3277 PMID: 23897649

Moon et al (2014) Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol 7 818 PMID: 24220295

Orgaz et al (2014) Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nat Commun 5 4255 PMID: 24963846

McNary et al (2014) Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton. Tissue Eng Part A 20 921 PMID: 24116978

Williams et al (2014) An in vivo role for Rho kinase activation in the tumour vascular disrupting activity of combretastatin A-4 3-O-phosphate. Br J Pharmacol 171 4902 PMID: 24930520

Huang et al (2014) Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat Biotechnol 32 84 PMID: 24291815

Sun et al (2014) Induction of entosis by epithelial cadherin expression. Cell Res 24 1288 PMID: 25342558

Liu and Chen (2014) Cryopreservation of human pluripotent stem cells in defined medium. Curr Protoc Stem Cell Biol 31 1C.17.1 PMID: 25366897

Luo et al (2014) Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease. Curr Protoc Stem Cell Biol 29 5A.7.1 PMID: 24838915

Mich et al (2014) Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. Elife 3 e02669 PMID: 24843006

Pavlov et al (2014) Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption. J Biol Chem 289 28651 PMID: 25164814

Wile et al (2014) Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs. Nat Protoc 9 2411 PMID: 25232937

Sun et al (2014) Competition between human cells by entosis. Cell Res 24 1299 PMID: 25342560

Lin et al (2013) Activation of β 1 but not β 3 integrin increases cell traction forces. J Neurosci 587 763 PMID: 23395612

Lazzeroni et al (2013) A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation. Exp Neurol 18 984 PMID: 23562876

Pol et al (2013) Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate. Stem Cell Res 247 694 PMID: 23507034

Crompton et al (2013) Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling. Nat Cell Biol 11 1206 PMID: 24013066

Lu et al (2013) Generation of integration-free and region-specific neural progenitors from primate fibroblasts. Curr Biol 3 1580 PMID: 23643533

Hannan et al (2013) Inhibition of Rho-kinase improves erectile function, increases nitric oxide signaling and decreases penile apoptosis in a rat model of cavernous nerve injury. Am J Physiol Lung Cell Mol Physiol 189 1155 PMID: 23021998

Hutchins et al (2013) Calcium release-dependent actin flow in the leading process mediates axophilic migration. J Cell Biochem 33 11361 PMID: 23843509

Rodríguez-Vita et al (2008) Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation. PLoS One 3 e3959 PMID: 19088845

Sharma et al (2018) Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector. Curr Protoc Mol Biol 122 e58 PMID: 29851250

Schaffer et al (2018) PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep 25 2470 PMID: 30485813

Fazakas et al (2018) Rho-Kinase Inhibition Ameliorates D.nib-Induced Endothelial Dysfunction and Pulmonary Hypertension. Front Physiol 9 537 PMID: 29867576

Ronaldson-Bouchard et al (2018) Advanced maturation of human cardiac tissue grown from pluripotent stem cells. Nature 556 239 PMID: 29618819

Edwards et al (2017) The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ. Sci Signal 10 PMID: 29208682

Hubaud et al (2017) Excitable Dynamics and Yap-Dependent Mechanical Cues Drive the Segmentation Clock. Cell 171 668 PMID: 28942924

Mou et al (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Cell: Stem Cell 19 217 PMID: 27320041

Foulke-Abel et al (2016) Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Nat Commun 150 638 PMID: 26677983

Guye et al (2016) Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Sci Rep 7 10243 PMID: 26732624

Balcioglu et al (2016) Tumor-induced remote ECM network orientation steers angiogenesis. Development 6 22580 PMID: 26931404

Tidball et al (2016) Genomic Instability Associated with p53 Knockdown in the Generation of Huntington's Disease Human Induced Pluripotent Stem Cells. PLoS One 11 e0150372 PMID: 26982737

Neely et al (2012) DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction. Proc Natl Acad Sci U S A 3 482 PMID: 22860217

Guo et al (2012) Slug and Sox9 cooperatively determine the mammary stem cell state. J Cell Sci 148 1015 PMID: 22385965

Walker et al (2012) Intra-axonal translation of RhoA promotes axon growth inhibition by CSPG. J Cell Sci 32 14442 PMID: 23055514

Lee et al (2011) The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder. Dev Biol 15 143 PMID: 21860592

Vallenius et al (2011) An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. Integr Biol (Camb) 124 384 PMID: 21242312

Ruiz and Chen (2008) Emergence of patterned stem cell differentiation within multicellular structures. J Cell Sci 26 2921 PMID: 18703661

Inan and Büyükafşar (2008) Antiepileptic effects of two Rho-kinase inhibitors, Y-27632 and fasudil, in mice. Am J Pathol 155 44 PMID: 18536751

Wilson et al (2016) Cryopreservation of Brain Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Is Enhanced by Rho-Associated Coiled Coil-Containing Kinase Inhibition. Tissue Eng Part C Methods 22 1085 PMID: 27846787

Pei et al (2016) Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluox.. Sci Rep 6 33822 PMID: 27703173

Mulay et al (2016) An in vitro model of murine middle ear epithelium. Dis Model Mech 9 1405 PMID: 27660200

Long et al (2016) Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A. Stem Cells Transl Med 5 1644 PMID: 27484861

Stebbins et al (2016) Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods 101 93 PMID: 26518252

Zeltner et al (2016) Capturing the biology of disease severity in a PSC-based model of familial dysautonomia. Nat Med 22 1421 PMID: 27841875

Kellner et al (2016) Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics. Hippocampus 6 816 PMID: 26748478

Chal et al (2016) Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat.Protoc. 11 1833 PMID: 27583644

Gomez et al (2010) Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction. ACS Chem Neurosci 110 44 PMID: 20336666

Cao et al (2010) Cortical actin dynamics facilitate early-stage centrosome separation. J Pharmacol Exp Ther 20 770 PMID: 20409712

Kim et al (2010) PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. FEBS Lett 107 17206 PMID: 20858732

Wong et al (2018) α-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein. Eneuro 5 PMID: 29497702

Ho et al (2018) Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 59 1969 PMID: 29677358

Sima et al (2018) Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13 54 PMID: 29631617

Medelnik et al (2018) Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells. Stem Cell Reports 10 1751 PMID: 29779899

Levardon et al (2018) Expansion of Airway Basal Cells and Generation of Polarized Epithelium. Bio Protoc 8 PMID: 30009215

McHugh et al (2018) Preclinical comparison of proteasome and ubiquitin E1 enzyme inhibitors in cutaneous squamous cell carcinoma: the identification of mechanisms of differential sensitivity. Oncotarget 9 20265 PMID: 29755650

Hepburn et al (2018) Targeting the spliceosome for cutaneous squamous cell carcinoma therapy: a role for c-MYC and wild-type p53 in determining the degree of tumour selectivity. Oncotarget 9 23029 PMID: 29796170

Ng-Blichfeldt et al (2018) Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation. EBioMedicine 36 461 PMID: 30236449

McKown et al (2018) Impaired adhesion of induced pluripotent stem cell-derived cardiac progenitor cells (iPSC-CPCs) to isolated extracellular matrix from failing hearts. Heliyon 4 e00870 PMID: 30364772

Pirie et al (2018) Mouse genome-wide association studies and systems genetics uncover the genetic architecture associated with hepatic pharmacokinetic and pharmacodynamic properties of a constrained ethyl antisense oligonucleotide targeting Malat1. PLoS Genet 14 e1007732 PMID: 30372444

Rath et al (2018) Rho Kinase Inhibition by AT13148 Blocks Pancreatic Ductal Adenocarcinoma Invasion and Tumor Growth. Cancer Res 78 3321 PMID: 29669760

Ford et al (2018) A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 47 509 PMID: 30458140

Sheng et al (2018) A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nat Commun 9 4047 PMID: 30279449

Ackermann et al (2018) Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections. Nat Commun 9 5088 PMID: 30504915

Pitha et al (2018) Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma. Transl Vis Sci Technol 7 6 PMID: 30479877

Vu et al (2018) Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease. Orphanet J Rare Dis 13 152 PMID: 30220252

Tidball et al (2018) Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts. Bio Protoc 8 PMID: 30320153

Borgs et al (2016) DArgic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects. Scientific Reports 6 33377 PMID: 27640816

Jardé et al (2016) Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids Nature Communications 7 13207 PMID: 27782124

Palpant et al (2016) Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using humanpluripotent stem cells. Nature Protocols 12 15 PMID: 27906170

Pattabiraman et al (2015) RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork. Autophagy 185 496 PMID: 25499974

Lachmann et al (2015) Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies. Nat Commun 4 282 PMID: 25680479

Abraham et al (2015) A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis. Stem Cell Reports 6 7286 PMID: 26129894

Arbab et al (2015) Cloning-free CRISPR. Cell 5 908 PMID: 26527385

Ruprecht et al (2015) Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Int J Mol Sci 160 673 PMID: 25679761

Vergara et al (2015) Transgenic plants as low-cost platform for chemotherapeutic drugs screening. J Biol Chem 16 2174 PMID: 25608652

Lee et al (2015) Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling. Toxicol Sci 290 14740 PMID: 25911094

Tyson et al (2015) Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells. Stem Cells 142 1267 PMID: 25804737

Balcioglu et al (2015) The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions. Nat Biotechnol 128 1316 PMID: 25663698

Morizane et al (2015) Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Nat Commun 33 1193 PMID: 26458176

Chaudhuri et al (2015) Substrate stress relaxation regulates cell spreading. Brain 6 6364 PMID: 25695512

Iovino et al (2015) Early maturation and distinct tau pathology in induced pluripotent stem cell-derived neurons from patients with MAPT mutations. Development 138 3345 PMID: 26220942

Jönsson et al (2015) Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor. Int J Nanomedicine 142 3166 PMID: 26395143

Tai et al (2015) SimV. enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation. Nat Commun 10 5881 PMID: 26451103

Sakaguchi et al (2015) Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue. Mol Vis 6 8896 PMID: 26573335

Su et al (2015) Phenotypes of trypsin- and Col. -prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632. PLoS Comput Biol 21 633 PMID: 26097378

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Magro-Lopez et al (2018) Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids. Stem Cell Res.Ther. 9 186 PMID: 29996941

Sommer et al (2018) Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease. Cell Stem Cell. 23 123 PMID: 29979986

Bantounas et al (2018) Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors. Stem Cell Reports 10 766 PMID: 29429961

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Hien et al (2016) Elevated Glucose Levels Promote Contractile and Cytoskeletal Gene Expression in Vascular Smooth Muscle via Rho/Protein Kinase C and Actin Polymerization. J Biol Chem 291 3552 PMID: 26683376

Koopmans et al (2016) Regulation of actin dynamics by WNT-5A: implications for human airway smooth muscle contraction. Sci Rep 6 30676 PMID: 27468699

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Reviews for Y-27632 dihydrochloride

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Great for single iPS cell survival.
By Anonymous on 09/13/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC

Used during single cell passage for CRISPR clone isolation, works well and no difference found between multiple batches. Cells show characteristic 'spiky' morphology upon addition to the medium.

I use at 1:1000 and store at -20C. I remove the compound from medium 24 hours after plating.

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Human PDAC orgnaoids.
By Anonymous on 05/03/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: tissue

To make Human PDAC orgnaoids


For iPSC culture.
By Pinyuan Tian on 08/07/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC

For iPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS.

Make 100µl aliquots and keep them at −20 °C for up to 6 months.

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ROCK Inhibitor to improve cartilage integrity.
By Anonymous on 03/26/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: chondrocyte

I dissolved in DMSO

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I prepare 20mg of the product in 5.88mL H2O and I use 1uL/mL of the stock in my cell culture after the cell splitting.
By Anonymous on 02/01/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: human foreskin fibroblasts-derived-IPS

I used it in two human IPS cell lines, 5uM concentration the first 24 h after the splitting. The use of this reagent improves substantially the cell survival.

Keep the stock frozen at -20°C. I use it freshly added to the medium when splitting the cells.

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ROCK Inhibitor to improve cell survival.
By Hudson DE OLIVEIRA BEZERRA on 01/05/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: H1 (W01)

My team uses this product to improve human embryonic stem cells survival in single cell passage methods in siRNA and CRISPR experiments. In general, we use the concentration of 10 uM. The products work very well, better than others available in the market.

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