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This is intended as a guide only; for full experimental details please read the reference provided.
Li et al. describe a protocol that uses a cocktail of small molecules to differentiate fibroblasts directly into neurons.
Mouse fibroblasts were cultured for 4 - 5 days. Fibroblast culture medium was then exchanged for induction medium, which resulted in cells with neuron-like morphology. After 20 days these cells were co-cultured with astrocytes in maturation medium, resulting in functional neurons.
|Induction Medium||Maturation Medium||Survival enhancer|
|ISX 9 (Cat.No. 4439)||20 μM||Forskolin (Cat.No. 1099)||10 μM||Y-27632 (Cat.No. 1254)||2 μM|
|Forskolin (Cat.No. 1099)||100 μM||bFGF||50 ng/ml|
|CHIR 99021 (Cat.No. 4423)||20 μM||BDNF||20 ng/ml|
|I-BET 151 (Cat.No. 4650)||0.5 - 2.0 μM||GDNF||20 ng/ml|