Converting Fibroblasts into Cardiomyocytes (9C Cocktail)

This is intended as a guide only; for full experimental details please read the reference provided.

Cao et al. converted human fibroblasts into functional cardiomyocytes using a 9C cocktail. The 9C-treated fibroblasts were transplanted into an infarcted mouse heart and converted to cardiomyocyte-like cells.

Human foreskin fibroblasts (HFF) were treated with 9C for 6 days and CIM for 5 days then transplanted into mice with infarcted hearts. When transplanted, 9C-treated fibroblasts were efficiently converted into cardiomyocyte-like cells.

https://resources.tocris.com/images/protocols/stemcells-image-4.jpg

Cocktails

9C Medium Cardiac Induction Medium
CHIR 99021 (Cat.No. 4423) 10 μM CHIR 99021 (Cat.No. 4423) 12 μM
A 83-01 (Cat.No. 2939) 1 μM BMP-4 25 ng/ml
SC1 (Cat.No. 4433) 1 μM Activin A 10 ng/ml
OAC-2 (Cat.No. 6066) 5 μM VEGF 10 ng/ml
Y-27632 (Cat.No. 1254) 10 μM    
BIX 01294 (Cat.No. 3364) 1 μM    
AS 8351 (Cat.No. 6044) 1 μM    
SU 16f (Cat.No. 3304) 5 μM    
JNJ 10198409 0.1 μM    
https://resources.tocris.com/images/protocols/stemcells-image-8.jpg

Reference

Cao et al. (2016) Conversion of human fibroblasts into functional cardiomyocytes by small molecules. Science 352 6290. PMID: 27127239

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