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This is intended as a guide only; for full experimental details please read the reference provided.
Choi et al. developed a method using small molecules to drive differentiation of hiPSCs into functional myoblasts.
hiPSCs were plated in MEF (mouse embryonic fibroblast)-conditioned N2 medium for the initial stage of myogenic specification. From the next day (day 0) MEF-conditioned N2 medium was exchanged for N2 medium. By day 30 multinucleated spontaneously contractile myotubes were formed. When transplanted into the damaged tibialis anterior muscle of mice, these differentiated cells formed extensive myofibers, without tumor formation.
|MEF-conditioned N2 Medium||N2 Medium Day 0-4||N2 Medium Day 4-12|
|Y-27632 (Cat.No. 1254)||10 μM||CHIR 99021 (Cat.No. 4423)||3 μM||DAPT (Cat.No. 2634)||10 μM|