Go 6983

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Cat.No. 2285 - Go 6983 | C26H26N4O3 | CAS No. 133053-19-7
Description: Broad spectrum PKC inhibitor
Alternative Names: Goe 6983
Chemical Name: 3-[1-[3-(Dimethylamino)propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione
Purity: ≥98% (HPLC)
Datasheet
Citations (23)
Reviews (2)
Literature

Biological Activity

Broad spectrum protein kinase C (PKC) inhibitor (IC50 values are 7, 7, 6, 10, 60 and 20000 nM for PKCα, PKCβ, PKCγ, PKCδ, PKCζ and PKCμ respectively). Displays cardioprotective properties; reduces polymorphonuclear leukocyte adherence and infiltration following myocardial ischemia/reperfusion injury. Optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).

Technical Data

M. Wt 442.51
Formula C26H26N4O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 133053-19-7
PubChem ID 3499
InChI Key LLJJDLHGZUOMQP-UHFFFAOYSA-N
Smiles O=C(N1)C(C2=CNC3=C2C=CC=C3)=C(C4=CN(CCCN(C)C)C5=C4C=C(OC)C=C5)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.12 50

Preparing Stock Solutions

The following data is based on the product molecular weight 442.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.52 mL 22.6 mL 45.2 mL
2.5 mM 0.9 mL 4.52 mL 9.04 mL
5 mM 0.45 mL 2.26 mL 4.52 mL
25 mM 0.09 mL 0.45 mL 0.9 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Gschwendt et al (1996) Inhibition of protein kinase C μ by various inhibitors. Differentiation from protein kinase c isozymes. FEBS Lett. 392 77 PMID: 8772178

Peterman et al (2004) Go 6983 exerts cardioprotective effects in myocardial ischemic/reperfusion. J.Cardiovasc.Pharmacol. 43 645 PMID: 15071351

Young et al (2005) Go 6983: a fast acting protein kinase C inhibitor that attenuates myocardial ischemia/reperfusion injury. Cardiovasc.Drug Rev. 23 255 PMID: 16252018

Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282 PMID: 24172903


If you know of a relevant reference for Go 6983, please let us know.

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View all Protein Kinase C Inhibitors

Keywords: Go 6983, Go 6983 supplier, Broad, spectrum, PKC, inhibitors, inhibits, Protein, Kinases, C, Go6983, Goe6983, stem, cells, naive, Goe, 6983, Kinase, Stem, Cell, Proliferation, 2285, Tocris Bioscience

23 Citations for Go 6983

Citations are publications that use Tocris products. Selected citations for Go 6983 include:

Messmer et al (2019) Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution. Cell Rep 26 815 PMID: 30673604

Pakpour et al (2013) Protein kinase C-dependent signaling controls the midgut epithelial barrier to malaria parasite infection in anopheline mosquitoes. PLoS One 8 e76535 PMID: 24146884

Vliet et al (2013) Human T cell activation results in extracellular signal-regulated kinase (ERK)-calcineurin-dependent exposure of Tn antigen on the cell surface and binding of the macrophage galactose-type lectin (MGL). J Biol Chem 288 27519 PMID: 23918927

Rajendran et al (2013) Inhibition of protein kinase C signaling maintains rat embryonic stem cell pluripotency. J Biol Chem 288 24351 PMID: 23846691

Schaffer et al (2018) PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep 25 2470 PMID: 30485813

Toda and Huganir (2015) Regulation of AMPA receptor phosphorylation by the neuropeptide PACAP38. Nat Commun 112 6712 PMID: 25964356

Liu et al (2018) Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish. Transl Psychiatry 8 232 PMID: 30352990

Fang et al (2018) A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells. Nat Commun 9 4384 PMID: 30349051

Augustin et al (2017) Reduced ins. signaling maintains electrical transmission in a neural circuit in aging flies. PLoS Biol 15 e2001655 PMID: 28902870

Zhang et al (2017) Protein kinase D at the Golgi controls NLRP3 inflammasome activation. J Exp Med 214 2671 PMID: 28716882

Yi et al (2014) Systematic analysis of bacterial effector-postsynaptic density 95/disc large/zonula occludens-1 (PDZ) domain interactions demonstrates Shigella OspE protein promotes protein kinase C activation via PDLIM proteins. Neuron 289 30101 PMID: 25124035

Kuan et al (2014) Ets and GATA Transcription Factors Play a Critical Role in PMA-Mediated Repression of the ckβ Promoter via the Protein Kinase C Signaling Pathway. Biol Chem 9 e113485 PMID: 25490397

Cavaliere et al (2014) NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation. Cell Signal 7 261 PMID: 24391542

Atwood et al (2013) GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas. J Neurosci 494 484 PMID: 23446420

Dutta et al (2011) Self-renewal versus lineage commitment of embryonic stem cells: protein kinase C signaling shifts the balance. Stem Cells 29 618 PMID: 21308862

Devinney et al (2015) Phrenic long-term facilitation requires PKCθ activity within phrenic motor neurons. Proc Natl Acad Sci U S A 35 8107 PMID: 26019328

Shakiba et al (2015) CD24 tracks divergent pluripotent states in mouse and human cells. J Biol Chem 6 7329 PMID: 26076835

Tuvikene et al (2016) AP-1 Transcription Factors Mediate BDNF-Positive Feedback Loop in Cortical Neurons. J Neurosci 36 1290 PMID: 26818516

Carriba (2017) CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6 PMID: 29111976

Baskin and Sayeski (2012) Angiotensin II mediates cell survival through upregulation and activation of the serum and glucocorticoid inducible kinase 1. BMC Complement Altern Med 24 435 PMID: 21963429

Li et al (2016) Progesterone receptor membrane component-1 regulates hepcidin biosynthesis. J Clin Invest 126 389 PMID: 26657863

Scarisbrick et al (2012) Kallikrein 6 is a novel molecular trigger of reactive astrogliosis. Front Cell Neurosci 393 355 PMID: 22505518

Garrett et al (2012) γ-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. PLoS One 74 269 PMID: 22542181


Do you know of a great paper that uses Go 6983 from Tocris? Please let us know.

Reviews for Go 6983

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Go 6983 blocks LPA-mediated COX-2 expression.
By Anonymous on 12/13/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Osteosarcoma

Human osteosarcoma cells were incubated with 20 μM Go 6983 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis. Go 6983 abolished LPA-induced COX-2 expression.

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Naive human pluripotent stem cell culture.
By Anonymous on 05/31/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: pluripotent stem cells

Used for the culture of naive human pluripotent stem cells in t2iL+go media. Go 6983 used at 2µM concentration.

PMID: 28343983

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