SP 600125

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Description: Selective JNK inhibitor
Chemical Name: Anthra[1-9-cd]pyrazol-6(2H)-one
Purity: ≥98% (HPLC)
Citations (108)
Reviews (4)
Literature (1)
Pathways (1)

Biological Activity for SP 600125

SP 600125 is a selective JNK inhibitor. Competitively and reversibly inhibits JNK1, 2 and 3 (IC50 = 40 - 90 nM) with negligible activity at ERK2, p38β and a range of enzymes. Active in vivo. Exhibits reduced selectivity over other protein kinases under certain conditions. SP 600125 protects renal tubular epithelial cells from ischemia/reperfusion-induced apoptosis. Promotes chondrogenesis of bone mesenchymal stem cells by inhibition of TNF-α-induced inflammation. Adipogenesis from MSCs is also promoted by SP 600125. Essential component of medium for maintaining stem cells in naive pluripotent state.

SP 600125 synthesized to Ancillary Material Grade also available.

Technical Data for SP 600125

M. Wt 220.23
Formula C14H8N2O
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 129-56-6
PubChem ID 8515
Smiles O=C1C2=C3C(NN=C3C3=CC=CC=C13)=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SP 600125

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 22.02 100

Preparing Stock Solutions for SP 600125

The following data is based on the product molecular weight 220.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.54 mL 22.7 mL 45.41 mL
5 mM 0.91 mL 4.54 mL 9.08 mL
10 mM 0.45 mL 2.27 mL 4.54 mL
50 mM 0.09 mL 0.45 mL 0.91 mL

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Product Datasheets for SP 600125

Certificate of Analysis / Product Datasheet
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References for SP 600125

References are publications that support the biological activity of the product.

Bennett et al (2001) SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc.Natl.Acad.Sci.U.S.A. 98 13681 PMID: 11717429

Schnabl et al (2001) TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells. Hepatology 34 953 PMID: 11679966

Wang et al (2007) SP600125, a selective JNK inhibitor, protects ischemic renal injury via suppressing the extrinsic pathways of apoptosis. Life Sci. 80 2067 PMID: 17459422

Zhao et al (2013) Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells. BMB Rep. 46 422 PMID: 23977991

Ding et al (2021) SP600125 restored TNF-α-induced impaired chondrogenesis in bone mesenchymal stem cells and its antiosteoarthritis effect in mice. Stem Cells Dev. 30 1028 PMID: 34486378

Tominaga et al (2005) Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase. Biochem.Biophys.Res.Commun. 326 499 PMID: 15582605

If you know of a relevant reference for SP 600125, please let us know.

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Keywords: SP 600125, SP 600125 supplier, selective, JNK, inhibitors, inhibits, MAPK, Signaling, Signalling, c-Jun, N-Terminal, Kinase, SAPKs, Stress-Activated, Protein, Mitogen-Activated, SP600125, JNK/c-jun, Mesenchymal, Stem, Cells, Cell, Proliferation, 1496, Tocris Bioscience

108 Citations for SP 600125

Citations are publications that use Tocris products. Selected citations for SP 600125 include:

Yu et al (2009) c-Jun protects hypoxia-inducible factor-1alpha from degradation via its oxygen-dependent degradation domain in a nontranscriptional manner. Cancer Res 69 7704 PMID: 19738058

Yun et al (2009) AMP kinase signaling determines whether c-Jun N-terminal kinase promotes survival or apoptosis during glucose deprivation. Carcinogenesis 30 529 PMID: 19037093

Steubesand et al (2009) The expression of the beta-defensins hBD-2 and hBD-3 is differentially regulated by NF-kappaB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis. BMC Immunol 10 36 PMID: 19523197

Yeligar et al (2009) Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1. J Immunol 183 5964 PMID: 19828633

Suk et al (2013) A Taiwanese Propolis Derivative Induces Apoptosis through Inducing Endoplasmic Reticular Stress and Activating Transcription Factor-3 in Human Hepatoma Cells. Evid Based Complement Alternat Med 2013 658370 PMID: 24222778

Berdichevsky et al (2013) PI3K-Akt signaling activates mTOR-mediated epileptogenesis in organotypic hippocampal culture model of post-traumatic epilepsy. J Neurosci 33 9056 PMID: 23699517

Wong et al (2010) Bone morphogenic protein-4 impairs endothelial function through oxidative stress-dependent cyclooxygenase-2 upregulation: implications on hypertension. Circ Res 107 984 PMID: 20724703

Sutcliffe et al (2009) Transcriptional regulation of monocyte chemotactic protein-1 release by endothelin-1 in human airway smooth muscle cells involves NF-kappaB and AP-1. Br J Pharmacol 157 436 PMID: 19371341

Hendricks and Jesuthasan (2009) PHR regulates growth cone pausing at intermediate targets through microtubule disassembly. J Neurosci 29 6593 PMID: 19458229

Balboa et al (2018) Ins. mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes. Elife 7 PMID: 30412052

Kang et al (2018) Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys. Cell Rep 25 2563 PMID: 30485820

Maronde (2018) Trehalose Activates CRE-Dependent Transcriptional Signaling in HT22 Mouse Hippocampal Neuronal Cells: A Central Role for PKA Without cAMP Elevation. Front Mol Neurosci 11 386 PMID: 30405349

Lin et al (2018) Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells. Cancer Manag Res 10 4237 PMID: 30323679

Yang et al (2017) Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell 169 243 PMID: 28388409

Harada et al (2017) Focal Adhesion Kinase Promotes the Progression of Aortic Aneurysm by Modulating Macrophage Behavior. Arterioscler Thromb Vasc Biol 37 156 PMID: 27856458

Edén et al (2015) Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated Ins secretion from human pancreatic islets. Diabetologia 58 2563 PMID: 26271343

Zhang et al (2015) Activation of transcription factor AP-1 in response to thermal injury in rat small intestine and IEC-6 cells. BMC Gastroenterol 15 83 PMID: 26162907

Huang et al (2013) The role of bioactive nanofibers in enamel regeneration mediated through integrin signals acting upon C/EBPα and c-Jun. Biomaterials 34 3303 PMID: 23398885

Kook et al (2013) Fibroblast growth factor-4 enhances proliferation of mouse embryonic stem cells via activation of c-Jun signaling. PLoS One 8 e71641 PMID: 23967228

Morse et al (2013) Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family. BMC Pharmacol Toxicol 14 17 PMID: 23497702

Meotti et al (2012) Inflammatory muscle pain is dependent on the activation of kinin B1 and B2 receptors and intracellular kinase pathways. Br J Pharmacol 166 1127 PMID: 22220695

Schepetkin et al (2012) Identification and characterization of a novel class of c-Jun N-terminal kinase inhibitors. Mol Pharmacol 81 832 PMID: 22434859

Lin et al (2012) Desipramine protects neuronal cell death and induces heme oxygenase-1 expression in Mes23.5 DArgic neurons. PLoS One 7 e50138 PMID: 23209658

Khuituan et al (2012) Fibroblast growth factor-23 abolishes 1,25-dihydroxyvitamin D3-enhanced duodenal calcium transport in male mice. Am J Physiol Endocrinol Metab 302 E903 PMID: 22275752

Lee et al (2010) Prevention of premature senescence requires JNK regulation of Bcl-2 and reactive oxygen species. Oncogene 29 561 PMID: 19855432

Ishida et al (2010) Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish. Dev Biol 340 468 PMID: 20144602

Zhou et al (2010) miR-155 and its star-form partner miR-155* cooperatively regulate type I IF. production by human plasmacytoid dendritic cells. Blood 116 5885 PMID: 20852130

Hamnett et al (2019) Vasoactive intestinal peptide controls the suprachiasmatic circadian clock network via ERK1/2 and DUSP4 signalling. Nat Commun 10 542 PMID: 30710088

Heckler et al (2014) ERK/MAPK regulates ERRγ expression, transcriptional activity and receptor-mediated tamox. resistance in ER+ breast cancer. FEBS J 281 2431 PMID: 24684682

Wei et al (2014) Central SDF-1/CXCL12 expression and its cardiovascular and sympathetic effects: the role of angiotensin II, TNF-α, and MAP kinase signaling. Am J Physiol Heart Circ Physiol 307 H1643 PMID: 25260613

Ulzii et al (2019) Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes. Int J Mol Sci 20 PMID: 31284553

Zhen et al (2019) Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5. Biomol Ther (Seoul) 27 395 PMID: 30419635

Wu (2017) TRIB3 downregulation enhances doxorubicin-induced cytotoxicity in gastric cancer cells. Arch Biochem Biophys 622 26 PMID: 28442401

Han (2017) Cannabinoid receptor 1 contributes to sprouted innervation in endometrial ectopic growth through mitogen-activated protein kinase activation. Brain Res 1663 132 PMID: 28322749

Zhou (2017) TNFα induces tolerant production of CXC chemokines in colorectal cancer HCT116 cells via A20 inhibition of ERK signaling. Int Immunopharmacol 54 296 PMID: 29175508

Yuri et al (2015) Maintenance of Mouse Nephron Progenitor Cells in Aggregates with Gamma-Secretase Inhibitor. PLoS One 10 e0129242 PMID: 26075891

Akhmetshina et al (2012) Activation of canonical Wnt signalling is required for TGF-β-mediated fibrosis. Stem Cells Int 3 735 PMID: 22415826

Oberbach et al (2012) Free fatty acid palmitate impairs the vitality and function of cultured human bladder smooth muscle cells. Am J Physiol Cell Physiol 7 e41026 PMID: 22808290

Chen et al (2012) Cyclic AMP increases COX-2 expression via mitogen-activated kinase in human myometrial cells. PLoS One 16 1447 PMID: 21854542

Ye et al (2011) Dermatophagoides pteronyssinus 2 regulates nerve growth factor release to induce airway inflammation via a reactive oxygen species-dependent pathway. J Inflamm (Lond) 300 L216 PMID: 21097523

Kim et al (2011) Thrombin-induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells. Nat Commun 15 211 PMID: 21994479

Hsu et al (2011) JNK suppression is essential for 17β-OE inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells. PLoS One 18 61 PMID: 21859479

Hecker et al (2011) Reversible differentiation of myofibroblasts by MyoD. J Biol Chem 317 1914 PMID: 21440539

Jezierska-Drutel et al (2019) The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC Cancer 19 812 PMID: 31419957

Pervolaraki et al (2017) Type I and Type III IF.s Display Different Dependency on Mitogen-Activated Protein Kinases to Mount an Antiviral State in the Human Gut. Front Immunol 8 459 PMID: 28484457

Sidhar and Giri (2017) Induction of Bex genes by curcumin is associated with apoptosis and activation of p53 in N2a neuroblastoma cells. Sci Rep 7 41420 PMID: 28145533

Martínez-Sánchez et al (2017) Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. Cell Metab 26 212 PMID: 28683288

Zhang et al (2014) Adiponectin ameliorates experimental periodontitis in diet-induced obesity mice. PLoS One 9 e97824 PMID: 24836538

Yu et al (2014) Differential effects of the Toll-like receptor 2 agonists, PGN and Pam3CSK4 on anti-IgE induced human mast cell activation. PLoS One 9 e112989 PMID: 25398056

Kim et al (2014) AMP-activated protein kinase determines apoptotic sensitivity of cancer cells to ginsenoside-Rh2. J Ginseng Res 38 16 PMID: 24558305

Kim et al (2014) Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose. J Phys Ther Sci 26 1757 PMID: 25435694

Gonsalves et al (2014) Angiogenic growth factors augment K-Cl cotransporter expression in erythroid cells via hypoxia-inducible factor-1α. Am J Hematol 89 273 PMID: 24227191

Bae et al (2014) Activation of pattern recognition receptors in brown adipocytes induces inflammation and suppresses uncoupling protein 1 expression and mitochondrial respiration. Am J Physiol Cell Physiol 306 C918 PMID: 24627558

Basu et al (2014) Suppression of MAPK/JNK-MTORC1 signaling leads to premature loss of organelles and nuclei by autophagy during terminal differentiation of lens fiber cells. Autophagy 10 1193 PMID: 24813396

Huang et al (2014) Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells. BMC Complement Altern Med 14 358 PMID: 25256260

Enomoto et al (2013) Transcriptional regulation of an Ins-sensitizing adipokine adipolin/CTRP12 in adipocytes by Krüppel-like factor 15. Am J Physiol Lung Cell Mol Physiol 8 e83183 PMID: 24358263

Stradner et al (2013) Sphingosine 1-phosphate counteracts the effects of interleukin-1β in human chondrocytes. Genes Cancer 65 2113 PMID: 23666803

Nalvarte et al (2016) Estrogen receptor β controls MMP-19 expression in mouse ovaries during ovulation. Reproduction 151 253 PMID: 26700939

Chang et al (2016) Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells. PLoS One 11 e0165715 PMID: 27802323

Abedini et al (2016) WNT5a is required for normal ovarian follicle development and antagonizes g.tropin responsiveness in granulosa cells by suppressing canonical WNT signaling. FASEB J 30 1534 PMID: 26667040

Kang et al (2011) MUC5AC expression through bidirectional communication of Notch and epidermal growth factor receptor pathways. J Immunol 187 222 PMID: 21622856

García-Escudero et al (2008) Glioma regression in vitro and in vivo by a suicide combined treatment. Exp Cell Res 6 407 PMID: 18337448

Wan et al (2018) Suppressive effects of berberine on atherosclerosis via downregulating visfatin expression and attenuating visfatin-induced endothelial dysfunction. Int J Mol Med 41 1939 PMID: 29393413

Kim et al (2018) Enhanced Glycolysis Supports Cell Survival in EGFR-Mutant Lung Adenocarcinoma by Inhibiting Autophagy-Mediated EGFR Degradation. Cancer Res 78 4482 PMID: 29945964

Rahman et al (2016) 18α-glycyrrhetinic acid lethality for neuroblastoma cells via de-regulating the Beclin-1/Bcl-2 complex and inducing apoptosis. Biochemical Pharmacology 117 97 PMID: 27520483

Sommerfeld et al (2015) Free fatty acids shift Ins-induced hepatocyte proliferation towards CD95-dependent apoptosis. J Biomed Sci 290 4398 PMID: 25548285

Karmakar et al (2015) Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation and is dependent on K+ efflux. Nat Commun 194 1763 PMID: 25609842

Liu et al (2015) Hyperphosphate-Induced Myocardial Hypertrophy through the GATA-4/NFAT-3 Signaling Pathway Is Attenuated by ERK Inhibitor Treatment. Int J Mol Sci 5 79 PMID: 25999956

Yoshimura et al (2015) Inhibitory effect of STAT on inflammation-related pathways in human abdominal aortic aneurysm tissue. Biomed Res Int 16 11213 PMID: 25993292

Chen et al (2015) Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway. Int J Clin Exp Pathol 2015 414250 PMID: 26649302

Bulut et al (2015) Effects of jnk inhibitor on inflammation and fibrosis in the ovary tissue of a rat model of polycystic ovary syndrome. Exp Hematol Oncol 8 8774 PMID: 26464620

Koerber et al (2015) Analysis of the anti-proliferative and the pro-apoptotic efficacy of Syk inhibition in multiple myeloma. Nat Commun 4 21 PMID: 26251761

Yang et al (2015) Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. PLoS One 2015 270428 PMID: 26089912

Smith et al (2015) The Effects of the Endocannabinoids Anandamide and 2-Arachidonoylglycerol on Human Osteoblast Proliferation and Differentiation. J Biol Chem 10 e0136546 PMID: 26414859

Martin et al (2014) miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling. Korean J Physiol Pharmacol 5 353 PMID: 25352952

Alpár et al (2014) Endocannabinoids modulate cortical development by configuring Slit2/Robo1 signalling. Steroids 5 4421 PMID: 25030704

El-Sayed et al (2014) P2Y2 nucleotide receptor activation enhances the aggregation and self-organization of dispersed salivary epithelial cells. J Cell Mol Med 307 C83 PMID: 24760984

Xiong et al (2013) Arginase-II induces vascular smooth muscle cell senescence and apoptosis through p66Shc and p53 independently of its l-arginine ureahydrolase activity: implications for atherosclerotic plaque vulnerability. J Am Heart Assoc 2 e000096 PMID: 23832324

Tsai et al (2011) Carnosic acid induces the NAD(P)H: quinone oxidoreductase 1 expression in rat clone 9 cells through the p38/nuclear factor erythroid-2 related factor 2 pathway. J Nutr 141 2119 PMID: 22031657

Morse et al (2011) Ligand-directed functional selectivity at the mu opioid receptor revealed by label-free integrative pharmacology on-target. PLoS One 6 e25643 PMID: 22003401

Zhou and Too (2011) Mitochondrial localized STAT3 is involved in NGF induced neurite outgrowth. Arthritis Rheum 6 e21680 PMID: 21738764

Zuco et al (2004) Induction of apoptosis and stress response in ovarian carcinoma cell lines treated with ST1926, an atypical retinoid. Cell Death Differ 11 280 PMID: 14657960

Holzberg et al (2003) Disruption of the c-JUN-JNK complex by a cell-permeable peptide containing the c-JUN delta domain induces apoptosis and affects a distinct set of interleukin-1-induced inflammatory genes. J Biol Chem 278 40213 PMID: 12832416

Stähle et al (2003) Mechanisms in LPA-induced tumor cell migration: critical role of phosphorylated ERK. J Cell Sci 116 3835 PMID: 12902401

Campàs et al (2003) Acadesine activates AMPK and induces apoptosis in B-cell chronic lymphocytic leukemia cells but not in T lymphocytes. Cell Death Differ 101 3674 PMID: 12522004

Ma et al (2005) Oxygen-sensitive {δ}-opioid receptor-regulated survival and death signals: novel insights into neuronal preconditioning and protection. PLoS One 280 16208 PMID: 15687501

Yun et al (2005) Glucose deprivation increases mRNA stability of vascular endothelial growth factor through activation of AMP-activated protein kinase in DU145 prostate carcinoma. Blood 280 9963 PMID: 15640157

Kim et al (2006) Transforming growth factor-beta1 regulates macrophage migration via RhoA. Blood 108 1821 PMID: 16705092

Kauppinen et al (2006) Direct phosphorylation and regulation of poly(ADP-ribose) polymerase-1 by extracellular signal-regulated kinases 1/2. Proc Natl Acad Sci U S A 103 7136 PMID: 16627622

Wang et al (2006) PacT. (Taxol) upregulates expression of functional interleukin-6 in human ovarian cancer cells through multiple signaling pathways. Oncogene 25 4857 PMID: 16547493

Sunters et al (2006) PacT.-induced nuclear translocation of FOXO3a in breast cancer cells is mediated by c-Jun NH2-terminal kinase and Akt. Cancer Res 66 212 PMID: 16397234

Guillemot and Citi (2006) Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. Mol Biol Cell 17 3569 PMID: 16723500

Olsson and Sundler (2006) Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages. J Immunol 3 8 PMID: 16674821

Kuntzen et al (2005) Inhibition of c-Jun-N-terminal-kinase sensitizes tumor cells to CD95-induced apoptosis and induces G2/M cell cycle arrest. Cancer Res 65 6780 PMID: 16061660

Hoffmann et al (2005) MEK1-dependent delayed expression of Fos-related antigen-1 counteracts c-Fos and p65 NF-kappaB-mediated interleukin-8 transcription in response to cytokines or growth factors. J Biol Chem 280 9706 PMID: 15615716

Lee et al (2005) Estrogen receptor alpha pathway is involved in the regulation of Calbindin-D9k in the uterus of immature rats. Toxicol Sci 84 270 PMID: 15635152

Zhou et al (2007) Delayed and persistent ERK1/2 activation is required for 4-hydroxytamoxifen-induced cell death. Mol Cancer Res 72 765 PMID: 17714751

Shacka et al (2006) Two distinct signaling pathways regulate peroxynitrite-induced apoptosis in PC12 cells. Cardiorenal Med 13 1506 PMID: 16410804

Toyoda et al (2007) Requirement of extracellular signal-regulated kinase/mitogen-activated protein kinase for long-term potentiation in adult mouse anterior cingulate cortex. Mol Pain 3 36 PMID: 18053155

Schlee et al (2007) Induction of human beta-defensin 2 by the probiotic Escherichia coli Nissle 1917 is mediated through flagellin. Infect Immun 75 2399 PMID: 17283097

Su et al (2007) 5-Aminoimidazole-4-carboxamide riboside sensitizes TRAIL- and TNF{alpha}-induced cytotoxicity in colon cancer cells through AMP-activated protein kinase signaling. Mol Cancer Ther 6 1562 PMID: 17513605

Chen et al (2008) Ceramide induces p38 MAPK and JNK activation through a mechanism involving a thioredoxin-interacting protein-mediated pathway. Blood 111 4365 PMID: 18270325

Su et al (2008) 15-deoxy-Delta12,14-prostaglandin J2 up-regulates death receptor 5 gene expression in HCT116 cells: involvement of reactive oxygen species and C/EBP homologous transcription factor gene transcription. Mol Cancer Ther 7 3429 PMID: 18852146

Jung et al (2008) Reactive oxygen species stabilize hypoxia-inducible factor-1 alpha protein and stimulate transcriptional activity via AMP-activated protein kinase in DU145 human prostate cancer cells. Carcinogenesis 29 713 PMID: 18258605

Wolter et al (2008) c-Jun controls histone modifications, NF-kappaB recruitment, and RNA polymerase II function to activate the ccl2 gene. Mol Cell Biol 28 4407 PMID: 18443042

Moynihan et al (2008) MAP kinases mediate interleukin-13 effects on calcium signaling in human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 295 L171 PMID: 18441092

Hoffmann et al (2008) Transcriptional regulation of EGR-1 by the interleukin-1-JNK-MKK7-c-Jun pathway. J Biol Chem 283 12120 PMID: 18281687

Wei et al (2008) Mitogen-activated protein kinases mediate upregulation of hypothalamic angiotensin II type 1 receptors in heart failure rats. Hypertension 52 679 PMID: 18768402

Do you know of a great paper that uses SP 600125 from Tocris? Please let us know.

Reviews for SP 600125

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Role of JNK activation in chemotherapy-induced apoptosis.
Assay Type: In Vitro
Species: Human

Human cancer cells were pretreated for 2 hours with SP600125 before addition of chemotherapeutics and were incubated for 72 hours. SP600125 was used at 1, 3 and 10 μM. Real time apoptosis assays were performed. JNK inhibition partially reversed the chemotherapeutics-induced cellular apoptosis.

JNK inhibition in LPA induced pro-inflammatory phenotype in microglia..
By lisha joshi on 06/10/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Primary murine microglia cells

Primary murine microglia were treated with LPA (1µM) in the presence or absence of SP600125 (10µM) in order to investigate the role of JNK pathway in the LPA induced inflammatory phenotype. The inhibitor was well tolerated by the cells. DMSO was used as a vehicle control. Cells were lysed and collected for WB analysis in order to identify changes in the expression of COX2 and Arg1.

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SP 600125 inhibits JNK activation in HOCl-LDL treated cardiomyocytes.
By Chintan Koyani on 10/13/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: HL-1 cardiomyocytes

HL-1 cardiomyocytes were treated with SP 600125 (5 or 10 μM, 30 min) followed by 500 μg/ml HOCl-LDL (oxidant:lipoprotein molar ratio=800:1) for 1 h to follow pJNK expression using Western blot. SP 600125 at 10 μM concentration, completely inhibited activation of JNK.

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JNK inhibition in BV2 microglia.
By Joanna Plastira on 10/10/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: BV2 microglia

The inhibitor was used at a concentration of 10µM in BV2 microglia cell line. SP600125 inhibited the LPA induced activation of transcription factors (western blotting). It was also used in flow cytometry, ELISAs, IF and functional assays. The results were constant and reproducible.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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MAPK Signaling Scientific Review

MAPK Signaling Scientific Review

MAP kinase signaling is integral to the regulation of numerous cellular processes such as proliferation and differentiation, and as a result is an important focus of cancer and immunology research. Updated for 2016, this review discusses the regulation of the MAPK pathway and properties of MAPK cascades. Compounds available from Tocris are listed.

Pathways for SP 600125

MAPK Signaling Pathway

MAPK Signaling Pathway

The mitogen-activated protein kinase pathway evokes an intracellular signaling cascade in response to extracellular stimuli such as heat and stress. It can influence cell division, metabolism and survival.