DAPI

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Cat.No. 5748 - DAPI | C16H15N5.2HCl | CAS No. 28718-90-3

Description: Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided

Alternative Names: DAPI Dye

Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride

Purity: ≥95% (HPLC)

Datasheet

Citations

Reviews (2)

Literature (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for DAPI

DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.

Use of DAPI Stain as Nuclear Counterstain in HeLa Cells for DAPI

DAPI stain as nuclear counterstain on HeLa cells

3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid dyes 520, 570 and 650. Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data for DAPI

Emission Color	Blue
λ_abs	350 nm
λ_em	470 nm
Application	Flow cytometry, ISH

Technical Data for DAPI

M. Wt	350.25
Formula	C₁₆H₁₅N₅.2HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	28718-90-3
PubChem ID	160166
InChI Key	FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles	N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DAPI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	35.02	100
	water	3.5	10 with gentle warming

Preparing Stock Solutions for DAPI

The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.86 mL	14.28 mL	28.55 mL
5 mM	0.57 mL	2.86 mL	5.71 mL
10 mM	0.29 mL	1.43 mL	2.86 mL
50 mM	0.06 mL	0.29 mL	0.57 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

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Product Datasheets for DAPI

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for DAPI

References are publications that support the biological activity of the product.

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301

Chazotte (2011) Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 1 prot5556 PMID: 21205856

Kapuscinski (1995) DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 70 220 PMID: 858020

Sandell et al (2018) DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 10 prot094029 PMID: 30275072

If you know of a relevant reference for DAPI, please let us know.

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Keywords: DAPI, DAPI supplier, fluorescent, DNA, stains, cell, permeable, dpi, dyes, apoptosis, staining, nuclear, counterstain, flow, cytometry, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience

Citations for DAPI

Citations are publications that use Tocris products.

Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? Please let us know.

Reviews for DAPI

Average Rating: 4.5 (Based on 2 Reviews.)

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DAPI staining of PC3 cells to detect apoptosis.

By Anonymous on 12/13/2018

Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.

Excellent counter-stain....

By Purnima Gupta on 01/10/2018

Assay Type: In Vivo

Species: Mouse

Cell Line/Tissue: Brain

Used as counter stain in IHC

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our

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Bioluminescent Substrates
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