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Description: Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95% (HPLC)
Citations (14)
Reviews (2)
Literature (1)

Biological Activity for DAPI

DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.

Scientific Data

Confocal Microscopy DAPI staining View Larger

DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.

Confocal Microscopy DAPI counterstaining alongside MitoBrilliant™ Live 646 View Larger

DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.

Fluorescence Imaging RNAScope™ Assay with DAPI Counterstain View Larger

RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data for DAPI

DAPI Dye Spectra
Emission Color Blue
λabs 350 nm
λem 470 nm
Closest Laser line 405 nm
Application Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for DAPI

M. Wt 350.25
Formula C16H15N5.2HCl
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 28718-90-3
PubChem ID 160166
Smiles N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DAPI

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 35.02 100
water 3.5 10 with gentle warming

Preparing Stock Solutions for DAPI

The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

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Product Datasheets for DAPI

Certificate of Analysis / Product Datasheet
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References for DAPI

References are publications that support the biological activity of the product.

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301

Chazotte (2011) Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 1 prot5556 PMID: 21205856

Kapuscinski (1995) DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 70 220 PMID: 858020

Sandell et al (2018) DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 10 prot094029 PMID: 30275072

If you know of a relevant reference for DAPI, please let us know.

Keywords: DAPI, DAPI supplier, fluorescent, DNA, stains, cell, permeable, dpi, dyes, apoptosis, staining, nuclear, counterstain, flow, cytometry, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience

14 Citations for DAPI

Citations are publications that use Tocris products. Selected citations for DAPI include:

Elizabeth et al (2021) A protocol for rapid pericyte differentiation of human induced pluripotent stem cells. STAR Protoc 2 100261 PMID: 33490977

Daniel F et al (2021) Granzyme A Produced by γ9δ2 T Cells Activates ER Stress Responses and ATP Production, and Protects Against Intracellular Mycobacterial Replication Independent of Enzymatic Activity. Front Immunol 12 712678 PMID: 34413857

Digvijay et al (2021) HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Science 371 PMID: 33335017

Thanaphum et al (2020) NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells. Sci Rep 10 13329 PMID: 32770090

Dean P et al (2020) Gut-Resident Lactobacilli Activate Hepatic Nrf2 and Protect Against Oxidative Liver Injury. Cell Metab 31 956-968.e5 PMID: 32213347

Allen et al (2020) Indirect regulation of HMGB1 release by gasdermin D. Nat Commun 11 4561 PMID: 32917873

Thanaphum et al (2023) Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells. J Dent Sci 18 105-111 PMID: 36643268

Yelyzaveta et al (2023) BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity. Nat Commun 14 1085 PMID: 36841849

Yu et al (2023) Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming. Cell Mol Gastroenterol Hepatol 15 1255-1275 PMID: 36706918

Paul L et al (2023) Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase. Biomolecules 13 PMID: 37189407

Satu et al (2022) Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance. J Exp Med 219 PMID: 35050301

Titikorn et al (2022) Modulation of mitochondrial dynamics rescues cognitive function in rats with 'doxorubicin-induced chemobrain' via mitigation of mitochondrial dysfunction and neuroinflammation. FEBS J 289 6435-6455 PMID: 35514149

Marit H et al (2022) A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39 110663 PMID: 35417692

Nipon et al (2022) Erythropoietin administration exerted neuroprotective effects against cardiac ischemia/reperfusion injury. Curr Res Pharmacol Drug Discov 3 100124 PMID: 36568264

Do you know of a great paper that uses DAPI from Tocris? Please let us know.

Reviews for DAPI

Average Rating: 4.5 (Based on 2 Reviews.)

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DAPI staining of PC3 cells to detect apoptosis.
By Anonymous on 12/13/2018
Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.

review image

Excellent counter-stain....
By Purnima Gupta on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

Used as counter stain in IHC

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals