Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewDAPI
Description: Fluorescent DNA dye; dapi can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95% (HPLC)
Biological Activity for DAPI
DAPI is a fluorescent DNA stain / dye. DAPI binds to AT-rich regions of DNA. DAPI can be used in flow cytometry and to assess apoptosis. DAPI is cell permeable at high concentrations providing effective cell staining. Em/Ex λ = 350/470 nm respectively.
Optical Data for DAPI
| λabs | 350 nm |
|---|---|
| λem | 470 nm |
| Application | Flow cytometry |
Technical Data for DAPI
| M. Wt | 350.25 |
| Formula | C16H15N5.2HCl |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 28718-90-3 |
| PubChem ID | 160166 |
| InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Smiles | N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DAPI
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.02 | 100 | |
| water | 3.5 | 10 with gentle warming |
Preparing Stock Solutions for DAPI
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for DAPI
References for DAPI
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
If you know of a relevant reference for DAPI, please let us know.
View Related Products by Target
Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, dpi, dyes, apoptosis, staining, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience
Citations for DAPI
Citations are publications that use Tocris products.
Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? Please let us know.
Reviews for DAPI
Average Rating: 4.5 (Based on 2 Reviews.)
Have you used DAPI?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
DAPI staining of PC3 cells to detect apoptosis.
By
Anonymous on 12/13/2018
Species: Human
DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Excellent counter-stain....
By
Purnima Gupta on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain
Used as counter stain in IHC
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals