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Cat.No. 5748 - DAPI | C16H15N5.2HCl | CAS No. 28718-90-3
Description: Fluorescent DNA dye; dapi can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95% (HPLC)
Reviews (2)
Literature (1)

Biological Activity for DAPI

DAPI is a fluorescent DNA stain / dye. DAPI binds to AT-rich regions of DNA. DAPI can be used in flow cytometry and to assess apoptosis. DAPI is cell permeable at high concentrations providing effective cell staining. Em/Ex λ = 350/470 nm respectively.

Optical Data for DAPI

λabs 350 nm
λem 470 nm
Application Flow cytometry

Technical Data for DAPI

M. Wt 350.25
Formula C16H15N5.2HCl
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 28718-90-3
PubChem ID 160166
Smiles N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DAPI

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 35.02 100
water 3.5 10 with gentle warming

Preparing Stock Solutions for DAPI

The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

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References for DAPI

References are publications that support the biological activity of the product.

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301

If you know of a relevant reference for DAPI, please let us know.

Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, dpi, dyes, apoptosis, staining, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience

Citations for DAPI

Citations are publications that use Tocris products.

Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? Please let us know.

Reviews for DAPI

Average Rating: 4.5 (Based on 2 Reviews.)

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DAPI staining of PC3 cells to detect apoptosis.
By Anonymous on 12/13/2018
Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.

review image

Excellent counter-stain....
By Purnima Gupta on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

Used as counter stain in IHC

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

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