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DAPI

Cat. No. 5748 2 Reviews Submit a Review Datasheet / COA / SDS
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Cat.No. 5748 - DAPI | C16H15N5.2HCl | CAS No. 28718-90-3
Description: Fluorescent DNA stain
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews (2)
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.

Technical Data

M. Wt 350.25
Formula C16H15N5.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 28718-90-3
PubChem ID 160166
InChI Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 35.02 100
water 3.5 10 with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301

If you know of a relevant reference for DAPI, please let us know.

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Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Stains, 5748, Tocris Bioscience

Citations for DAPI

Citations are publications that use Tocris products.

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Reviews for DAPI

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DAPI staining of PC3 cells to detect apoptosis.
By Anonymous on 12/13/2018
Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.

review image
Excellent counter-stain....
By Purnima Gupta on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

Used as counter stain in IHC

Literature in this Area

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