Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewFluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.
λabs | 350 nm |
---|---|
λem | 470 nm |
Application | Flow cytometry |
M. Wt | 350.25 |
Formula | C16H15N5.2HCl |
Storage | Store at -20°C |
Purity | ≥98% (HPLC) |
CAS Number | 28718-90-3 |
PubChem ID | 160166 |
InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
Smiles | N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 35.02 | 100 | |
water | 3.5 | 10 with gentle warming |
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
If you know of a relevant reference for DAPI, please let us know.
Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience
Citations are publications that use Tocris products.
Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? Please let us know.
Average Rating: 4.5 (Based on 2 Reviews.)
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Used as counter stain in IHC
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes