DAPI

Pricing Availability Delivery Time Qty
Cat.No. 5748 - DAPI | C16H15N5.2HCl | CAS No. 28718-90-3
Description: Fluorescent DNA stain
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.

Technical Data

M. Wt 350.25
Formula C16H15N5.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 28718-90-3
PubChem ID 160166
InChI Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 35.02 100
water 3.5 10mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301


If you know of a relevant reference for DAPI, please let us know.

View Related Products by Target

Keywords: DAPI, supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Dyes, Tocris Bioscience

Citations for DAPI

Citations are publications that use Tocris products.

Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for DAPI

Protocols

TODO: Add Protocols