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DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.
DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.
DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.
RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||3.5||10 with gentle warming|
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.86 mL||14.28 mL||28.55 mL|
|5 mM||0.57 mL||2.86 mL||5.71 mL|
|10 mM||0.29 mL||1.43 mL||2.86 mL|
|50 mM||0.06 mL||0.29 mL||0.57 mL|
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
Chazotte (2011) Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 1 prot5556 PMID: 21205856
Kapuscinski (1995) DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 70 220 PMID: 858020
Sandell et al (2018) DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 10 prot094029 PMID: 30275072
If you know of a relevant reference for DAPI, please let us know.
Keywords: DAPI, DAPI supplier, fluorescent, DNA, stains, cell, permeable, dpi, dyes, apoptosis, staining, nuclear, counterstain, flow, cytometry, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience
Citations are publications that use Tocris products.
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DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Used as counter stain in IHC
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: