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Description: Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95% (HPLC)
Biological Activity for DAPI
DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.
Scientific Data
Use of DAPI Stain as Nuclear Counterstain in HeLa Cells 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid dyes 520, 570 and 650. Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).
Use of DAPI Stain as Nuclear Counterstain in HeLa Cells for DAPI
Optical Data for DAPI
| Emission Color | Blue |
|---|---|
| λabs | 350 nm |
| λem | 470 nm |
| Application | Flow cytometry, ISH |
Technical Data for DAPI
| M. Wt | 350.25 |
| Formula | C16H15N5.2HCl |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 28718-90-3 |
| PubChem ID | 160166 |
| InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Smiles | N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DAPI
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.02 | 100 | |
| water | 3.5 | 10 with gentle warming |
Preparing Stock Solutions for DAPI
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
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Product Datasheets for DAPI
References for DAPI
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
Chazotte (2011) Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 1 prot5556 PMID: 21205856
Kapuscinski (1995) DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 70 220 PMID: 858020
Sandell et al (2018) DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 10 prot094029 PMID: 30275072
If you know of a relevant reference for DAPI, please let us know.
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Keywords: DAPI, DAPI supplier, fluorescent, DNA, stains, cell, permeable, dpi, dyes, apoptosis, staining, nuclear, counterstain, flow, cytometry, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience
Citations for DAPI
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Reviews for DAPI
Average Rating: 4.5 (Based on 2 Reviews.)
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DAPI staining of PC3 cells to detect apoptosis.
By
Anonymous on 12/13/2018
Species: Human
DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Excellent counter-stain....
By
Purnima Gupta on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain
Used as counter stain in IHC
Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals