DAPI
Biological Activity
Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.
Technical Data
| M. Wt | 350.25 |
| Formula | C16H15N5.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 28718-90-3 |
| PubChem ID | 160166 |
| InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Smiles | N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.02 | 100 | |
| water | 3.5 | 10 with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
If you know of a relevant reference for DAPI, please let us know.
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Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Stains, 5748, Tocris Bioscience
Citations for DAPI
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Reviews for DAPI
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Used as counter stain in IHC
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