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Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.
Optical Data for DAPI
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||3.5||10 with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.86 mL||14.28 mL||28.55 mL|
|5 mM||0.57 mL||2.86 mL||5.71 mL|
|10 mM||0.29 mL||1.43 mL||2.86 mL|
|50 mM||0.06 mL||0.29 mL||0.57 mL|
References are publications that support the biological activity of the product.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
If you know of a relevant reference for DAPI, please let us know.
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Keywords: DAPI, DAPI supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience
Citations for DAPI
Citations are publications that use Tocris products.
Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? Please let us know.
Reviews for DAPI
Average Rating: 4.5 (Based on 2 Reviews.)
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DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Used as counter stain in IHC
Literature in this Area
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New Product Guide [Spring/Summer 2019]
Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.
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