DAPI
Biological Activity
Fluorescent DNA stain. Binds to AT-rich regions of DNA. Cell permeable at high concentrations.
Technical Data
| M. Wt | 350.25 |
| Formula | C16H15N5.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 28718-90-3 |
| PubChem ID | 160166 |
| InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Smiles | N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.02 | 100 | |
| water | 3.5 | 10mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 350.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112
Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301
If you know of a relevant reference for DAPI, please let us know.
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Keywords: DAPI, supplier, fluorescent, DNA, stain, cell, permeable, Fluorescent, Dyes, Tocris Bioscience
Citations for DAPI
Citations are publications that use Tocris products.
Currently there are no citations for DAPI. Do you know of a great paper that uses DAPI from Tocris? If so please let us know.
Literature in this Area
New Product Guide (Spring/Summer 2017)New
Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.
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