Rho GTPases

Rho GTPases regulate many aspects of actin dynamics and are involved in processes that are dependent on changes to cell morphology and movement, including phagocytosis, mitosis and wound healing. They also regulate neuron dendrite spine growth, a key feature of synaptic plasticity that underlies learning and memory.

Products
Background
Literature
Gene Data

Inhibitors

Cat. No. Product Name / Activity
6701 1A-116
Rac1 inhibitor; blocks Rac1-P-Rex1 interaction
5050 CASIN
Cdc42 inhibitor
5233 CCG 1423
Rho/SRF pathway inhibitor; induces intermediate mesoderm differentiation from ESCs
4568 CPYPP
DOCK2-Rac1 interaction inhibitor
6248 EHop 016
Rac inhibitor
3872 EHT 1864
Potent Rac family inhibitor
3792 ITX 3
Selective TrioN RhoGEF activity inhibitor
6382 MAIT 203
APC-Asef (RhoGEF4) interaction inhibitor
4266 ML 141
Selective Cdc42 Rho family inhibitor
2161 NSC 23766
Selective inhibitor of Rac1-GEF interaction; antioncogenic
2221 Rac1 Inhibitor W56
Selective inhibitor of Rac1-GEF interaction
5003 Rhosin hydrochloride
Rho inhibitor
4794 ZCL 278
Cdc42 inhibitor

Controls

Cat. No. Product Name / Activity
2222 Rac1 Inhibitor F56, control peptide
Control peptide version of Rac1 Inhibitor W56 (Cat. No. 2221)

Other

Cat. No. Product Name / Activity
3715 Narciclasine
Stimulates RhoA activation; antiproliferative agent that slows cell cycle progression

Rho GTPases regulate many aspects of actin dynamics and are involved in processes that are dependent on changes to cell morphology and movement, including phagocytosis, mitosis and wound healing. To enable cells to respond to external cues, these processes require rapidly activated and spatio-temporally regulated signaling networks, of which Rho GTPases are a key component.

Three Rho GTPases have been widely studied: Cdc42, Rac1 and RhoA. For these enzymes, over 60 targets have been identified including Rho-Kinases (ROCKs), phospholipases and ion channels. As with other small GTPases, the activity of Rho GTPases is regulated by GEFs and GAPs. Inactive Rho GTPases are sequestered to the cytosol by Rho-specific guanine nucleotide dissociation inhibitors (GDIs), preventing their membrane association. Of the 20 Rho family members, 10 have been identified as being constitutively bound to GTP and so are constitutively active and have alternative regulation mechanisms.

Neuronal dendrite spine growth is dependent on Rho GTPases. This process is key to synaptic plasticity and long-term potentiation/depression, which underly learning and memory. RhoA and Cdc42 are activated by CamKII, which is activated by the influx of Ca2+ through NMDA receptors upon glutamate binding.

External sources of pharmacological information for Rho GTPases :

    Literature for Rho GTPases

    Tocris offers the following scientific literature for Rho GTPases to showcase our products. We invite you to request* or download your copy today!

    *Please note that Tocris will only send literature to established scientific business / institute addresses.


    New Product Guide

    New Product Guide (Fall/Winter 2018/2019)

    Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.

    • 7-TM Receptors
    • Enzymes
    • Enzyme-Linked Receptors
    • Ion Channels
    • Nuclear Receptors
    • Transporters
    • Chemogenetics
    • Epigenetics
    • Fluorescent Imaging
    • PROTACs
    • Signal Transduction
    • Stem Cells

    Rho GTPases Gene Data

    Gene Species Gene Symbol Gene Accession No. Protein Accession No.
    CDC42 Human CDC42 NM_001791 P60953
    Mouse Cdc42 NM_009861 P60766
    Rat Cdc42 NM_080689 P21575
    Rac1 Human RAC1 NM_018890 P63000
    Mouse Rac1 NM_001347530 P63001
    Rat Rac1 NM_134366 NP_599193
    RHOA Human RHOA NM_001664 P61586
    Mouse Rhoa NM_016802 Q9QUI0
    Rat Rhoa NM_057132 NP_476473