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Submit ReviewRhobo6 ECM Dye New
Description: Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues
Chemical Name: (E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate
Purity: ≥90% (HPLC)
Biological Activity for Rhobo6 ECM Dye
Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra.
Used for: labeling the ECM in live tissues.
Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.
Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Rhobo6 ECM Dye
| λabs | 522 nm |
|---|---|
| λem | 547 nm |
| Extinction Coefficient (ε) | 66,400 M-1cm-1 |
| Quantum Yield (φ) | 0.61 |
| Cell Permeable | No |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Rhobo6 ECM Dye
| M. Wt | 756.26 |
| Formula | C35H28B2N2O9.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | RPHZIIQRPDDKIQ-OWCLOVKRSA-N |
| Smiles | FC(F)(C(O)=O)F.OB(O)C(C=CC=C1)=C1CNC(C=C2)=CC(O3)=C2C(C(C=C(C=C4)C(O)=O)=C4C([O-])=O)=C(C=C/5)C3=CC5=[NH+]/CC6=CC=CC=C6B(O)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Rhobo6 ECM Dye
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.56 | 10 |
Preparing Stock Solutions for Rhobo6 ECM Dye
The following data is based on the product molecular weight 756.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.22 mL | 66.11 mL | 132.23 mL |
| 0.5 mM | 2.64 mL | 13.22 mL | 26.45 mL |
| 1 mM | 1.32 mL | 6.61 mL | 13.22 mL |
| 5 mM | 0.26 mL | 1.32 mL | 2.64 mL |
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Product Datasheets for Rhobo6 ECM Dye
References for Rhobo6 ECM Dye
References are publications that support the biological activity of the product.
Fiore et al (2025) Live imaging of the extracellular matrix with a glycan-binding fluorophore. Nat.Methods 22 1070 PMID: 39915692
If you know of a relevant reference for Rhobo6 ECM Dye, please let us know.
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