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Description: Glutathione (GSH) precursor; maintains stem cell function in culture.
Chemical Name: (2R)-2-Acetamido-3-sulfanylpropanoic acid
Purity: ≥98% (HPLC)

Biological Activity for N-Acetylcysteine

N-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.

Technical Data for N-Acetylcysteine

M. Wt 163.2
Formula C5H9NO3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 616-91-1
PubChem ID 12035
Smiles [C@@H](NC(C)=O)(C(O)=O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for N-Acetylcysteine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 8.16 50
water 8.16 50

Preparing Stock Solutions for N-Acetylcysteine

The following data is based on the product molecular weight 163.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 12.25 mL 61.27 mL 122.55 mL
2.5 mM 2.45 mL 12.25 mL 24.51 mL
5 mM 1.23 mL 6.13 mL 12.25 mL
25 mM 0.25 mL 1.23 mL 2.45 mL

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Product Datasheets for N-Acetylcysteine

References for N-Acetylcysteine

References are publications that support the biological activity of the product.

Cattaneo et al (2019) Tumor organoid-T-cell coculture systems. Nat.Protoc. 15 15 PMID: 31853056

Broutier et al (2016) Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat.Protoc. 11 1724 PMID: 27560176

Balboa et al (2022) Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. Nat.Biotechnol. 40 1042 PMID: 35241836

Moiseeva et al (2023) Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature 613 169 PMID: 36544018

Geiler et al (2009) N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus. Biochem.Pharmacol. 79 413 PMID: 19732754

If you know of a relevant reference for N-Acetylcysteine, please let us know.

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Citations for N-Acetylcysteine

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