N-Acetylcysteine

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Glutathione (GSH) precursor; maintains stem cell function in culture.
Chemical Name: (2R)-2-Acetamido-3-sulfanylpropanoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews

Biological Activity for N-Acetylcysteine

N-Acetylcysteine is a cell-permeable antioxidant and a precursor of reduced glutathione (GSH) (Cat. No. 5219), with anti-inflammatory, mucolytic, and antiviral activities. N-Acetylcysteine can be used in organoid culture media for non-small cell lung and colorectal cancer organoids, expansion media for mouse/human liver and pancreas 3D organoids and LWRN media for colonic 3D organoids from human biopsies. It is a component of S7 medium in the optimized protocol for generation of functional stem cell-derived islets (SC-islets). N-Acetylcysteine can be orally administered.

Technical Data for N-Acetylcysteine

M. Wt 163.2
Formula C5H9NO3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 616-91-1
PubChem ID 12035
InChI Key PWKSKIMOESPYIA-BYPYZUCNSA-N
Smiles [C@@H](NC(C)=O)(C(O)=O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for N-Acetylcysteine

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 8.16 50
water 8.16 50

Preparing Stock Solutions for N-Acetylcysteine

The following data is based on the product molecular weight 163.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 12.25 mL 61.27 mL 122.55 mL
2.5 mM 2.45 mL 12.25 mL 24.51 mL
5 mM 1.23 mL 6.13 mL 12.25 mL
25 mM 0.25 mL 1.23 mL 2.45 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for N-Acetylcysteine

References for N-Acetylcysteine

References are publications that support the biological activity of the product.

Cattaneo et al (2019) Tumor organoid-T-cell coculture systems. Nat.Protoc. 15 15 PMID: 31853056

Broutier et al (2016) Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat.Protoc. 11 1724 PMID: 27560176

Balboa et al (2022) Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. Nat.Biotechnol. 40 1042 PMID: 35241836

Moiseeva et al (2023) Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature 613 169 PMID: 36544018

Geiler et al (2009) N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus. Biochem.Pharmacol. 79 413 PMID: 19732754


If you know of a relevant reference for N-Acetylcysteine, please let us know.

Keywords: N-Acetylcysteine, N-Acetylcysteine supplier, cell, permeable, antioxidant, glutathione, precursor, GSH, organoids, stem, cells, differentiation, mucolytic, antiviral, anti-inflammatory, NAC, nacetylcysteine, Antioxidants, Neural, Stem, Cells, Organoids, Cancer, ESCs, and, iPSC, Mesenchymal, Other, Differentiation, Products, Influenza, Viruses, 7874, Tocris Bioscience

Citations for N-Acetylcysteine

Citations are publications that use Tocris products.

Currently there are no citations for N-Acetylcysteine. Do you know of a great paper that uses N-Acetylcysteine from Tocris? Please let us know.

Reviews for N-Acetylcysteine

There are currently no reviews for this product. Be the first to review N-Acetylcysteine and earn rewards!

Have you used N-Acetylcysteine?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review