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Description: PARP-1 inhibitor
Chemical Name: Pyridine-3-carboxamide
Purity: ≥99% (HPLC)
Citations (6)
Protocols (1)
Literature (2)

Biological Activity for Nicotinamide

Nicotinamide is an anti-inflammatory agent. Inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. NAD+ precursor. Promotes differentiation of mesenchymal stem cells to insulin producing cells when used in combination with growth factors and high glucose concentration. Nicotinamide also acts as a SIRT1 inhibitor and promotes the expansion of hematopoietic progenitor cells

Nicotinamide synthesized to Ancillary Material Grade also available.

For more information about how Nicotinamide may be used, see our protocol:

3D Culture of Lung Alveolar Cells

Compound Libraries for Nicotinamide

Nicotinamide is also offered as part of the Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Nicotinamide

M. Wt 122.12
Formula C6H6N2O
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 98-92-0
PubChem ID 936
Smiles NC(C1=CC=CN=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Nicotinamide

Solvent Max Conc. mg/mL Max Conc. mM
water 12.21 100

Preparing Stock Solutions for Nicotinamide

The following data is based on the product molecular weight 122.12. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 8.19 mL 40.94 mL 81.89 mL
5 mM 1.64 mL 8.19 mL 16.38 mL
10 mM 0.82 mL 4.09 mL 8.19 mL
50 mM 0.16 mL 0.82 mL 1.64 mL

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References for Nicotinamide

References are publications that support the biological activity of the product.

Suzuki et al (2010) Protective effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediated uptake. Life.Sci. 86 676 PMID: 20188745

Cimadamore et al (2009) Nicotinamide rescues human embryonic stem cell-derived neuroectoderm from parthanatic cell death. Stem Cells. 27 1772 PMID: 19544437

Czubak et al (2014) A modified method of Ins producing cells' generation from bone marrow-derived mesenchymal stem cells. J.Diabetes Res. 2014 628591 PMID: 25405207

Sato et al (2015) SnapShot: Growing organoids from stem cells. Cell 161 1700 PMID: 26091044

Bartfeld et al (2015) In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 148 126 PMID: 25307862

Jung et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 17 1227 PMID: 21892181

Boj et al (2015) Organoid models of human and mouse ductal pancreatic cancer. Cell 160 324 PMID: 25557080

Peled et al (2012) Nicotinamide, a SIRT1 inhibitor, inhibits differentiation and facilitates expansion of hematopoietic progenitor cells with enhanced bone marrow homing and engraftment. Exp.Hematol. 40 342 PMID: 22198152

If you know of a relevant reference for Nicotinamide, please let us know.

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Keywords: Nicotinamide, Nicotinamide supplier, Nicotinamide, inhibits, poly(ADP-ribose), polymerase-1, PARP-1, inhibitors, anti-inflammatory, organoids, hematopoietic, haematopoietic, stem, cells, HSC, progenitors, SIRT1, Poly(ADP-ribose), Polymerase, Mesenchymal, Stem, Cells, Organoids, Hematopoietic, Class, III, HDACs, (Sirtuins), 4106, Tocris Bioscience

6 Citations for Nicotinamide

Citations are publications that use Tocris products. Selected citations for Nicotinamide include:

Steven L et al (2022) INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury. Nat Commun 13 6037 PMID: 36229429

Thomas C et al (2019) Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity. Cell Stem Cell 25 103-119.e6 PMID: 31155484

Emanuela et al (2020) Cell-type-specific regulation of neuronal intrinsic excitability by macroautophagy. Elife 9 PMID: 31913125

Jun et al (2017) Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell 169 243-257.e25 PMID: 28388409

Do you know of a great paper that uses Nicotinamide from Tocris? Please let us know.

Reviews for Nicotinamide

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Protocols for Nicotinamide

The following protocol features additional information for the use of Nicotinamide (Cat. No. 4106).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:

  • Cell Cycle and Mitosis
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Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.