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Bleomycin sulfate

Cat. No. 7147 Submit a Review Datasheet / COA / SDS
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Description: DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis
Alternative Names: NSC125066
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Bleomycin sulfate

Bleomycin sulfate is a glycopeptide antibiotic produced by Streptomyces verticillus. It inhibits DNA synthesis and causes single and double-strand breaks at specific base sequences in intracellular DNA of bacterial and mammalian cells. It also acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. It is an anticancer agent for squamous cell carcinomas (SCC) with IC50 = 4 nM in UT-SCC-19A cells.

In vivo, Bleomycin sulfate induces pulmonary inflammation and fibrosis in lung injury models (mice).

Bleomycin sulfate contains two main components:

1) Bleomycin A2 hemisulfate, C55H84N17O21S3.HSO4, molecular weight = 1512.62 Da

2) Bleomycin B2, C55H84N20O21S2, molecular weight = 1425.52 Da.

The exact proportions vary from batch to batch and can be found on the relevant certificate of analysis.

Technical Data for Bleomycin sulfate

M. Wt 1512.6
Formula C55H84N17O21S3.HSO4 (for A2)
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 9041-93-4
PubChem ID 9812642
InChI Key WUIABRMSWOKTOF-OYALTWQYSA-N
Smiles O=S(O)([O-])=O.O=C(N[C@@H]([C@@H](O[C@H]1[C@@H](O[C@@H]2[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O2)[C@@H](O)[C@H](O)[C@H](CO)O1)C3=CN=CN3)C(N[C@@H]([C@H]([C@@H](C(N[C@H](C(NCCC4=NC(C5=NC(C(NCCC[S+](C)C)=O)=CS5)=CS4)=O)[C@@H](C)O)=O)C)O)C)=O)C6=NC([C@@H](NC[C@@H](C(N)=O)N)CC(N)=O)=NC(N)=C6C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Bleomycin sulfate

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20 14
water 20 14

Preparing Stock Solutions for Bleomycin sulfate

The following data is based on the product molecular weight 1512.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.14 mM 4.72 mL 23.61 mL 47.22 mL
0.7 mM 0.94 mL 4.72 mL 9.44 mL
1.4 mM 0.47 mL 2.36 mL 4.72 mL
7 mM 0.09 mL 0.47 mL 0.94 mL

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Product Datasheets for Bleomycin sulfate

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for Bleomycin sulfate

References are publications that support the biological activity of the product.

D'Andrea et al (1978) Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin. Proc.Natl.Acad.Sci.USA 75 3608 PMID: 80799

Liu et al (2018) Telomere shortening activates TGF-β/Smads signaling in lungs and enhances both lipopolysaccharide and bleomycin-induced pulmonary fibrosis. Pharmacol.Sin 39 1735 PMID: 29925920

Lin et al (2015) Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiol.Rep 3 PMID: 26702074

Jääskelä-Saari et al (1997) Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Oto-Laryngologica 117 241 PMID: 9288321

Banerjee et al (2012) Characterization of lung stem cell niches in a mouse model of bleomycin-induced fibrosis. Stem Cell Res.Ther. 3 PMID: 22643035

If you know of a relevant reference for Bleomycin sulfate, please let us know.

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Citations for Bleomycin sulfate

Citations are publications that use Tocris products.

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Literature in this Area

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