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Description: Multipurpose HEPES buffer; used in cell culture
Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Purity: ≥99.5%
Citations (1)
Reviews (3)
Protocols (1)

Biological Activity for HEPES

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

For more information about how HEPES may be used, see our protocol:

3D Culture of Lung Alveolar Cells

Technical Data for HEPES

M. Wt 238.3
Formula C8H18N2O4S
Storage Store at RT
Purity ≥99.5%
CAS Number 7365-45-9
PubChem ID 23830
Smiles OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for HEPES

Solvent Max Conc. mg/mL Max Conc. mM
water 238.3 1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
10 mM 0.42 mL 2.1 mL 4.2 mL
50 mM 0.08 mL 0.42 mL 0.84 mL
100 mM 0.04 mL 0.21 mL 0.42 mL
500 mM 0.01 mL 0.04 mL 0.08 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for HEPES

Certificate of Analysis / Product Datasheet
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References for HEPES

References are publications that support the biological activity of the product.

Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950

Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651

If you know of a relevant reference for HEPES, please let us know.

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Keywords: HEPES, HEPES supplier, Multipurpose, buffer, Reagents, cell, culture, ph, Buffers,, Solvents, and, Solutions, 3173, Tocris Bioscience

1 Citation for HEPES

Citations are publications that use Tocris products. Selected citations for HEPES include:

Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543

Do you know of a great paper that uses HEPES from Tocris? Please let us know.

Reviews for HEPES

Average Rating: 4.3 (Based on 3 Reviews.)

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HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging..
By Anonymous on 07/02/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.

Good buffer.
By Anonymous on 08/05/2018
Assay Type: Ex Vivo
Species: Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.

HEPES to buffer pH.
By Anonymous on 01/31/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Brain slice

HEPES used in solutions, good pH buffer.

Protocols for HEPES

The following protocol features additional information for the use of HEPES (Cat. No. 3173).