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Biological Activity for HEPES
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
For more information about how HEPES may be used, see our protocol:3D Culture of Lung Alveolar Cells
Technical Data for HEPES
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HEPES
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for HEPES
The following data is based on the product molecular weight 238.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|10 mM||0.42 mL||2.1 mL||4.2 mL|
|50 mM||0.08 mL||0.42 mL||0.84 mL|
|100 mM||0.04 mL||0.21 mL||0.42 mL|
|500 mM||0.01 mL||0.04 mL||0.08 mL|
References for HEPES
References are publications that support the biological activity of the product.
Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950
Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651
If you know of a relevant reference for HEPES, please let us know.
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1 Citation for HEPES
Citations are publications that use Tocris products. Selected citations for HEPES include:
Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543
Do you know of a great paper that uses HEPES from Tocris? Please let us know.
Reviews for HEPES
Average Rating: 4.6 (Based on 5 Reviews.)
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HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.
Used at 20 mmol/L in DMEM buffer for primary cell culture
Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.
Additive in cell culture media
HEPES used in solutions, good pH buffer.