HEPES

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Cat.No. 3173 - HEPES | C8H18N2O4S | CAS No. 7365-45-9
Description: Multipurpose buffer
Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Purity: ≥99.5%
Datasheet
Citations (1)
Reviews (3)
Literature

Biological Activity

Multi purpose buffer used in biological research.

Technical Data

M. Wt 238.3
Formula C8H18N2O4S
Storage Store at RT
Purity ≥99.5%
CAS Number 7365-45-9
PubChem ID 23830
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
Smiles OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 238.3 1000

Preparing Stock Solutions

The following data is based on the product molecular weight 238.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.2 mL 20.98 mL 41.96 mL
5 mM 0.84 mL 4.2 mL 8.39 mL
10 mM 0.42 mL 2.1 mL 4.2 mL
50 mM 0.08 mL 0.42 mL 0.84 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950

Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651


If you know of a relevant reference for HEPES, please let us know.

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Keywords: HEPES, HEPES supplier, Multipurpose, buffer, Reagents, Buffers,, Solvents, and, Solutions, 3173, Tocris Bioscience

1 Citation for HEPES

Citations are publications that use Tocris products. Selected citations for HEPES include:

Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543


Do you know of a great paper that uses HEPES from Tocris? Please let us know.

Reviews for HEPES

Average Rating: 4.7 (Based on 3 Reviews.)

5 Star
67%
4 Star
33%
3 Star
0%
2 Star
0%
1 Star
0%

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Good buffer
By Anonymous on 08/05/2018
Assay Type: Ex Vivo
Species: Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.


Media ingredient
By Anonymous on 05/01/2018
Species: Mouse

Additive in cell culture media


HEPES to buffer pH
By Anonymous on 01/31/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Brain slice

HEPES used in solutions, good pH buffer.


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

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