DAPT

Cat. No. 2634

DAPT C23H26F2N2O4 [208255-80-5]

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Chemical Name: N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester

Biological Activity

Inhibitor of γ-secretase; causes a reduction in Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. Does not affect APPα and APPβ levels. Blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC). Activity causes ESCs to commit to neuronal differentiation.

Technical Data

M.Wt:
432.46
Formula:
C23H26F2N2O4
Solubility:
Soluble to 100 mM in DMSO
Purity:
>99 %
Storage:
Store at +4°C
CAS No:
208255-80-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Safety Data Sheet

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Crawford and Roelink (2007) The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn. 236 886. PMID: 17295317.

De Smedt et al (2005) Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment. Blood 106 3498. PMID: 16030192.

Dovey et al (2001) Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J.Neurochem. 76 173. PMID: 11145990.

If you know of a relevant reference for this product please let us know.

Citations are publications that use Tocris products. Selected citations for DAPT include:

Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89. PMID: 26655496.

Edri et al (2015) Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6 6500. PMID: 25799239.

Kerr et al (2015) A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis. Angiogenesis 18 209. PMID: 25557927.

Wang et al (2015) Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions. Stem Cell Res Ther 6 223. PMID: 26564165.

Greife et al (2014) Canonical Notch signalling is inactive in urothelial carcinoma. BMC Cancer 14 628. PMID: 25167871.

Hammond et al (2014) Astrocyte-derived endothelin-1 inhibits remyelination through notch activation. Neuron 81 588. PMID: 24507193.

Díaz et al (2013) Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol 201 279. PMID: 23589494.

Du et al (2013) Inflammation-mediated notch signaling skews fanconi anemia hematopoietic stem cell differentiation. J Immunol 191 2806. PMID: 23926327.

Kirkeby et al (2013) Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci 6 64. PMID: 23316134.

Sundvik et al (2013) Presenilin1 regulates histamine neuron development and behavior in zebrafish, danio rerio. J Neurosci 33 1589. PMID: 23345232.

Boulter et al (2012) Macrophage-derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nat Med 18 572. PMID: 22388089.

Campos et al (2012) Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration. J Virol 86 4169. PMID: 22345461.

Hu et al (2012) Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling. Cancer Res 72 3873. PMID: 22665268.

Berndt et al (2011) Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/β-catenin signaling. J Cell Biol 194 737. PMID: 21875946.

Kolegraff et al (2011) Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines. Cell Adh Migr 5 306. PMID: 21715983.

Do you know of a great paper that uses DAPT from Tocris? If so please let us know.show all ▼show less ▲

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Keywords: DAPT, supplier, γ-secretase, gamma-secretase, inhibitors, inhibits, reduces, Aβ4, β42, beta42, levels, Proteinases, Proteases, β-Amyloid, beta-Amyloid, b-amyloid, Precursor, Protein, APP, beta, Peptides, amyloidbeta, amyloidb, amyloidβ, organoids, Tocris Bioscience, γ-Secretase Inhibitor products

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