Cat. No. 2634

DAPT C23H26F2N2O4 [208255-80-5]

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Chemical Name: N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester

Biological Activity

Inhibitor of γ-secretase; causes a reduction in Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. Does not affect APPα and APPβ levels. Blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC). Activity causes ESCs to commit to neuronal differentiation.

Technical Data

Soluble to 100 mM in DMSO
>99 %
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

Certificate of Analysis / Product Datasheet
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References are publications that support the products' biological activity.

Crawford and Roelink (2007) The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn. 236 886. PMID: 17295317.

De Smedt et al (2005) Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment. Blood 106 3498. PMID: 16030192.

Dovey et al (2001) Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J.Neurochem. 76 173. PMID: 11145990.

If you know of a relevant reference for DAPT please let us know.

Citations are publications that use Tocris products. Selected citations for DAPT include:

Schumacher (2017) ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation. Cell Death Dis 8 e2622. PMID: 28230865.

Campbell et al (2016) Canonical Notch signaling plays an instructive role in auditory supporting cell development. Zoological Lett 6 19484. PMID: 26786414.

Guest et al (2016) Notch3 drives development and progression of cholangiocarcinoma Proc Natl Acad Sci U S A. 113 12250. PMID: 27791012.

Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89. PMID: 26655496.

Edri et al (2015) Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6 6500. PMID: 25799239.

Gomez et al (2015) Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. Sci Rep 10 e0133862. PMID: 26288179.

Kerr et al (2015) A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis. Angiogenesis 18 209. PMID: 25557927.

Onai et al (2015) On the origin of vertebrate somites. Blood 1 33. PMID: 26613046.

Wang et al (2015) Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions. Stem Cell Res Ther 6 223. PMID: 26564165.

Gioia et al (2014) Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1. Anticancer Res 11 1105. PMID: 25483045.

Greife et al (2014) Canonical Notch signalling is inactive in urothelial carcinoma. BMC Cancer 14 628. PMID: 25167871.

Hammond et al (2014) Astrocyte-derived endothelin-1 inhibits remyelination through notch activation. Neuron 81 588. PMID: 24507193.

Díaz et al (2013) Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol 201 279. PMID: 23589494.

Du et al (2013) Inflammation-mediated notch signaling skews fanconi anemia hematopoietic stem cell differentiation. J Immunol 191 2806. PMID: 23926327.

Kirkeby et al (2013) Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci 6 64. PMID: 23316134.

Shah et al (2013) The role of Notch and γ-secretase inhibition in an ovarian cancer model. J Neurosci 33 801. PMID: 23482747.

Sundvik et al (2013) Presenilin1 regulates histamine neuron development and behavior in zebrafish, danio rerio. J Neurosci 33 1589. PMID: 23345232.

Bejjani and Hammarlund (2012) Notch signaling inhibits axon regeneration. PLoS One 73 268. PMID: 22284182.

Boulter et al (2012) Macrophage-derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nat Med 18 572. PMID: 22388089.

Campos et al (2012) Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration. J Virol 86 4169. PMID: 22345461.

Hu et al (2012) Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling. Cancer Res 72 3873. PMID: 22665268.

Scheppke et al (2012) Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane. Cell Stem Cell 119 2149. PMID: 22134168.

Valdez et al (2012) Notch and TGFβ form a reciprocal positive regulatory loop that suppresses murine prostate basal stem/progenitor cell activity. RNA Biol 11 676. PMID: 23122291.

Berndt et al (2011) Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/β-catenin signaling. J Cell Biol 194 737. PMID: 21875946.

Kolegraff et al (2011) Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines. Cell Adh Migr 5 306. PMID: 21715983.

Kim et al (2010) Group II metabotropic glutamate receptor stimulation triggers production and release of Alzheimer's amyloid(β)42 from isolated intact nerve terminals. Neuron 30 3870. PMID: 20237257.

Do you know of a great paper that uses DAPT from Tocris? If so please let us know.show all ▼show less ▲

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Keywords: DAPT, supplier, γ-secretase, gamma-secretase, inhibitors, inhibits, reduces, Aβ4, β42, beta42, levels, Proteinases, Proteases, β-Amyloid, beta-Amyloid, b-amyloid, Precursor, Protein, APP, beta, Peptides, amyloidbeta, amyloidb, amyloidβ, organoids, Tocris Bioscience, γ-Secretase Inhibitor products

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