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Description: Smo receptor agonist
Chemical Name: 9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine
Purity: ≥98% (HPLC)
Citations (8)
Reviews (3)
Literature (2)

Biological Activity for Purmorphamine

Purmorphamine is a smoothened (Smo) receptor agonist (EC50 ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.

Scientific Data

Applications of Purmorphamine in Neurons Derived from hiPSCs Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Compound Libraries for Purmorphamine

Purmorphamine is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Purmorphamine

M. Wt 520.62
Formula C31H32N6O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 483367-10-8
PubChem ID 5284329
Smiles C1(OC4=CC=CC5=C4C=CC=C5)=NC(NC3=CC=C(N6CCOCC6)C=C3)=C2C(N(C7CCCCC7)C=N2)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Purmorphamine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 52.06 100

Preparing Stock Solutions for Purmorphamine

The following data is based on the product molecular weight 520.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.92 mL 9.6 mL 19.21 mL
5 mM 0.38 mL 1.92 mL 3.84 mL
10 mM 0.19 mL 0.96 mL 1.92 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

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Product Datasheets for Purmorphamine

Certificate of Analysis / Product Datasheet
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References for Purmorphamine

References are publications that support the biological activity of the product.

Wu et al (2004) Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem.Biol. 11 1229 PMID: 15380183

Sinha et al (2006) Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat.Chem.Biol. 2 29 PMID: 16408088

Wu et al (2002) A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J.Am.Chem.Soc. 124 14520 PMID: 12465946

Sinha and Chen et al (2006) Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat.Chem.Biol. 2 29 PMID: 16408088

If you know of a relevant reference for Purmorphamine, please let us know.

View Related Products by Product Action

View all Smoothened Receptor Agonists

Keywords: Purmorphamine, Purmorphamine supplier, Smoothened, Smo, receptors, agonists, differentiation, multipotent, mesenchymal, progenitor, cells, osteoblasts, osteogenic, Receptors, Mesenchymal, Stem, Cells, Osteogenic, 4551, Tocris Bioscience

8 Citations for Purmorphamine

Citations are publications that use Tocris products. Selected citations for Purmorphamine include:

Sommer et al (2018) Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease. Cell Stem Cell. 23 123 PMID: 29979986

Rajamani et al (2018) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell Stem Cell 22 698 PMID: 29681516

Yuan et al (2016) Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. J Immunol 7 11024 PMID: 26996322

Hackland et al (2019) FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision. Stem Cell Reports 12 920 PMID: 31091435

Do you know of a great paper that uses Purmorphamine from Tocris? Please let us know.

Reviews for Purmorphamine

Average Rating: 5 (Based on 3 Reviews.)

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Usage Information.
By Anonymous on 07/04/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: iPSC

iPSC-derived hypothalamic neuron differentiation activation by Smoothened agonist SAG 1 μM and purmorphamine 1 μM.

From Day 2 to day 9 to direct the cells toward ventral diencephalon with regular media change every 2 days.

PMID: 29681516
review image

For differentiation of DaNs.
By Phi Anh Phan on 02/04/2020
Species: Human
Cell Line/Tissue: iPSC

Used for the differentiation of human iPSCs into dopaminergic neurons (Kriks et al.,2011).

review image

Great for differentiation of huiPSCs into DaNs!.
By Phi Anh Phan on 01/31/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC

Purmorphamine was used to differentiate human iPSCs into dopaminergic neurons with above 60% TH positive cells.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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