DAPT

GMP Version Available: DAPT
Pricing Availability   Qty
Cat.No. 2634 - DAPT | C23H26F2N2O4 | CAS No. 208255-80-5
Description: γ-secretase inhibitor; induces neuronal differentiation
Chemical Name: (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester
Purity: ≥99% (HPLC)
Datasheet
Citations (49)
Reviews (2)
Protocols (4)
Literature (4)

Biological Activity

Inhibitor of γ-secretase; causes a reduction in Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. Does not affect APPα and APPβ levels. Blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC). Activity causes ESCs to commit to neuronal differentiation.

DAPT synthesized to cGMP guidelines also available.

Compound Libraries

DAPT is also offered as part of the Tocriscreen Plus and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 432.46
Formula C23H26F2N2O4
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 208255-80-5
PubChem ID 5311272
InChI Key DWJXYEABWRJFSP-XOBRGWDASA-N
Smiles CC(C)(C)OC([C@H]([C@]2=CC=CC=C2)NC([C@H](C)NC(CC1=CC(F)=CC(F)=C1)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 43.24 100

Preparing Stock Solutions

The following data is based on the product molecular weight 432.46. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.31 mL 11.56 mL 23.12 mL
5 mM 0.46 mL 2.31 mL 4.62 mL
10 mM 0.23 mL 1.16 mL 2.31 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:

References

References are publications that support the biological activity of the product.

Dovey et al (2001) Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J.Neurochem. 76 173 PMID: 11145990

De Smedt et al (2005) Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment. Blood 106 3498 PMID: 16030192

Crawford and Roelink (2007) The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn. 236 886 PMID: 17295317

Sato et al (2015) SnapShot: Growing Organoids from Stem Cells. Cell 161 1700 PMID: 26091044

Lancaster et al (2015) Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat.Protoc. 9 2329 PMID: 25188634


If you know of a relevant reference for DAPT, please let us know.

Keywords: DAPT, DAPT supplier, γ-secretase, gamma-secretase, inhibitors, inhibits, reduces, Aβ4, β42, beta42, levels, Proteinases, Proteases, β-Amyloid, beta-Amyloid, b-amyloid, Precursor, Protein, APP, beta, Peptides, amyloidbeta, amyloidb, amyloidβ, organoids, Gamma-Secretase, Amyloid, Beta, Neural, Stem, Cells, Organoids, 2634, Tocris Bioscience

49 Citations for DAPT

Citations are publications that use Tocris products. Selected citations for DAPT include:

Xiang et al (2019) Long-term functional maintenance of primary human hepatocytes in vitro. Science 364 399 PMID: 31023926

Vila et al (2019) Quantification of human neuromuscular function through optogenetics. Theranostics 9 1232 PMID: 30867827

Kerr et al (2015) A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis. Angiogenesis 18 209 PMID: 25557927

Wang et al (2015) Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions. Stem Cell Res Ther 6 223 PMID: 26564165

Edri et al (2015) Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6 6500 PMID: 25799239

Gioia et al (2014) Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1. Anticancer Res 11 1105 PMID: 25483045

Shah et al (2013) The role of Notch and γ-secretase inhibition in an ovarian cancer model. J Neurosci 33 801 PMID: 23482747

Berndt et al (2011) Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/β-catenin signaling. J Cell Biol 194 737 PMID: 21875946

Kolegraff et al (2011) Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines. Cell Adh Migr 5 306 PMID: 21715983

Haidar et al (2019) Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Autophagy 15 1051 PMID: 30669930

Lin et al (2017) Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop. Oncotarget 8 110426 PMID: 29299159

Campbell et al (2016) Canonical Notch signaling plays an instructive role in auditory supporting cell development. Zoological Lett 6 19484 PMID: 26786414

Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89 PMID: 26655496

Onai et al (2015) On the origin of vertebrate somites. Blood 1 33 PMID: 26613046

Gomez et al (2015) Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. Sci Rep 10 e0133862 PMID: 26288179

Greife et al (2014) Canonical Notch signalling is inactive in urothelial carcinoma. BMC Cancer 14 628 PMID: 25167871

Hammond et al (2014) Astrocyte-derived endothelin-1 inhibits remyelination through notch activation. Neuron 81 588 PMID: 24507193

Wang et al (2018) The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest 128 3319 PMID: 29939162

Takeuchi et al (2018) VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush. Sci Rep 8 16443 PMID: 30401804

Sheng et al (2018) A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nat Commun 9 4047 PMID: 30279449

Kishinevsky et al (2018) HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain DA neurons. Nat Commun 9 4345 PMID: 30341316

Wang et al (2016) Efficient Generation of Hypothalamic Neurons from Human Pluripotent Stem Cells. Curr Protoc Hum Genet 90 21.5.1 PMID: 27367166

Zeltner et al (2016) Capturing the biology of disease severity in a PSC-based model of familial dysautonomia. Nat Med 22 1421 PMID: 27841875

Pavlou et al (2014) Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus. PLoS One 9 e87546 PMID: 24498133

Scheppke et al (2012) Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane. Cell Stem Cell 119 2149 PMID: 22134168

Valdez et al (2012) Notch and TGFβ form a reciprocal positive regulatory loop that suppresses murine prostate basal stem/progenitor cell activity. RNA Biol 11 676 PMID: 23122291

Kim et al (2010) Group II metabotropic glutamate receptor stimulation triggers production and release of Alzheimer's amyloid(β)42 from isolated intact nerve terminals. Neuron 30 3870 PMID: 20237257

Guest et al (2016) Notch3 drives development and progression of cholangiocarcinoma Proc Natl Acad Sci U S A. 113 12250 PMID: 27791012

Yang (2018) A biodegradable hybrid inorganic nanoscaffold for advanced stem cell therapy. Nat Commun 9 3147 PMID: 30089775

Ikram et al (2018) Co-targeting of Tiam1/Rac1 and Notch ameliorates chemoresistance against dox. in a biomimetic 3D lymphoma model. Oncotarget 9 2058 PMID: 29416753

Wang (2018) Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. Nat Med 24 647 PMID: 29632371

Fraser et al (2013) Common developmental pathways link tooth shape to regeneration. Dev Biol 377 399 PMID: 23422830

Li et al (2013) Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation. Proc Natl Acad Sci U S A 110 E1452 PMID: 23576731

Marumo et al (2013) Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci Res 75 204 PMID: 23396245

Campos et al (2012) Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration. J Virol 86 4169 PMID: 22345461

Boulter et al (2012) Macrophage-derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nat Med 18 572 PMID: 22388089

Hu et al (2012) Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling. Cancer Res 72 3873 PMID: 22665268

Bejjani and Hammarlund (2012) Notch signaling inhibits axon regeneration. PLoS One 73 268 PMID: 22284182

Calton et al (2017) Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis. PLoS Pathog 13 e1006200 PMID: 28463988

Raven et al (2017) Soluble Gamma-secretase Modulators Attenuate Alzheimer's β-amyloid Pathology and Induce Conformational Changes in Presenilin 1. EBioMedicine 24 93 PMID: 28919280

Schumacher (2017) ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation. Cell Death Dis 8 e2622 PMID: 28230865

Díaz et al (2013) Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol 201 279 PMID: 23589494

Du et al (2013) Inflammation-mediated notch signaling skews fanconi anemia hematopoietic stem cell differentiation. J Immunol 191 2806 PMID: 23926327

Kirkeby et al (2013) Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci 6 64 PMID: 23316134

Sundvik et al (2013) Presenilin1 regulates histamine neuron development and behavior in zebrafish, danio rerio. J Neurosci 33 1589 PMID: 23345232

Hsieh and Lo (2012) Jagged1 and Notch1 help edit M cell patterning in Peyer's patch follicle epithelium. Dev Comp Immunol 37 306 PMID: 22504165

Tchieu et al (2017) A modular platform for differentiation of human PSCs into all major ectodermal lineages. Cell Stem Cell 21 399 PMID: 28886367

Alsanie (2017) Specification of murine ground state pluripotent stem cells to regional neuronal populations. Sci Rep 7 16001 PMID: 29167563

Pitt et al (2017) Neuroprotective astrocyte-derived Ins/IGF-1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers. Mol Biol Cell 28 2623 PMID: 28963439


Do you know of a great paper that uses DAPT from Tocris? Please let us know.

Reviews for DAPT

Average Rating: 5 (Based on 2 Reviews.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used DAPT?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


used in human cell line cultures.
By Anonymous on 08/20/2019
Assay Type: In Vitro
Species: Human

In cell line culture of human cells


Works great..
By Anonymous on 01/04/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Induced pluripotent stem cells

I used this product for differentiation of iPSCs into dopaminergic neurons according to the protocol published in Kriks et al, 2011.I used the concentration 10uM and it works really well.


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cell Workflow

Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.