Imaging Applications

Tocris offers an extensive range of fluorescent and bioluminescent dyes, probes, indicators, buffers and more to support all your fluorescent imaging needs. We have grouped all the main dyes, counter stains and buffers according to applications below. You will also find links to the comprehensive range of antibodies from our sister brands R&D Systems™ and Novus Biologicals, including over 40,000 IHC validated antibodies.

Literature (1)

Spectra Viewer

Use our spectra viewer to interactively plan your imaging experiments. View the excitation and emission data for all common dyes including Janelia Fluor ® dyes, Cyanine Dyes (Cy Dyes), TFAX (AF) dyes and BDY (BODIPY®) dyes. Note: absorption spectra may be displayed instead of excitation spectra.

 Use our spectra viewer to plan your flow cytometry experiments

Featured Product Ranges

Janelia Fluor® Dyes

Janelia Fluor® (JF) dyes are bright, cell permeable and highly photostable small molecule fluorophores, which are especially well suited to live-cell imaging and super-resolution techniques. Janelia Fluor® dyes are supplied with a choice of reactive groups including click handles (tetrazine and azide), free acid, maleimide, and NHS ester (SE), allowing for simple biomolecule conjugation (conjugation protocols available).

Janelia Fluor® dyes are compatible with self-labeling tag systems e.g. HaloTag® and SNAP-tag®. These dyes can be used with conventional imaging techniques, such as confocal microscopy, IHC, ICC and flow cytometry, as well as super-resolution microscopy (SRM) techniques, including STED and dSTORM. Our range also includes the Janelia Fluor® photoactivatable dyes that are applicable in PALM microscopy.

The Janelia Fluor® range is ideal for multiplexing as the dyes have narrow absorption/emission spectra spanning the color spectrum from the green/yellow Janelia Fluor® 525, to the far red Janelia Fluor® 669.

For pre-conjugated options, we also offer Janelia Fluor® conjugated antibodies and custom conjugation services through Novus Biologicals, our Bio-Techne sister brand.

Application of Janelia Fluor® Dyes in Cardiac Tissue

Application of Janelia Fluor® Dyes in Cardiac Tissue - Left - displaying collagen VI in the interstitial space between muscle cells. Labeled with a primary ab against collagen VI and a secondary ab conjugated to JF549 (#6147). Scale: 50 μm. Middle - adult pig heart tissue, 10 μm thick, labeled with abs against SERCA2ATPase, displays sarcoplasmic reticulum. Top: super-resolution image (dSTORM), bottom: diffraction limited widefield image. Scale: 200 nm. Right - Cardiomyocyte stained against α-actinin, displaying its periodic structure localised at the ends of sarcomeres (Z-discs). (left) Widefield fluorescence of JF549 (right) dSTORM super-resolution image (right). Scale: 1 μm.

All images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou

DRAQ™ and CyTRAK Orange™ Dyes from Novus

Our sister brand Novus offers nuclear (DRAQ5™ and DRAQ7™), cytosolic (DRAQ9™) and whole cell (CyTRAK Orange™) dyes for flow cytometry, fluorescence microscopy, and high content screening. These dyes are photostable and suitable for use in live or fixed cells.


Bio-Techne Antibodies for Immunofluorescence Assays

Bio-Techne family brands R&D Systems™ and Novus Biologicals provide a comprehensive range of the highest quality antibodies to support your research. We offer portfolio of over 40,000 IHC validated antibodies covering common and niche targets.

Free Resources

Tocris is delighted to provide free scientific posters and reviews, all written by scientific experts in their fields. We also write and produce in-house product guides and bulletins, which give an overview of how our products are used within research including, cardiovascular, stem cells, neuroscience and cancer, among others. Please request a free copy or download a PDF today! Also check our latest blog posts following current scientific trends.

Literature for Imaging Applications

Tocris offers the following scientific literature for Imaging Applications to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals