Janelia Fluor® 525, SE

Pricing Availability Delivery Time Qty
Cat.No. 6296 - Janelia Fluor 525, SE | C31H21F4N3O7
Description: Cell permeable yellow fluorescent dye; supplied as NHS ester
Alternative Names: JF 252,NHS
Chemical Name: 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt
Datasheet
Citations
Reviews
Literature

Biological Activity

Yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Compatible with self-labeling tag systems, e.g.HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 525 nm; emission maximum = 549 nm; quantum yield = 0.91; Extinction coefficient = 122,000 M-1cm-1.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Janelia Fluor® 525, SE Dye Spectra

Spectra charts

Technical Data

M. Wt 623.51
Formula C31H21F4N3O7
Storage Store at -20°C
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 62.35 100

Preparing Stock Solutions

The following data is based on the product molecular weight 623.51. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.6 mL 8.02 mL 16.04 mL
5 mM 0.32 mL 1.6 mL 3.21 mL
10 mM 0.16 mL 0.8 mL 1.6 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 14 987 PMID: 28869757


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Literature in this Area

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New Product Guide (Spring/Summer 2018)

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