Janelia Fluor® 525, SE

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Description: Yellow dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM. Suitable for live cell imaging
Alternative Names: JF 525,NHS
Chemical Name: 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt
Protocols (1)
Literature (1)

Biological Activity for Janelia Fluor® 525, SE

Key information: Janelia Fluor® 525, SE is a yellow fluorescent dye; supplied with an NHS ester reactive for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques, including dSTORM (in both live and fixed cells). Janelia Fluor® 525, SE is cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; A280 correction factor = 0.185; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 525, SE

Janelia Fluor<sup>®</sup> 525, SE Dye Spectra
Emission Color Yellow
λabs 525 nm
λem 549 nm
Extinction Coefficient (ε) 122,000 M-1cm-1
Quantum Yield (φ) 0.91
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.185
Application Confocal Microscopy, Super Resolution Microscopy (SRM)
Alternative For Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Janelia Fluor® 525, SE

M. Wt 623.51
Formula C31H21F4N3O7
Storage Store at -20°C
CAS Number 2975167-21-4
PubChem ID 134160239
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 525, SE

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 31.18 50

Preparing Stock Solutions for Janelia Fluor® 525, SE

The following data is based on the product molecular weight 623.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.21 mL 16.04 mL 32.08 mL
2.5 mM 0.64 mL 3.21 mL 6.42 mL
5 mM 0.32 mL 1.6 mL 3.21 mL
25 mM 0.06 mL 0.32 mL 0.64 mL

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Product Datasheets for Janelia Fluor® 525, SE

Certificate of Analysis / Product Datasheet
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References for Janelia Fluor® 525, SE

References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 14 987 PMID: 28869757

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor® 525, SE, please let us know.

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Citations for Janelia Fluor® 525, SE

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Protocols for Janelia Fluor® 525, SE

The following protocol features additional information for the use of Janelia Fluor® 525, SE (Cat. No. 6296).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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