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Submit ReviewImmunoFect STAR™ Antibody Transfection Reagent New
Description: Reagent capable of efficiently delivering functional antibodies into the cytosol of cells
Biological Activity
ImmunoFect STAR™ Antibody Transfection Reagent is a highly efficient antibody transfection reagent designed for intracellular delivery of antibodies into live cells. ImmunoFect STAR™ Antibody Transfection Reagent enables functional studies of intracellular targets by facilitating direct antibody access to the cytosol. The delivery mechanism is optimized for high efficiency while maintaining cell viability.
Please see the protocol for further information on how to use ImmunoFect STAR™ Antibody Transfection Reagent.
This compound is supplied pre-dissolved in ethanol (10 mg/mL).
Scientific Data
Application of ImmunoFect STAR™ Antibody Transfection Reagent. Cytosolic delivery of anti-NUP153 antibody targeting the nuclear pore complex. HeLa cells were treated with anti-NUP153/ ImmunoFect STAR™ reagent complexes for 24 h, anti-NUP153 10 μg/mL, and ImmunoFect STAR™ reagent was 8 μg/mL). Cells were then washed, permeabilized and stained with AF647 conjugated anti-rabbit IgG and Hoechst before imaging and colocalization measurements.
Application of ImmunoFect STAR™ Antibody Transfection Reagent. Cytosolic delivery of native antibodies, fluorescence imaging was conducted after HeLa cells were treated with IgG-FITC/ ImmunoFect STAR™ reagent complexes for 4 h, IgG-FITC was 8 μg/mL, and ImmunoFect STAR™ reagent was 8 μg/mL (n = 3). Scale bar, 25 μm
Technical Data
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solubility | Soluble in ethanol (supplied pre-dissolved in anhydrous ethanol, 10mg/ml) |
Product Datasheets
References
References are publications that support the biological activity of the product.
Chen et al (2023) Exploring the effect of amphiphile architecture on intracellular protein delivery capacity: dimeric versus trimeric amphiphiles. ACS Appl.Mater.Interfaces 15 26328 PMID: 37219911
If you know of a relevant reference for ImmunoFect STAR™ Antibody Transfection Reagent, please let us know.
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Citations for ImmunoFect STAR™ Antibody Transfection Reagent
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