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Tyramide Signal Amplification (TSA), also known as Catalyzed Reported Deposition (CARD), offers an effective way to efficiently enhance signal and detection capabilities for low-abundance targets in immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) applications.
|Cat. No.||Product Name / Activity|
|Reagent widely used for signal amplification in IHC and FISH|
|6457||Cyanine 3 Tyramide|
|Orange fluorescent reagent widely used for signal amplification in IHC and FISH|
|6458||Cyanine 5 Tyramide|
|Red fluorescent reagent widely used for signal amplification in IHC and FISH|
|Green fluorescent reagent widely used for signal amplification in IHC and FISH|
Tyramide Signal Amplification (TSA), also known as catalyzed reported deposition (CARD), offers an effective way to efficiently boost your signal and detection capabilities for low-abundance targets in IHC, ICC and FISH. HRP catalyzes the conversion of labeled inactive tyramide into a reactive radical, which then binds to adjacent tyrosine residues greatly enhancing the density of labeling. Tyramide reagents can be conjugated with a dye for fluorescent detection, or a hapten for antibody-based probing.
TSA can be used to enhance the signal of multiple targets on one sample by deactivating HRP using a peroxidase quenching buffer, then carrying out another round of TSA using a different set of antibodies from a different host species. Alternatively, the antibodies for the initial round of TSA can be stripped, leaving the covalently bound tyramides. TSA can then be repeated for the desired number of targets.
Figure 1: Tyramide Signal Amplification Principles: A primary and secondary antibody are used to label a tissue or cell sample. The secondary antibody is pre-conjugated to horseradish peroxidase (HRP), which in the presence of H2O2, catalyzes a labeled tyramide substrate into a highly reactive species that covalently bind to tyrosine residues on the proteins in close proximity to the antibodies and HRP, thus providing signal amplification.
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