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Tyramide Signal Amplification (TSA), also known as Catalyzed Reported Deposition (CARD), offers an effective way to efficiently enhance signal and detection capabilities for low-abundance targets in immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) applications.
|Cat. No.||Product Name / Activity|
|Reagent widely used for signal amplification in IHC and FISH|
|6457||Cyanine 3 Tyramide|
|Orange fluorescent reagent widely used for signal amplification in IHC and FISH|
|6458||Cyanine 5 Tyramide|
|Red fluorescent reagent widely used for signal amplification in IHC and FISH|
|Green fluorescent reagent widely used for signal amplification in IHC and FISH|
Tyramide Signal Amplification (TSA), also known as catalyzed reported deposition (CARD), offers an effective way to efficiently boost your signal and detection capabilities for low-abundance targets in IHC, ICC and FISH. HRP catalyzes the conversion of labeled inactive tyramide into a reactive radical, which then binds to adjacent tyrosine residues greatly enhancing the density of labeling. Tyramide reagents can be conjugated with a dye for fluorescent detection, or a hapten for antibody-based probing.
TSA can be used to enhance the signal of multiple targets on one sample by deactivating HRP using a peroxidase quenching buffer, then carrying out another round of TSA using a different set of antibodies from a different host species. Alternatively, the antibodies for the initial round of TSA can be stripped, leaving the covalently bound tyramides. TSA can then be repeated for the desired number of targets.
Figure 1: Tyramide Signal Amplification Principles: A primary and secondary antibody are used to label a tissue or cell sample. The secondary antibody is pre-conjugated to horseradish peroxidase (HRP), which in the presence of H2O2, catalyzes a labeled tyramide substrate into a highly reactive species that covalently bind to tyrosine residues on the proteins in close proximity to the antibodies and HRP, thus providing signal amplification.
Bio-Techne family brands R&D Systems™ and Novus Biologicals provide the highest quality antibodies to support your research. We offer a comprehensive portfolio of over 40,000 IHC validated antibodies covering popular and unique targets. Our products have been cited over 7,000 times in peer-reviewed journals.
This handbook provides a comprehensive introduction to IHC that is well-suited for researchers new to the technique or experienced scientists looking for a quick refresher. Download or request a copy.
Tocris offers the following scientific literature for Tyramide Signaling Amplification (TSA) to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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