You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Janelia Fluor® 669, SE
Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Application of Janelia Fluor® 669, SE dye on Tubulin
Application of Janelia Fluor® 669, SE dye on Tubulin; Left: Microtubules were labeled with anti-α-tubulin primary antibodies. The secondary antibodies were conjugated to JF 669. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line forJF 669. Right: Enlarged portion of left-hand image.
Images kindly provided by Dr Kirstin Elgass, Monash University
|Extinction coefficient (ε)||116,000 M-1cm-1|
|Quantum yield (φ)||0.37|
|Closest Laser line||640 nm|
|Alternative for||Alexa Fluor® 660, Atto 655, Atto 665*, CF660R|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectra Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 693.77. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||7.21 mL||36.03 mL||72.07 mL|
|1 mM||1.44 mL||7.21 mL||14.41 mL|
|2 mM||0.72 mL||3.6 mL||7.21 mL|
|10 mM||0.14 mL||0.72 mL||1.44 mL|
References are publications that support the biological activity of the product.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
If you know of a relevant reference for Janelia Fluor® 669, SE, please let us know.
Keywords: Janelia Fluor 669, SE, Janelia Fluor 669, SE supplier, JF669, NHS, Janelia, Fluor, 669-SE, fluorescent, dyes, ester, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, JF, 669,, Dyes, 6420, Tocris Bioscience
Citations for Janelia Fluor® 669, SE
Citations are publications that use Tocris products.
Currently there are no citations for Janelia Fluor® 669, SE. Do you know of a great paper that uses Janelia Fluor® 669, SE from Tocris? Please let us know.
Reviews for Janelia Fluor® 669, SE
There are currently no reviews for this product. Be the first to review Janelia Fluor® 669, SE and earn rewards!
Have you used Janelia Fluor® 669, SE?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Protocols for Janelia Fluor® 669, SE
The following protocol features additional information for the use of Janelia Fluor® 669, SE (Cat. No. 6420).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Fall/Winter 2018/2019)New
Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells