Janelia Fluor® 669, SE

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Cat.No. 6420 - Janelia Fluor 669, SE | C34H30F3N3O6SSi | CAS No. 2127150-20-1
Description: Cell permeable far red fluorescent dye; supplied as NHS ester
Alternative Names: JF 669, NHS
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Datasheet
Citations
Reviews
Protocols (1)
Literature

Biological Activity

Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor® 669, SE dye on Tubulin

Janelia Fluor® 669, SE Dye-labeled Tubulin Janelia Fluor® 669, SE Dye-labeled Tubulin

Application of Janelia Fluor® 669, SE dye on Tubulin; Left: Microtubules were labeled with anti-α-tubulin primary antibodies. The secondary antibodies were conjugated to JF 669. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line forJF 669. Right: Enlarged portion of left-hand image.

Images kindly provided by Dr Kirstin Elgass, Monash University


Janelia Fluor® 669, SE Dye Spectra

Spectra charts

Technical Data

M. Wt 693.77
Formula C34H30F3N3O6SSi
Storage Store at -20°C
CAS Number 2127150-20-1
Smiles C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 34.69 50

Preparing Stock Solutions

The following data is based on the product molecular weight 693.77. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.88 mL 14.41 mL 28.83 mL
2.5 mM 0.58 mL 2.88 mL 5.77 mL
5 mM 0.29 mL 1.44 mL 2.88 mL
25 mM 0.06 mL 0.29 mL 0.58 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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References

References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757


If you know of a relevant reference for Janelia Fluor® 669, SE, please let us know.

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Citations for Janelia Fluor® 669, SE

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Protocols for Janelia Fluor® 669, SE

The following protocol features additional information for the use of Janelia Fluor® 669, SE (Cat. No. 6420).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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