Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewJanelia Fluor® 669, NHS ester
Description: Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Alternative Names: JF 669,NHS
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 669, NHS ester
Key Information: Janelia Fluor® 669, NHS ester is a red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 669 nm and 682 nm, respectively; quantum yield = 0.37; extinction coefficient = 116,000 M-1cm-1; A280 correction factor = 0.043. Retains 97% fluorescence after 30 bleaching cycles.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 669, SE.
Scientific Data
Confocal Microscopy
View Larger
View Larger
Applications on Tubulin for Janelia Fluor® 669, NHS ester. Application of Janelia Fluor® 669, NHS ester dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, NHS ester. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 669, NHS ester
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 669 nm |
| λem | 682 nm |
| Extinction Coefficient (ε) | 116,000 M-1cm-1 |
| Quantum Yield (φ) | 0.37 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.043 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
| Alternative For | Alexa Fluor® 660, Atto 655, Atto 665, CF660R |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Janelia Fluor® 669, NHS ester
| M. Wt | 693.77 |
| Formula | C34H30F3N3O6SSi |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2127150-20-1 |
| PubChem ID | 132470916 |
| InChI Key | QFHKALIQFJQJKE-UHFFFAOYSA-N |
| Smiles | C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 669, NHS ester
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.88 | 20 |
Preparing Stock Solutions for Janelia Fluor® 669, NHS ester
The following data is based on the product molecular weight 693.77. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.21 mL | 36.03 mL | 72.07 mL |
| 1 mM | 1.44 mL | 7.21 mL | 14.41 mL |
| 2 mM | 0.72 mL | 3.6 mL | 7.21 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.44 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Janelia Fluor® 669, NHS ester
References for Janelia Fluor® 669, NHS ester
References are publications that support the biological activity of the product.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
Grimm et al (2017) General synthetic method for Si-Fluoresceins and Si-Rhodamines. ACS Cent.Sci. 3 975 PMID: 28979939
Grimm et al (2020) A general method to optimize and functionalize red-shifted rhodamine dyes. Nat.Methods 17 815 PMID: 32719532
If you know of a relevant reference for Janelia Fluor® 669, NHS ester, please let us know.
View Related Products by Target
Keywords: Janelia Fluor 669, NHS ester, Janelia Fluor 669, NHS ester supplier, JF669-NHS, ester, Janelia, Fluor, 669-NHS, fluorescent, dyes, NHS, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, 669,, SE, JF, Amine, Reactive, Dyes, 6420, Tocris Bioscience
1 Citation for Janelia Fluor® 669, NHS ester
Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 669, NHS ester include:
Hui et al (2021) EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization. Cell 184 6361-6377.e24 PMID: 34875226
Do you know of a great paper that uses Janelia Fluor® 669, NHS ester from Tocris? Please let us know.
Reviews for Janelia Fluor® 669, NHS ester
There are currently no reviews for this product. Be the first to review Janelia Fluor® 669, NHS ester and earn rewards!
Have you used Janelia Fluor® 669, NHS ester?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Protocols for Janelia Fluor® 669, NHS ester
The following protocol features additional information for the use of Janelia Fluor® 669, NHS ester (Cat. No. 6420).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents