Janelia Fluor® 549, SE

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Cat.No. 6147 - Janelia Fluor 549, SE | C31H25N3O7 | CAS No. 1811539-32-8
Description: Cell permeable fluorescent dye; supplied as NHS ester
Alternative Names: JF 549, NHS
Chemical Name: 3,6-Di-1-azetidinyl-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt
Datasheet
Citations
Literature

Biological Activity

Fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Compatible with self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169. Janelia Fluor® 646 SE (Cat.No. 6148) also available.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor® Dyes in Cardiac Tissue

Application of Janelia Fluor® Dyes in Cardiac Tissue

Application of Janelia Fluor® Dyes in Cardiac Tissue - Left image - Widefield fluorescence image of muscle tissue from an adult pig heart, displaying the distribution of collagen VI in the interstitial space between heart muscle cells. This sample was labelled with a primary antibody against collagen VI and a secondary antibody conjugated to JF549 (Cat.No. 6147). Scale bar: 50 μm. Middle image - An adult pig heart tissue section, 10 μm thick, labelled with antibodies against SERCA2ATPase, exhibits the intricate structure of the sarcoplasmic reticulum. A super-resolution image (top panel), obtained by exploiting spontaneous photo-switching of JF549 (dSTORM), shows far superior detail than the diffraction limited widefield image (bottom). Scale bar: 200 nm. Right image - A single isolated Wistar rat cardiomyocyte stained against α-actinin, displaying its periodic structure localised at the ends of sarcomeres (Z-discs). Widefield fluorescence of JF549 (left panel), prior to illuminating with a 561 nm laser to induce photoswitching and produce a dSTORM super-resolution image (right). Scale bar: 1 μm.

All images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Technical Data

M. Wt 551.56
Formula C31H25N3O7
Storage Store at -20°C
CAS Number 1811539-32-8
PubChem ID 124201856
InChI Key DWUIXAGWHCMXHN-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 55.16 100

Preparing Stock Solutions

The following data is based on the product molecular weight 551.56. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.81 mL 9.07 mL 18.13 mL
5 mM 0.36 mL 1.81 mL 3.63 mL
10 mM 0.18 mL 0.91 mL 1.81 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID: 26324940

Knight et al (2015) Dynamics of CRISPR-Cas9 genome interrogation in living cells. Science 350 823 PMID: 26564855

Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745


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