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Developed by Professor Luke Lavis and his team at the Janelia Research Campus, Janelia Fluor® (JF) dyes provide scientists with an exceptional palette of bright, photostable and cell permeable fluorophores for a broad range of applications, including super-resolution microscopy. The Janelia Fluor® range includes products that possess different specific and useful properties, such as: fluorogenicity; spontaneous blinking (for facile single-molecule localization microscopy (SMLM)); and photoactivation.
The full range of Janelia Fluor® dyes are available from Tocris with a selection of reactive groups for conjugation to biomolecules. Conjugation protocols available. Let us know if you require a different modification, Custom Chemistry services are available.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister brand Novus Biologicals.
Engineered for enhanced brightness and longer fluorescence lifetime, Janelia Fluor® (JF) dyes offer significant advantages over spectrally similar dyes and incorporate additional properties for application in specific super-resolution microscopy techniques.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Figure 1: Application of Janelia Fluor® Dyes in Cardiac Tissue. Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. Left: JF 646 (Cat. No. 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm. Right: - Displays the distribution of collagen VI in the interstitial space between muscle cells. Labeled with a primary ab against collagen VI and a secondary ab conjugated to JF 549 (Cat. No. 6147). Scale: 50 μm.
Images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.
Tocris has an exclusive range of Janelia Fluor® dye-based probes, for labeling cellular targets. These include Taxol Janelia Fluor® 646 (Cat. No. 6266), a fluorogenic probe for microtubule staining, which is non-fluorescent until bound to microtubules, enabling no-wash experiments (protocol available). BOP-JF646 (Cat. No. 6997), a fluorogenic red fluorescent dual α9β1/α4β1 integrins inhibitor, which is exceptionally photostable and BOP-JF549 (Cat. No. 6996), a yellow fluorogenic fluorescent dual α9β1/α4β1 integrin inhibitor. All three of these probes are fluorogenic, meaning they fluoresce only when bound to their target, allowing for hassle-free no-wash experiments, with bright, low noise signals, perfect for sensitive or low expression experiments.
Figure 2: Left: Application of Janelia Fluor® Probes Left: COS7 cells were labeled with 3 μM Taxol Janelia Fluor® 646 (Cat. No. 6266) for one hour at 37°C. Images were taken on a Leica TCS SP8 Confocal Laser Scanning Microscope. Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Evelina Lucinskaite, Anna Meletiou and Alexander Clowsley. Middle: Immortalized human keratinocyte cells expressing a GFP-tagged α4 integrin were treated with 10 μM BOP-Janelia Fluor® 646 (Cat. No. 6997). Cells were incubated at 37°C and imaged using a Nikon A1R confocal microscope. Scale bar = 10 μm. Right: Immortalized human keratinocytes expressing a GFP-tagged α4 integrin were incubated with 10 μm BOP-Janelia Fluor® 549 (Cat. No. 6996). Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm. Middle and right images kindly provided by Willow Hight-Warburton, Kings College London.
Tocris offers the following scientific literature for Janelia Fluor® Dyes to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes