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Polyamine Supplement x1000 (lyophilized)
Polyamine Supplement x1000 (lyophilized) is a media supplement comprising a lyophilized mixture of Putrescine, Spermine (Cat. No. 0958) and Spermidine. The product is used as a component of the CEPT cocktail, in combination with Emricasan (Cat. No. 7310), Chroman 1 (Cat. No. 7163) and trans-ISRIB (Cat. No. 5284) to enhance viability of human pluripotent stem cells (hPSCs) and their differentiated progeny. Polyamine Supplement x1000 (lyophilized) also improves survival of pluripotent and differentiated cells during cryopreservation, organoid formation, single-cell cloning, and genome editing.
NOTE: This reagent is provided lyophilized. To use prepare a 1000x stock solution by reconstituting vial contents in 5 mL of ddH2O and filter sterilize before use. Add 1 mL of sterile resuspended polyamines supplement (1000x stock) per 1 L of media.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Chen et al (2021) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.