Chroman 1

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Cat.No. 7163 - Chroman 1 | C24H28N4O4.2HCl
Description: Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogeneisis
Chemical Name: (3S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2H-1-benzopyran-3-carboxamide dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (6)

Biological Activity

Highly potent and selective ROCK2 inhibitor (IC50 values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Exhibits selectivity over a range of related kinases including PKA, AKT1 and MRCK (IC50 values are >20000, >20000 and 150 nM respectively). Improves cell survival after cryogenesis. Also promotes survival of stem cells in culture.

Technical Data

M. Wt 509.43
Formula C24H28N4O4.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
InChI Key ROFMCPHQNWGXGE-SFHVURJKSA-N
Smiles COC1=CC=C2OC[C@@H](C(NC3=C(C=C(C4=CNN=C4)C=C3)OCCN(C)C)=O)CC2=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 50.94 100
water 2.55 5

Preparing Stock Solutions

The following data is based on the product molecular weight 509.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.96 mL 9.81 mL 19.63 mL
5 mM 0.39 mL 1.96 mL 3.93 mL
10 mM 0.2 mL 0.98 mL 1.96 mL
50 mM 0.04 mL 0.2 mL 0.39 mL

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References

References are publications that support the biological activity of the product.

Chen et al (2011) Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun. 2 73

Chen et al (2019) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. BioRxiv - Paper not yet peer reviewed.


If you know of a relevant reference for Chroman 1, please let us know.

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Citations for Chroman 1

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


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Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

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Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells

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Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.