Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewChroman 1
Description: Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogeneisis
Chemical Name: (3S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2H-1-benzopyran-3-carboxamide dihydrochloride
Purity: ≥98% (HPLC)
Biological Activity
Highly potent and selective ROCK2 inhibitor (IC50 values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Exhibits selectivity over a range of related kinases including PKA, AKT1 and MRCK (IC50 values are >20000, >20000 and 150 nM respectively). Improves cell survival after cryogenesis. Also promotes survival of stem cells in culture.
Compound Libraries
Chroman 1 is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 509.43 |
| Formula | C24H28N4O4.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| InChI Key | ROFMCPHQNWGXGE-SFHVURJKSA-N |
| Smiles | COC1=CC=C2OC[C@@H](C(NC3=C(C=C(C4=CNN=C4)C=C3)OCCN(C)C)=O)CC2=C1.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 50.94 | 100 | |
| water | 2.55 | 5 |
Preparing Stock Solutions
The following data is based on the product molecular weight 509.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.96 mL | 9.81 mL | 19.63 mL |
| 5 mM | 0.39 mL | 1.96 mL | 3.93 mL |
| 10 mM | 0.2 mL | 0.98 mL | 1.96 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.39 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets
References
References are publications that support the biological activity of the product.
Chen et al (2011) Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun. 2 73
Chen et al (2019) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. BioRxiv - Paper not yet peer reviewed.
If you know of a relevant reference for Chroman 1, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Chroman 1, Chroman 1 supplier, Selective, p160, ROCK, inhibitors, inhibits, Rho-Kinase, Rho-Associated, Coiled-Coil, Kinases, ROK, stem, cells, organoids, induced, pluripotent, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell, freezing, media, cryogenesis, self, renewed, Rho-kinases, Stem, Cell, Proliferation, Reprogramming, Organoids, Neural, Cells, 7163, Tocris Bioscience
Citations for Chroman 1
Citations are publications that use Tocris products.
Currently there are no citations for Chroman 1. Do you know of a great paper that uses Chroman 1 from Tocris? Please let us know.
Reviews for Chroman 1
There are currently no reviews for this product. Be the first to review Chroman 1 and earn rewards!
Have you used Chroman 1?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cancer Research Product Guide
A collection of over 750 products for cancer research, the guide includes research tools for the study of:
- Cancer Metabolism
- Epigenetics in Cancer
- Receptor Signaling
- Cell Cycle and DNA Damage Repair
- Angiogenesis
- Invasion and Metastasis
Cardiovascular Research Product Guide
A collection of over 250 products for cardiovascular research, the guide includes research tools for the study of:
- Hypertension
- Thrombosis and Hemostasis
- Atherosclerosis
- Myocardial Infarction
- Ischemia/Reperfusion Injury
- Arrhythmias
- Heart Failure
Kinases Product Listing
A collection of over 400 products for kinase research, the listing includes inhibitors of:
- Receptor Tyrosine Kinases
- Protein Kinases A, C, D and G
- PI-3 Kinase, Akt and mTOR
- MAPK Signaling
- Receptor Serine/Threonine Kinases
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.