Methylene Blue

Pricing Availability Delivery Time Qty
Cat.No. 3213 - Methylene Blue | C16H18ClN3S | CAS No. 61-73-4
Description: Biological stain
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
Datasheet
Citations
Literature

Biological Activity

Biological stain and redox indicator. Inhibits tau filament formation (IC50 = 1.9 μM). Also inhibits soluble guanylyl cyclase.

Technical Data

M. Wt 319.85
Formula C16H18ClN3S
Storage Store at RT
CAS Number 61-73-4
PubChem ID 6099
InChI Key CXKWCBBOMKCUKX-UHFFFAOYSA-M
Smiles CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.2 10
ethanol 3.2 10
water 3.2 10

Preparing Stock Solutions

The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.13 mL 15.63 mL 31.26 mL
5 mM 0.63 mL 3.13 mL 6.25 mL
10 mM 0.31 mL 1.56 mL 3.13 mL
50 mM 0.06 mL 0.31 mL 0.63 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene boue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642

Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092

Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Neurobiology 93 11213 PMID:


If you know of a relevant reference for Methylene Blue, please let us know.

Keywords: Methylene Blue, supplier, Biological, stain, Soluble, guanylyl, cyclases, inhibitors, inhibits, tau, filaments, aggregation, Reagents, sGC, Tubulin, Microtubules, Nitric, Oxide, Signaling, Signalling, Labels, and, Stains, Tocris Bioscience

Citations for Methylene Blue

Citations are publications that use Tocris products.

Currently there are no citations for Methylene Blue. Do you know of a great paper that uses Methylene Blue from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for Methylene Blue

Protocols

TODO: Add Protocols