Methylene Blue

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Cat.No. 3213 - Methylene Blue | C16H18ClN3S | CAS No. 61-73-4
Description: Biological stain
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride

Biological Activity

Biological stain and redox indicator. Inhibits tau filament formation (IC50 = 1.9 μM). Also inhibits soluble guanylyl cyclase.

Technical Data

M. Wt 319.85
Formula C16H18ClN3S
Storage Store at RT
CAS Number 61-73-4
PubChem ID 6099
Smiles CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.2 10
ethanol 3.2 10
water 3.2 10

Preparing Stock Solutions

The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.13 mL 15.63 mL 31.26 mL
5 mM 0.63 mL 3.13 mL 6.25 mL
10 mM 0.31 mL 1.56 mL 3.13 mL
50 mM 0.06 mL 0.31 mL 0.63 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene boue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642

Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092

Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Neurobiology 93 11213 PMID:

If you know of a relevant reference for Methylene Blue, please let us know.

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