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Submit ReviewMethylene Blue
Description: Biological stain; also neuroprotective and antimalarial
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
Biological Activity for Methylene Blue
Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC50 values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC50 = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.
Technical Data for Methylene Blue
| M. Wt | 319.85 |
| Formula | C16H18ClN3S |
| Storage | Store at RT |
| CAS Number | 61-73-4 |
| PubChem ID | 6099 |
| InChI Key | CXKWCBBOMKCUKX-UHFFFAOYSA-M |
| Smiles | CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Methylene Blue
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.2 | 10 | |
| DMSO | 3.2 | 10 |
Preparing Stock Solutions for Methylene Blue
The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.26 mL | 156.32 mL | 312.65 mL |
| 0.5 mM | 6.25 mL | 31.26 mL | 62.53 mL |
| 1 mM | 3.13 mL | 15.63 mL | 31.26 mL |
| 5 mM | 0.63 mL | 3.13 mL | 6.25 mL |
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Product Datasheets for Methylene Blue
References for Methylene Blue
References are publications that support the biological activity of the product.
Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642
Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092
Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc.Natl.Acad.Sci.U.S.A. 93 11213 PMID: 8855335
Atamna et al (2008) Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 22 703 PMID: 17928358
Mayer et al (1993) Inhibition of nitric oxide synthesis by methylene blue. Biochem.Pharmacol. 45 367 PMID: 7679577
Poteet et al (2012) Neuroprotective actions of methylene blue and its derivatives. PLoS One 7 PMID: 23118969
Wen et al (2011) Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. J.Biol.Chem. 286 16504 PMID: 21454572
If you know of a relevant reference for Methylene Blue, please let us know.
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Citations for Methylene Blue
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Reviews for Methylene Blue
Average Rating: 5 (Based on 1 Review.)
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Methylene blue was used to prepare Eosin Methylene Blue agar plate.
By
Anonymous on 11/13/2018
Species: Human
Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.
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