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Submit ReviewMethylene Blue
Description: Biological stain
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
Biological Activity
Biological stain and redox indicator. Inhibits tau filament formation (IC50 = 1.9 μM). Also inhibits soluble guanylyl cyclase.
Technical Data
| M. Wt | 319.85 |
| Formula | C16H18ClN3S |
| Storage | Store at RT |
| CAS Number | 61-73-4 |
| PubChem ID | 6099 |
| InChI Key | CXKWCBBOMKCUKX-UHFFFAOYSA-M |
| Smiles | CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.2 | 10 | |
| DMSO | 3.2 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.26 mL | 156.32 mL | 312.65 mL |
| 0.5 mM | 6.25 mL | 31.26 mL | 62.53 mL |
| 1 mM | 3.13 mL | 15.63 mL | 31.26 mL |
| 5 mM | 0.63 mL | 3.13 mL | 6.25 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene boue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642
Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092
Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Neurobiology 93 11213
If you know of a relevant reference for Methylene Blue, please let us know.
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Keywords: Methylene Blue, Methylene Blue supplier, Biological, stain, Soluble, guanylyl, cyclases, inhibitors, inhibits, tau, filaments, aggregation, Reagents, sGC, Tubulin, Microtubules, Nitric, Oxide, Signaling, Signalling, Guanylyl, Cyclase, Other, Mitosis, Labels, and, Stains, 3213, Tocris Bioscience
Citations for Methylene Blue
Citations are publications that use Tocris products.
Currently there are no citations for Methylene Blue. Do you know of a great paper that uses Methylene Blue from Tocris? Please let us know.
Reviews for Methylene Blue
Average Rating: 5 (Based on 1 Review.)
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Methylene blue was used to prepare Eosin Methylene Blue agar plate: Excellent publishable, performed ideally.
By
Anonymous on 11/13/2018
Species: Human
Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.
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