Methylene Blue

Pricing Availability   Qty
Cat.No. 3213 - Methylene Blue | C16H18ClN3S | CAS No. 61-73-4
Description: Biological stain
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
Reviews (1)
Literature (1)

Biological Activity for Methylene Blue

Methylene Blue is a biological stain and redox indicator. Inhibits tau filament formation (IC50 = 1.9 μM). Also inhibits soluble guanylyl cyclase.

Technical Data for Methylene Blue

M. Wt 319.85
Formula C16H18ClN3S
Storage Store at RT
CAS Number 61-73-4
PubChem ID 6099
Smiles CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Methylene Blue

Solvent Max Conc. mg/mL Max Conc. mM
water 3.2 10
DMSO 3.2 10

Preparing Stock Solutions for Methylene Blue

The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.26 mL 156.32 mL 312.65 mL
0.5 mM 6.25 mL 31.26 mL 62.53 mL
1 mM 3.13 mL 15.63 mL 31.26 mL
5 mM 0.63 mL 3.13 mL 6.25 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Methylene Blue

References for Methylene Blue

References are publications that support the biological activity of the product.

Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene boue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642

Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092

Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Neurobiology 93 11213

If you know of a relevant reference for Methylene Blue, please let us know.

Keywords: Methylene Blue, Methylene Blue supplier, Biological, stain, Soluble, guanylyl, cyclases, inhibitors, inhibits, tau, filaments, aggregation, Reagents, sGC, Tubulin, Microtubules, Nitric, Oxide, Signaling, Signalling, Guanylyl, Cyclase, Other, Mitosis, Labels, and, Stains, Histology, 3213, Tocris Bioscience

Citations for Methylene Blue

Citations are publications that use Tocris products.

Currently there are no citations for Methylene Blue. Do you know of a great paper that uses Methylene Blue from Tocris? Please let us know.

Reviews for Methylene Blue

Average Rating: 5 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Methylene Blue?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Methylene blue was used to prepare Eosin Methylene Blue agar plate.
By Anonymous on 11/13/2018
Species: Human

Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals