Methylene Blue

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Cat.No. 3213 - Methylene Blue | C16H18ClN3S | CAS No. 61-73-4
Description: Biological stain
Chemical Name: 3,7-bis(Dimethylamino)phenazathionium chloride
Datasheet
Citations
Reviews (1)
Literature

Biological Activity

Biological stain and redox indicator. Inhibits tau filament formation (IC50 = 1.9 μM). Also inhibits soluble guanylyl cyclase.

Technical Data

M. Wt 319.85
Formula C16H18ClN3S
Storage Store at RT
CAS Number 61-73-4
PubChem ID 6099
InChI Key CXKWCBBOMKCUKX-UHFFFAOYSA-M
Smiles CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 3.2 10
DMSO 3.2 10

Preparing Stock Solutions

The following data is based on the product molecular weight 319.85. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.26 mL 156.32 mL 312.65 mL
0.5 mM 6.25 mL 31.26 mL 62.53 mL
1 mM 3.13 mL 15.63 mL 31.26 mL
5 mM 0.63 mL 3.13 mL 6.25 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Hwang et al (1998) Comparison of two soluble guanylyl cyclase inhibitors, methylene boue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 125 1158 PMID: 9863642

Taniguchi et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 280 7614 PMID: 15611092

Wischik et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Neurobiology 93 11213


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Keywords: Methylene Blue, Methylene Blue supplier, Biological, stain, Soluble, guanylyl, cyclases, inhibitors, inhibits, tau, filaments, aggregation, Reagents, sGC, Tubulin, Microtubules, Nitric, Oxide, Signaling, Signalling, Guanylyl, Cyclase, Other, Mitosis, Labels, and, Stains, 3213, Tocris Bioscience

Citations for Methylene Blue

Citations are publications that use Tocris products.

Currently there are no citations for Methylene Blue. Do you know of a great paper that uses Methylene Blue from Tocris? Please let us know.

Reviews for Methylene Blue

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Methylene blue was used to prepare Eosin Methylene Blue agar plate.
By Anonymous on 11/13/2018
Species: Human

Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.

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