Mitochondrial electron transport chain inhibitor (IC50 = 1.7 - 2.2 μM at complex I). Inhibits NADH oxidation by cardiac sarcoplasmic reticulum (IC50 = 3.4 nM). Commonly used pesticide and induces Parkinsonism in animal models. May also inhibit autophagy induction; blocks lysosomal degradation of autophagic vacuoles. Cell-permeable and brain penetrant.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 394.42. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.54 mL||12.68 mL||25.35 mL|
|5 mM||0.51 mL||2.54 mL||5.07 mL|
|10 mM||0.25 mL||1.27 mL||2.54 mL|
|50 mM||0.05 mL||0.25 mL||0.51 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Cherednichenko et al (2004) NADH oxidase activity of rat cardiac sarcoplasmic reticulum regulates calcium-induced calcium release. Circ.Res. 94 478 PMID: 14699012
Uversky (2004) Neurotoxicant-induced animal models of Parkinson's disease: understanding the role of rotenone, maneb and paraquat in neurodegeneration. Cell Tissue Res. 318 225 PMID: 15258850
Gomez et al (2007) Pesticides and impairment of mitochondrial function in relation with the parkinsonian syndrome. Front.Biosci. 12 1079 PMID: 17127363
Mader et al (2012) Rotenone inhibits autophagic flux prior to inducing cell death. ACS Chem.Neurosci. 3 1063 PMID: 23259041
If you know of a relevant reference for Rotenone, please let us know.
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Keywords: inhibitors inhibits complex I mitochondrial electron transport chain Oxidative phosphorylation parkinson's parkinsons parkinsonism complex I Oxidative Phosphorylation
1 Citation for Rotenone
Citations are publications that use Tocris products. Selected citations for Rotenone include:
Juliana et al (2012) Non-transcriptional priming and deubiquitination regulate NLRP3 inflammasome activation. Leukemia 287 36617 PMID: 22948162
Do you know of a great paper that uses Rotenone from Tocris? If so please let us know.
Literature in this Area
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